Gamma irradiation triggers a global stress response in Escherichia coli O157:H7 including base and nucleotides excision repair pathways

2020 ◽  
Vol 149 ◽  
pp. 104342
Author(s):  
Ghizlane Gaougaou ◽  
Shiv Shankar ◽  
Quentin Liot ◽  
Philippe Constant ◽  
Eric Déziel ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Stress Response ◽  
Gamma Irradiation ◽  
Excision Repair ◽  
Escherichia Coli O157 ◽  
Repair Pathways ◽  
Global Stress

Inactivation of Escherichia coli O157:H7 and Salmonella Typhimurium in black pepper and red pepper by gamma irradiation

2014 ◽  
Vol 172 ◽  
pp. 125-129 ◽  
Author(s):  
Won-Jae Song ◽  
Hye-Jung Sung ◽  
Sung-Youn Kim ◽  
Kwang-Pyo Kim ◽  
Sangryeol Ryu ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Salmonella Typhimurium ◽  
Gamma Irradiation ◽  
Black Pepper ◽  
Red Pepper ◽  
Escherichia Coli O157

Thermal and Starvation Stress Response of Escherichia coli O157:H7 Isolates Selected from Agricultural Environments

2016 ◽  
Vol 79 (10) ◽  
pp. 1673-1679 ◽  
Author(s):  
ACHYUT ADHIKARI ◽  
ANDY BARY ◽  
CRAIG COGGER ◽  
CALEB JAMES ◽  
GÜLHAN ÜNLÜ ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Heat Stress ◽  
Stress Response ◽  
Weibull Model ◽  
Stress Conditions ◽  
Inactivation Kinetics ◽  
Escherichia Coli O157 ◽  
Starvation Stress ◽  
E Coli ◽  
Response To Stress

ABSTRACT Pathogens exposed to agricultural production environments are subject to multiple stresses that may alter their survival under subsequent stress conditions. The objective of this study was to examine heat and starvation stress response of Escherichia coli O157:H7 strains isolated from agricultural matrices. Seven E. coli O157:H7 isolates from different agricultural matrices—soil, compost, irrigation water, and sheep manure—were selected, and two ATCC strains were used as controls. The E. coli O157:H7 isolates were exposed to heat stress (56°C in 0.1% peptone water for up to 1 h) and starvation (in phosphate-buffered saline at 37°C for 15 days), and their survival was examined. GInaFiT freeware tool was used to perform regression analyses of the surviving populations. The Weibull model was identified as the most appropriate model for response of the isolates to heat stress, whereas the biphasic survival curves during starvation were fitted using the double Weibull model, indicating the adaptation to starvation or a resistant subpopulation. The inactivation time during heating to achieve the first decimal reduction time (δ) calculated with the Weibull parameters was the highest (45 min) for a compost isolate (Comp60A) and the lowest (28 min) for ATCC strain 43895. Two of the nine isolates (ATCC 43895 and a manure isolate) had β < 1, indicating that surviving populations adapted to heat stress, and six strains demonstrated downward concavity (β > 1), indicating decreasing heat resistance over time. The ATCC strains displayed the longest δ2 (>1,250 h) in response to starvation stress, compared with from 328 to 812 h for the environmental strains. The considerable variation in inactivation kinetics of E. coli O157:H7 highlights the importance of evaluating response to stress conditions among individual strains of a specific pathogen. Environmental isolates did not exhibit more robust response to stress conditions in this study compared with ATCC strains.

scholarly journals Inactivation of Escherichia coli O157:H7 in hamburgers by gamma irradiation

2002 ◽  
Vol 33 (1) ◽  
pp. 53-56 ◽  
Author(s):  
Rodolfo R.O. Chirinos ◽  
Dirceu M. Vizeu ◽  
Maria Teresa Destro ◽  
Bernadette D.G.M. Franco ◽  
Mariza Landgraf
Keyword(s):  
Escherichia Coli ◽  
Gamma Irradiation ◽  
Escherichia Coli O157
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