Characterization and whole-genome sequencing of broad-host-range Salmonella-specific bacteriophages for bio-control

2020 ◽  
Vol 143 ◽  
pp. 104119
Author(s):  
Ping Li ◽  
Xiuzhong Zhang ◽  
Xianjun Xie ◽  
Zunfang Tu ◽  
Ju Gu ◽  
...  
Keyword(s):  
Whole Genome Sequencing ◽  
Host Range ◽  
Genome Sequencing ◽  
Broad Host Range ◽  
Whole Genome

scholarly journals Whole-Genome Sequencing of 10 Pseudomonas syringae Strains Representing Different Host Range Spectra: TABLE 1

2015 ◽  
Vol 3 (2) ◽  
Author(s):  
Claudia Bartoli ◽  
Sébastien Carrere ◽  
Jay Ram Lamichhane ◽  
Leonardo Varvaro ◽  
Cindy E. Morris
Keyword(s):  
Whole Genome Sequencing ◽  
Host Range ◽  
Pseudomonas Syringae ◽  
Genome Sequencing ◽  
Whole Genome

scholarly journals Evaluation of Molecular Methods for Identification of Salmonella Serovars

2016 ◽  
Vol 54 (8) ◽  
pp. 1992-1998 ◽  
Author(s):  
Catherine Yoshida ◽  
Simone Gurnik ◽  
Aaminah Ahmad ◽  
Travis Blimkie ◽  
Stephanie A. Murphy ◽  
...  
Keyword(s):  
Whole Genome Sequencing ◽  
Genome Sequencing ◽  
High Throughput ◽  
Molecular Methods ◽  
Outbreak Detection ◽  
Broad Host Range ◽  
Whole Genome ◽  
Content Type ◽  
Molecular Serotyping ◽  
Commercial Kits

Classification by serotyping is the essential first step in the characterization ofSalmonellaisolates and is important for surveillance, source tracking, and outbreak detection. To improve detection and reduce the burden of salmonellosis, several rapid and high-throughput molecularSalmonellaserotyping methods have been developed.The aim of this study was to compare three commercial kits, Salm SeroGen (Salm Sero-Genotyping AS-1 kit), Check&Trace (Check-Points), and xMAP (xMAPSalmonellaserotyping assay), to theSalmonellagenoserotyping array (SGSA) developed by our laboratory. They were assessed using a panel of 321 isolates that represent commonly reported serovars from human and nonhuman sources globally. The four methods correctly identified 73.8% to 94.7% of the isolates tested. The methods correctly identified 85% and 98% of the clinically importantSalmonellaserovars Enteritidis and Typhimurium, respectively. The methods correctly identified 75% to 100% of the nontyphoidal, broad host rangeSalmonellaserovars, including Heidelberg, Hadar, Infantis, Kentucky, Montevideo, Newport, and Virchow. The sensitivity and specificity ofSalmonellaserovars Typhimurium and Enteritidis ranged from 85% to 100% and 99% to 100%, respectively.It is anticipated that whole-genome sequencing will replace serotyping in public health laboratories in the future. However, at present, it is approximately three times more expensive than molecular methods. Until consistent standards and methodologies are deployed for whole-genome sequencing, data analysis and interlaboratory comparability remain a challenge. The use of molecular serotyping will provide a valuable high-throughput alternative to traditional serotyping. This comprehensive analysis provides a detailed comparison of commercial kits available for the molecular serotyping ofSalmonella.

scholarly journals Correction for Bartoli et al., Whole-Genome Sequencing of 10 Pseudomonas syringae Strains Representing Different Host Range Spectra: TABLE 1

2015 ◽  
Vol 3 (3) ◽  
Author(s):  
Claudia Bartoli ◽  
Sébastien Carrere ◽  
Jay Ram Lamichhane ◽  
Leonardo Varvaro ◽  
Cindy E. Morris
Keyword(s):  
Whole Genome Sequencing ◽  
Host Range ◽  
Pseudomonas Syringae ◽  
Genome Sequencing ◽  
Whole Genome

Utility of Whole Genome Sequencing in diagnosing complex disorders: lesson from renal tubular disorders

2018 ◽  
Author(s):  
Mark Stevenson ◽  
Alistair T Pagnamenta ◽  
Heather G Mack ◽  
Judith A Savige ◽  
Kate E Lines ◽  
...  
Keyword(s):  
Whole Genome Sequencing ◽  
Genome Sequencing ◽  
Whole Genome ◽  
Complex Disorders ◽  
Tubular Disorders ◽  
Renal Tubular Disorders ◽  
Renal Tubular

0306 Exploring the feasibility of using copy number variants as genetic markers through large-scale whole genome sequencing experiments

2016 ◽  
Vol 94 (suppl_5) ◽  
pp. 146-146
Author(s):  
D. M. Bickhart ◽  
L. Xu ◽  
J. L. Hutchison ◽  
J. B. Cole ◽  
D. J. Null ◽  
...  
Keyword(s):  
Whole Genome Sequencing ◽  
Genetic Markers ◽  
Genome Sequencing ◽  
Copy Number ◽  
Large Scale ◽  
Copy Number Variants ◽  
Whole Genome

Evaluation of Whole Genome Sequencing for outbreak detection of Salmonella enterica serovar Enteritidis

2020 ◽  
Author(s):  
Ainhoa Arrieta-Gisasola ◽  
Aitor Atxaerandio Landa ◽  
Javier Garaizar ◽  
Joseba Bikandi ◽  
José Karkamo ◽  
...  
Keyword(s):  
Whole Genome Sequencing ◽  
Genome Sequencing ◽  
Salmonella Enterica ◽  
Outbreak Detection ◽  
Whole Genome ◽  
Salmonella Enterica Serovar Enteritidis
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