Gene expression in the smut fungus Ustilago esculenta governs swollen gall metamorphosis in Zizania latifolia

2020 ◽  
Vol 143 ◽  
pp. 104107 ◽  
Author(s):  
Zheng-Hong Wang ◽  
Ning Yan ◽  
Xi Luo ◽  
Sai-Sai Guo ◽  
Shu-Qin Xue ◽  
...  
Keyword(s):  
Gene Expression ◽  
Smut Fungus ◽  
Zizania Latifolia ◽  
Ustilago Esculenta

scholarly journals Mating system of Ustilago esculenta and its polymorphism

2018 ◽  
Author(s):  
Syun-Wun Liang ◽  
Yen-Hua Huang ◽  
Jian-Ying Chiu ◽  
Hsin-Wan Tseng ◽  
Jin-Hsing Haung ◽  
...  
Keyword(s):  
Mating System ◽  
Mating Type ◽  
Sex Chromosome ◽  
Infection Process ◽  
Genomic Diversity ◽  
Smut Fungus ◽  
Zizania Latifolia ◽  
Type Locus ◽  
Mating Type Locus ◽  
Ustilago Esculenta

ABSTRACTZizania latifolia Turcz., which is mainly distributed in Asia, has had a long cultivation history as a cereal and vegetable crop. On infection with the smut fungus Ustilago esculenta, Z. latifolia becomes an edible vegetable, water bamboo. Two main cultivars, with a green shell and red shell, are cultivated for commercial production in Taiwan. Previous studies indicated that cultivars of Z. latifolia may be related to infection with U. esculenta isolates. However, related research is limited. The infection process of the corn smut fungus Ustilago maydis is coupled with sexual development and under control of the mating type locus. Thus, we aimed to use knowledge of U. maydis to reveal the mating system of U. esculenta. We collected water bamboo and isolated 145 U. esculenta strains from Taiwan’s major production areas. By using PCR and idiomorph screening among meiotic offspring and field isolates, we identified three idiomorphs of the mating type locus and found no sequence recombination between them. Whole-genome sequencing (Illumina and Pacbio) suggested that the mating system of U. esculenta was bipolar. Mating type locus 1 (MAT-1) was 555,862 bp, and contained 44% repeated sequences. Sequence comparison revealed that U. esculenta MAT-1 shared better conservation with the sex chromosome of U. maydis than U. hordei. These results can be utilized to further explore the genomic diversity of U. esculenta isolates and their application for water bamboo breeding.

Differential gene expression in foxtail millet during interaction with the smut fungus Ustilago crameri

2020 ◽  
Vol 110 ◽  
pp. 101459
Author(s):  
Lihong Hao ◽  
Jing Liu ◽  
Aiying Zhang ◽  
Yansha Han ◽  
Erhu Guo ◽  
...  
Keyword(s):  
Gene Expression ◽  
Differential Gene Expression ◽  
Foxtail Millet ◽  
Smut Fungus ◽  
Differential Gene

scholarly journals Mixed Transcriptome Analysis Revealed the Possible Interaction Mechanisms between Zizania latifolia and Ustilago esculenta Inducing Jiaobai Stem-Gall Formation

2021 ◽  
Vol 22 (22) ◽  
pp. 12258
Author(s):  
Zhi-Ping Zhang ◽  
Si-Xiao Song ◽  
Yan-Cheng Liu ◽  
Xin-Rui Zhu ◽  
Yi-Feng Jiang ◽  
...  
Keyword(s):  
Host Plant ◽  
Expression Patterns ◽  
Gene Expression Pattern ◽  
Hormonal Control ◽  
Transcriptional Analysis ◽  
Gall Formation ◽  
Specific Expression ◽  
Zizania Latifolia ◽  
Stem Gall ◽  
Ustilago Esculenta

The smut fungus Ustilago esculenta infects Zizania latifolia and induces stem expansion to form a unique vegetable named Jiaobai. Although previous studies have demonstrated that hormonal control is essential for triggering stem swelling, the role of hormones synthesized by Z. latifolia and U. esculenta and the underlying molecular mechanism are not yet clear. To study the mechanism that triggers swollen stem formation, we analyzed the gene expression pattern of both interacting organisms during the initial trigger of culm gall formation, at which time the infective hyphae also propagated extensively and penetrated host stem cells. Transcriptional analysis indicated that abundant genes involving fungal pathogenicity and plant resistance were reprogrammed to maintain the subtle balance between the parasite and host. In addition, the expression of genes involved in auxin biosynthesis of U. esculenta obviously decreased during stem swelling, while a large number of genes related to the synthesis, metabolism and signal transduction of hormones of the host plant were stimulated and showed specific expression patterns, particularly, the expression of ZlYUCCA9 (a flavin monooxygenase, the key enzyme in indole-3-acetic acid (IAA) biosynthesis pathway) increased significantly. Simultaneously, the content of IAA increased significantly, while the contents of cytokinin and gibberellin showed the opposite trend. We speculated that auxin produced by the host plant, rather than the fungus, triggers stem swelling. Furthermore, from the differently expressed genes, two candidate Cys2-His2 (C2H2) zinc finger proteins, GME3058_g and GME5963_g, were identified from U. esculenta, which may conduct fungus growth and infection at the initial stage of stem-gall formation.

Cytology and ultrastructure of interactions between Ustilago esculenta and Zizania latifolia

2011 ◽  
Vol 11 (2) ◽  
pp. 499-508 ◽  
Author(s):  
Jing-Ze Zhang ◽  
Fu-Qiang Chu ◽  
De-Ping Guo ◽  
Kevin D. Hyde ◽  
Guang-Lin Xie
Keyword(s):  
Zizania Latifolia ◽  
Ustilago Esculenta

The smut fungus Ustilago esculenta has a bipolar mating system with three idiomorphs larger than 500 kb

2019 ◽  
Vol 126 ◽  
pp. 61-74 ◽  
Author(s):  
Syun-Wun Liang ◽  
Yen-Hua Huang ◽  
Jian-Ying Chiu ◽  
Hsin-Wan Tseng ◽  
Jin-Hsing Huang ◽  
...  
Keyword(s):  
Mating System ◽  
Smut Fungus ◽  
Ustilago Esculenta ◽  
Bipolar Mating System

scholarly journals Makomotindoline from Makomotake, Zizania latifolia infected with Ustilago esculenta

2012 ◽  
Vol 22 (13) ◽  
pp. 4246-4248 ◽  
Author(s):  
Tomohiro Suzuki ◽  
Jae-Hoon Choi ◽  
Takumi Kawaguchi ◽  
Kimiko Yamashita ◽  
Akio Morita ◽  
...  
Keyword(s):  
Zizania Latifolia ◽  
Ustilago Esculenta

The vacuoles containing multivesicular bodies: a new observation in interaction between Ustilago esculenta and Zizania latifolia

2013 ◽  
Vol 138 (1) ◽  
pp. 79-91 ◽  
Author(s):  
Jing-ze Zhang ◽  
Fu-qiang Chu ◽  
De-ping Guo ◽  
Mohammad Reza Ojaghian ◽  
Kevin D Hyde
Keyword(s):  
Multivesicular Bodies ◽  
Zizania Latifolia ◽  
Ustilago Esculenta
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