Characterization of biofilm formation and induction of apoptotic DNA fragmentation by nontypeable Haemophilus influenzae on polarized human airway epithelial cells

2020 ◽  
Vol 141 ◽  
pp. 103985
Author(s):  
Buket Baddal
Keyword(s):  
Epithelial Cells ◽  
Haemophilus Influenzae ◽  
Dna Fragmentation ◽  
Biofilm Formation ◽  
Airway Epithelial Cells ◽  
Airway Epithelial ◽  
Nontypeable Haemophilus Influenzae ◽  
Human Airway ◽  
Human Airway Epithelial Cells

scholarly journals Isolation and characterization of WUPyV in polarized human airway epithelial cells

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Chao Wang ◽  
Tianli Wei ◽  
Yiman Huang ◽  
Qiong Guo ◽  
Zhiping Xie ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Airway Epithelial Cells ◽  
Airway Epithelial ◽  
Human Airway ◽  
Isolation And Characterization ◽  
Human Airway Epithelial Cells

scholarly journals Characterization of the 2009 Pandemic A/Beijing/501/2009 H1N1 Influenza Strain in Human Airway Epithelial Cells and Ferrets

PLoS ONE ◽  
2012 ◽  
Vol 7 (9) ◽  
pp. e46184 ◽  
Author(s):  
Penghui Yang ◽  
Jiejie Deng ◽  
Chenggang Li ◽  
Peirui Zhang ◽  
Li Xing ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
H1n1 Influenza ◽  
Airway Epithelial Cells ◽  
Airway Epithelial ◽  
Human Airway ◽  
Influenza Strain ◽  
2009 H1n1 ◽  
Human Airway Epithelial Cells

scholarly journals The ΔF508-CFTR mutation results in increased biofilm formation byPseudomonas aeruginosaby increasing iron availability

2008 ◽  
Vol 295 (1) ◽  
pp. L25-L37 ◽  
Author(s):  
Sophie Moreau-Marquis ◽  
Jennifer M. Bomberger ◽  
Gregory G. Anderson ◽  
Agnieszka Swiatecka-Urban ◽  
Siying Ye ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Epithelial Cells ◽  
Biofilm Formation ◽  
Airway Epithelial Cells ◽  
Airway Epithelial ◽  
Human Airway ◽  
Abiotic Surfaces ◽  
Δf508 Cftr ◽  
Airway Cells ◽  
Human Airway Epithelial Cells

Enhanced antibiotic resistance of Pseudomonas aeruginosa in the cystic fibrosis (CF) lung is thought to be due to the formation of biofilms. However, there is no information on the antibiotic resistance of P. aeruginosa biofilms grown on human airway epithelial cells or on the effects of airway cells on biofilm formation by P. aeruginosa. Thus we developed a coculture model and report that airway cells increase the resistance of P. aeruginosa to tobramycin (Tb) by >25-fold compared with P. aeruginosa grown on abiotic surfaces. Therefore, the concentration of Tb required to kill P. aeruginosa biofilms on airway cells is 10-fold higher than the concentration achievable in the lungs of CF patients. In addition, CF airway cells expressing ΔF508-CFTR significantly enhanced P. aeruginosa biofilm formation, and ΔF508 rescue with wild-type CFTR reduced biofilm formation. Iron (Fe) content of the airway in CF is elevated, and Fe is known to enhance P. aeruginosa growth. Thus we investigated whether enhanced biofilm formation on ΔF508-CFTR cells was due to increased Fe release by airway cells. We found that airway cells expressing ΔF508-CFTR released more Fe than cells rescued with WT-CFTR. Moreover, Fe chelation reduced biofilm formation on airway cells, whereas Fe supplementation enhanced biofilm formation on airway cells expressing WT-CFTR. These data demonstrate that human airway epithelial cells promote the formation of P. aeruginosa biofilms with a dramatically increased antibiotic resistance. The ΔF508-CFTR mutation enhances biofilm formation, in part, by increasing Fe release into the apical medium.

scholarly journals Infection of Primary Human Bronchial Epithelial Cells by Haemophilus influenzae: Macropinocytosis as a Mechanism of Airway Epithelial Cell Entry

1999 ◽  
Vol 67 (8) ◽  
pp. 4161-4170 ◽  
Author(s):  
Margaret R. Ketterer ◽  
Jian Q. Shao ◽  
Douglas B. Hornick ◽  
Ben Buscher ◽  
Venkata K. Bandi ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Haemophilus Influenzae ◽  
Actin Polymerization ◽  
Microscopic Analysis ◽  
Airway Epithelial Cells ◽  
Airway Epithelial ◽  
Cortical Actin ◽  
Electron Microscopic ◽  
Human Airway ◽  
Human Airway Epithelial Cells

ABSTRACT Nontypeable Haemophilus influenzae is an exclusive human pathogen which infects the respiratory epithelium. We have initiated studies to explore the interaction of the nontypeableH. influenzae strain 2019 with primary human airway epithelial cells by electron and confocal microscopy. Primary human airway cell cultures were established as monolayers on glass collagen-coated coverslips or on semipermeable membranes at an air-fluid interface. Scanning electron microscopy indicated that bacteria adhered to nonciliated cells in the population. The surface of infected cells showed evidence of cytoskeletal rearrangements manifested by microvilli and lamellipodia extending toward and engaging bacteria. Confocal microscopic analysis demonstrated that infection induced actin polymerization with an increase in cortical actin as well as evidence of actin strands around the bacteria. Transmission electron microscopic analysis showed lamellipodia and microvilli surrounding organisms, as well as organisms adherent to the cell surface. These studies also demonstrated the presence of bacteria within vacuoles inside of airway cells. Confocal microscopic studies with Texas red-labeled dextran (molecular weight, 70,000) indicated that H. influenzae cells were entering cells by the process of macropinocytosis. These studies indicate that nontypeable H. influenzae can initiate cytoskeletal rearrangement within human airway epithelium, resulting in internalization of the bacteria within nonciliated human airway epithelial cells by the process of macropinocytosis.

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