AdeB efflux pump gene knockdown by mRNA mediated peptide nucleic acid in multidrug resistance Acinetobacter baumannii

2020 ◽  
Vol 139 ◽  
pp. 103825 ◽  
Author(s):  
Seyed Naser Abdi ◽  
Reza Ghotaslou ◽  
Mohammad Asgharzadeh ◽  
Bahareh Mehramouz ◽  
Alka Hasani ◽  
...  
Keyword(s):  
Multidrug Resistance ◽  
Nucleic Acid ◽  
Acinetobacter Baumannii ◽  
Peptide Nucleic Acid ◽  
Efflux Pump ◽  
Gene Knockdown

scholarly journals Targeted gene knockdown in zebrafish using negatively charged peptide nucleic acid mimics

2003 ◽  
Vol 228 (3) ◽  
pp. 405-413 ◽  
Author(s):  
Karen A. Urtishak ◽  
Michael Choob ◽  
Xiaobing Tian ◽  
Nitzan Sternheim ◽  
William S. Talbot ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Peptide Nucleic Acid ◽  
Gene Knockdown

scholarly journals First Identification and Characterization of an AdeABC-Like Efflux Pump inAcinetobacterGenomospecies 13TU

2011 ◽  
Vol 55 (3) ◽  
pp. 1285-1286 ◽  
Author(s):  
Ignasi Roca ◽  
Paula Espinal ◽  
Sara Martí ◽  
Jordi Vila
Keyword(s):  
Multidrug Resistance ◽  
Acinetobacter Baumannii ◽  
Clinical Isolate ◽  
Nosocomial Infections ◽  
Efflux Pump ◽  
Hospital Setting ◽  
Multidrug Resistant ◽  
Identification Techniques ◽  
Identification And Characterization

ABSTRACTNon-Acinetobacter baumanniispp. are emerging among clinicalAcinetobacterisolates causing nosocomial infections, and some (such as genomospecies 13TU) appear to be multidrug resistant. The prevalence of non-Acinetobacter baumanniispp. in the hospital setting is likely understated due to poor identification techniques. We report the first identification of an AdeABC-type efflux pump in anAcinetobactergenomospecies 13TU clinical isolate, its contribution to multidrug resistance, and the coexistence of three Ade-type efflux pumps in this strain.

scholarly journals CraA, a Major Facilitator Superfamily Efflux Pump Associated with Chloramphenicol Resistance in Acinetobacter baumannii

2009 ◽  
Vol 53 (9) ◽  
pp. 4013-4014 ◽  
Author(s):  
I. Roca ◽  
S. Marti ◽  
P. Espinal ◽  
P. Martínez ◽  
I. Gibert ◽  
...  
Keyword(s):  
Multidrug Resistance ◽  
Acinetobacter Baumannii ◽  
Antimicrobial Agents ◽  
Efflux Pump ◽  
Major Facilitator Superfamily ◽  
Intrinsic Resistance ◽  
Chloramphenicol Resistance ◽  
Hospital Acquired Infections ◽  
Major Facilitator ◽  
Hospital Acquired

ABSTRACT Acinetobacter baumannii has been increasingly associated with hospital-acquired infections, and the presence of multidrug resistance strains is of great concern to clinicians. A. baumannii is thought to possess a great deal of intrinsic resistance to several antimicrobial agents, including chloramphenicol, although the mechanisms involved in such resistance are not well understood. In this work, we have identified a major facilitator superfamily efflux pump present in most A. baumannii strains, displaying strong substrate specificity toward chloramphenicol.

scholarly journals Genome Sequence of a Clinical Strain of Acinetobacter baumannii Belonging to the ST79/PFGE-HUI-1 Clone Lacking the AdeABC (Resistance-Nodulation-Cell Division-Type) Efflux Pump

2016 ◽  
Vol 4 (5) ◽  
Author(s):  
M. López ◽  
L. Álvarez-Fraga ◽  
E. Gato ◽  
L. Blasco ◽  
M. Poza ◽  
...  
Keyword(s):  
Multidrug Resistance ◽  
Cell Division ◽  
Acinetobacter Baumannii ◽  
Genome Sequence ◽  
Efflux Pump ◽  
Clinical Strain ◽  
Genetic Characteristics ◽  
Clinical Strains ◽  
Chromosomal Genes

Increased expression of chromosomal genes for resistance-nodulation-cell division-type efflux systems plays a major role in the multidrug resistance of Acinetobacter baumannii . Little is known about the genetic characteristics of clinical strains of Acinetobacter baumannii lacking the AdeABC pump. In this study, we sequenced the genome of clinical strain Ab421 GEIH-2010 (belonging to clone ST79/PFGE-HUI-1 from the GEIH-REIPI Ab. 2010 project) which lacks this efflux pump.

scholarly journals Screening antibiotics using an Hoechst 33342 dye-accumulation assay to detect efflux activity in Acinetobacter baumannii clinical isolates

2018 ◽  
Vol 11 (4) ◽  
pp. 371-378 ◽  
Author(s):  
In-Sun Choi ◽  
Choon-Mee Kim ◽  
Sook-Jin Jang
Keyword(s):  
Multidrug Resistance ◽  
Acinetobacter Baumannii ◽  
Efflux Pump ◽  
Multidrug Resistant ◽  
Clinical Isolates ◽  
Drug Efflux ◽  
Hoechst 33342 ◽  
Efflux Pump Inhibitor ◽  
Significant Difference

AbstractBackgroundUnderstanding the contribution of efflux pumps to the resistance of antibiotics is useful when considering strategies for antimicrobial therapy.ObjectivesTo assess the role of efflux activity on the resistance of antibiotics commonly used in hospitals.MethodsWe analyzed the efflux activity of 120 clinical isolates of Acinetobacter baumannii using an Hoechst 33342 (H33342) dye-accumulation assay. We compared the indicators for efflux activity of susceptible and non-susceptible groups of each of 16 tested antibiotics. To determine the role of efflux activity on resistance to an antibiotic, we used 3 criteria based on the results of the H33342-accumulation assay.ResultsThe evaluation suggests that efflux activity contributed to resistance to the following 11 antibiotics: cefepime, cefotaxime, ceftazidime, ciprofloxacin, gentamicin, imipenem, meropenem, piperacillin, piperacillin/tazobactam, ticarcillin/ clavulanic acid, and tigecycline. However, ampicillin/sulbactam, minocycline, and trimethoprim/sulfamethoxazole did not meet the criteria, suggesting resistance may not be mediated by efflux activity. A significant difference in efflux activity was observed between bacteria belonging to the multidrug-resistant Acinetobacter baumannii (MDRAB) group and those belonging to the non-MDRAB group.ConclusionsEfflux activity may contribute to multidrug resistance and particularly resistance to numerous antibiotics used in hospitals. These antibiotics would be good candidates for combination therapeutic regimens consisting of an antibiotic and an efflux pump inhibitor as an adjuvant to combat drug efflux.

scholarly journals Active efflux pump adeB is involved in multidrug resistance of Acinetobacter baumannii induced by antibacterial agents

2017 ◽  
Vol 13 (4) ◽  
pp. 1538-1546 ◽  
Author(s):  
Tingting Zhang ◽  
Min Wang ◽  
Yixin Xie ◽  
Xianping Li ◽  
Zhihui Dong ◽  
...  
Keyword(s):  
Multidrug Resistance ◽  
Acinetobacter Baumannii ◽  
Antibacterial Agents ◽  
Efflux Pump ◽  
Active Efflux ◽  
Active Efflux Pump
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