‘Biofilm Clippers’- enzyme formulation for bovine mastitic biofilm therapy

2019 ◽  
Vol 137 ◽  
pp. 103740
Author(s):  
Muthu Thiruvengadam ◽  
Baskar Venkidasamy ◽  
Priyadharshini Karuppasamy ◽  
Raman Muthusamy ◽  
Shivraj Hariram Nile ◽  
...  
Keyword(s):  
Enzyme Formulation

scholarly journals Comparison of Techniques to Control Ice Nucleation during Lyophilization

Processes ◽  
2020 ◽  
Vol 8 (11) ◽  
pp. 1439
Author(s):  
Jacob Luoma ◽  
Erika Ingham ◽  
Carmen Lema Martinez ◽  
Andrea Allmendinger
Keyword(s):  
Drug Product ◽  
Quality Attributes ◽  
Ice Nucleation ◽  
Residual Moisture ◽  
Critical Quality Attributes ◽  
Stability Behavior ◽  
Drug Products ◽  
Ice Fog ◽  
Comparison Of Techniques ◽  
Enzyme Formulation

Controlling ice nucleation during lyophilization of parenteral drug products increases the homogeneity of critical quality attributes, such as residual moisture, across drug product batches and shortens lyophilization cycle time. In the present study, we compare three mechanistically different techniques to control ice nucleation during the freezing step of lyophilization, which are referred to as “depressurization”, “partial vacuum”, and “ice fog” techniques. The techniques are compared with respect to their operational limitations and challenges. Installation considerations are also discussed. Using the aforementioned nucleation techniques, we investigated a monoclonal antibody formulation and an enzyme formulation at different protein concentrations using feasible nucleation temperatures and different vial formats and fill volumes. Samples were compared for solid state properties and other critical quality attributes on stability. When nucleated at the same temperature, the three techniques produced products with the same quality attributes and stability behavior. Under conditions resulting in micro-collapse, stability behavior can be different. We found that each technology had considerations for achieving robust nucleation. The present comparison may serve as guidance in selecting a nucleation method.

scholarly journals In Silico and In Vitro Tailoring of a Chitosan Nanoformulation of a Human Metabolic Enzyme

Pharmaceutics ◽  
2021 ◽  
Vol 13 (3) ◽  
pp. 329
Author(s):  
Paulo R. Lino ◽  
João Leandro ◽  
Mariana Amaro ◽  
Lídia M. D. Gonçalves ◽  
Paula Leandro ◽  
...  
Keyword(s):  
In Silico ◽  
Rational Design ◽  
Phenylalanine Hydroxylase ◽  
Metabolic Enzyme ◽  
Large Set ◽  
New Therapeutic Approaches ◽  
Catalytic Function ◽  
And Function ◽  
Enzyme Formulation

Enzyme nanoencapsulation holds an enormous potential to develop new therapeutic approaches to a large set of human pathologies including cancer, infectious diseases and inherited metabolic disorders. However, enzyme formulation has been limited by the need to maintain the catalytic function, which is governed by protein conformation. Herein we report the rational design of a delivery system based on chitosan for effective encapsulation of a functionally and structurally complex human metabolic enzyme through ionic gelation with tripolyphosphate. The rationale was to use a mild methodology to entrap the multimeric multidomain 200 kDa human phenylalanine hydroxylase (hPAH) in a polyol-like matrix that would allow an efficient maintenance of protein structure and function, avoiding formulation stress conditions. Through an in silico and in vitro based development, the particulate system was optimized with modulation of nanomaterials protonation status, polymer, counterion and protein ratios, taking into account particle size, polydispersity index, surface charge, particle yield production, protein free energy of folding, electrostatic surface potential, charge, encapsulation efficiency, loading capacity and transmission electron microscopy morphology. Evaluation of the thermal stability, substrate binding profile, relative enzymatic activity, and substrate activation ratio of the encapsulated hPAH suggests that the formulation procedure does not affect protein stability, allowing an effective maintenance of hPAH biological function. Hence, this study provides an important framework for an enzyme formulation process.

Dissolving a Nutrition Clog With a New Pancreatic Enzyme Formulation

2013 ◽  
Vol 28 (3) ◽  
pp. 410-412 ◽  
Author(s):  
Mark G. Klang ◽  
Unnati D. Gandhi ◽  
Olga Mironova
Keyword(s):  
Pancreatic Enzyme ◽  
Enzyme Formulation

scholarly journals Production of heterologous cutinases by E. coli and improved enzyme formulation for application on plastic degradation

2013 ◽  
Vol 16 (5) ◽  
Author(s):  
Daniela Gomes ◽  
Teresa Matamá ◽  
Artur Cavaco-Paulo ◽  
Galba Takaki ◽  
Alexandra Salgueiro
Keyword(s):  
E Coli ◽  
Plastic Degradation ◽  
Enzyme Formulation
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