Lack of correlation of virulence gene profiles of Staphylococcus aureus bacteremia isolates with mortality

2019 ◽  
Vol 133 ◽  
pp. 103543 ◽  
Author(s):  
Kyung-Hwa Park ◽  
Kerryl E. Greenwood-Quaintance ◽  
Scott A. Cunningham ◽  
Govindarajan Rajagopalan ◽  
Nicholas Chia ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Virulence Gene ◽  
Staphylococcus Aureus Bacteremia ◽  
Gene Profiles

scholarly journals Molecular characteristics and virulence gene profiles of Staphylococcus aureus isolates in Hainan, China

2019 ◽  
Author(s):  
Xuehan Li ◽  
Kai Xu ◽  
Chenglin Li ◽  
Tao Huang ◽  
Yirong Li
Keyword(s):  
Staphylococcus Aureus ◽  
Antibiotic Resistance ◽  
Virulence Genes ◽  
Sccmec Type ◽  
Mainland China ◽  
Virulence Gene ◽  
Molecular Characteristics ◽  
Spa Typing ◽  
Gene Profiles ◽  
High Prevalence

Abstract Background: There have been no reports regarding the molecular characteristics, virulence features, and antibiotic resistance profiles of Staphylococcus aureus (S. aureus) from Hainan, the southernmost province of China. Methods: 227 S. aureus isolates, consisting of 76 methicillin-resistant S. aureus (MRSA) and 151 methicillin-susceptible S. aureus (MSSA), were collected in 2013-2014 and 2018-2019 in Hainan, and investigated for their molecular characteristics, virulence genes, and antibiotic resistance profiles. Results: Thirty-four sequence types (STs) and 79 spa types were identified based on multilocus sequence typing (MLST) and spa typing, respectively. ST398 (14.1%, 32/227) was found to be the most prevalent, and moreover, the prevalence of ST398-MSSA increased significantly from 2013-2014 (5.5%, 5/91) to 2018-2019 (18.4%, 25/136). Seventy-six MRSA isolates were subject to staphylococcus chromosomal cassette mec (SCCmec) typing. SCCmec-IVa was the predominant SCCmec type, and specifically ST45-SCCmec IVa, an infrequent type in mainland China, was predominant in S. aureus from Hainan. Eleven virulence genes, including the Panton-Valentine leukocidin (pvl) and eta, were determined, and the positive rates of eta and pvl were found to be 57.3% and 47.6%. Such high prevalence has never been seen in mainland China before.

Virulence gene profiles inStaphylococcus aureusisolated from cows with subclinical mastitis in eastern Poland

2016 ◽  
Vol 83 (2) ◽  
pp. 228-235 ◽  
Author(s):  
Barbara Kot ◽  
Piotr Szweda ◽  
Aneta Frankowska-Maciejewska ◽  
Małgorzata Piechota ◽  
Katarzyna Wolska
Keyword(s):  
Staphylococcus Aureus ◽  
Serine Protease ◽  
Virulence Genes ◽  
Bovine Mastitis ◽  
Virulence Gene ◽  
Subclinical Mastitis ◽  
Staph Aureus ◽  
Gene Profiles ◽  
Enterotoxin Genes ◽  
Genes Encoding

Staphylococcus aureusis arguably the most important pathogen involved in bovine mastitis. The aim of this study was to determine the virulence gene profiles of 124Staph. aureusisolates from subclinical mastitis in cows in eastern Poland. The presence of 30 virulence genes encoding adhesins, proteases and superantigenic toxins was investigated by PCR. The 17 different combinations of adhesin genes were identified. Occurrence ofeno(91·1%) andfib(82·3%) genes was found to be common. The frequency of other adhesion genesfnbA, fnbB, ebpswere 14·5, 50, 25%, respectively, and forcnaandbbpwere 1·6%. TheetAandetDgenes, encoding exfoliative toxins, were present in genomes of 5·6 and 8·9% isolates, respectively. ThesplAandsspA, encoding serine protease, were detected in above 90% isolates. The most frequent enterotoxin genes weresei(21%),sem(19·4%),sen(19·4%),seg(18·5%) andseo(13·7%). Thetstgene was harboured by 2·4% isolates. The 19 combinations of the superantigenic toxin genes were obtained and found in 35·5% of isolates. Three of them (seg, sei, sem, sen, seo; sec, seg, sei, sem, sen, seoandseg, sei, sem, sen) were the most frequent and found in 16·1% of the isolates. The most common virulotype, present in 17·7% of the isolates, wasfib, eno, fnbB, splA, splE, sspA. The results indicate the variation in the presence of virulence genes inStaph. aureusisolates and considerable diversity of isolates that are able to cause mastitis in cows.

scholarly journals Genotypic Characterization of Clinical Isolates of Staphylococcus aureus from Pakistan

Pathogens ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 918
Author(s):  
Saeed Khan ◽  
Bernard S. Marasa ◽  
Kidon Sung ◽  
Mohamed Nawaz
Keyword(s):  
Staphylococcus Aureus ◽  
Screening Program ◽  
Sccmec Type ◽  
Virulence Gene ◽  
Multidrug Resistant ◽  
Spa Typing ◽  
Evolution And Development ◽  
Gene Profiles ◽  
Spa Types

In this study, we compared pulsed-field gel electrophoretic (PFGE), multilocus sequence typing (MLST), Staphylococcal cassette chromosome mec (SCCmec), spa typing, and virulence gene profiles of 19 Panton–Valentine leucocidin (PVL)-positive, multidrug-, and methicillin-resistant clinical Staphylococcus aureus (MRSA) isolates obtained from a hospital intensive care unit in Pakistan. The isolates exhibited 10 pulsotypes, contained eight adhesin genes (bbp, clfA, clfB, cna, fnbA, fnbB, map-eap, and spa), 10 toxin genes (hla, hlb, hld, hlg, pvl, sed, see, seg, seh, and tst), and two other virulence genes (cfb, v8) that were commonly present in all isolates. The spa-typing indicated seven known spa types (t030, t064, t138, t314, t987, t1509, and t5414) and three novel spa types. MLST analysis indicated eight ST types (ST8, ST15, ST30, ST239, ST291, ST503, ST772, and ST1413). All isolates belonged to the agr group 1. Most of the isolates possessed SCCmec type III, but some isolates had it in combination with types SCCmec IV and V. The presence of multidrug-resistant MRSA isolates in Pakistan indicates poor hygienic conditions, overuse of antibiotics, and a lack of rational antibiotic therapy that have led to the evolution and development of hypervirulent MRSA clones. The study warrants development of a robust epidemiological screening program and adoption of effective measures to stop their spread in hospitals and the community.

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