RNA chaperone hfq mediates persistence to multiple antibiotics in Aeromonas veronii

2019 ◽  
Vol 132 ◽  
pp. 124-128 ◽  
Author(s):  
Lin Zhang ◽  
Wenjing Yu ◽  
Yanqiong Tang ◽  
Hong Li ◽  
Xiang Ma ◽  
...  
Keyword(s):  
Rna Chaperone ◽  
Aeromonas Veronii ◽  
Multiple Antibiotics

scholarly journals The RNA Chaperone Hfq Participates in Persistence to Multiple Antibiotics in the Fish Pathogen Yersinia ruckeri

2021 ◽  
Vol 9 (7) ◽  
pp. 1404
Author(s):  
Iván L. Calderón ◽  
María José Barros ◽  
Fernanda Montt ◽  
Fernando Gil ◽  
Juan A. Fuentes ◽  
...  
Keyword(s):  
Growth Curves ◽  
Polymyxin B ◽  
Yersinia Ruckeri ◽  
Knockout Mutant ◽  
Persister Cells ◽  
Rna Chaperone ◽  
Time Kill ◽  
Sublethal Concentrations ◽  
Multiple Antibiotics

Yersinia ruckeri causes outbreaks of enteric redmouth disease in salmon aquaculture all over the world. The transient antibiotic tolerance exhibited by bacterial persisters is commonly thought to be responsible for outbreaks; however, the molecular factors underlying this behavior have not been explored in Y. ruckeri. In this study, we investigated the participation of the RNA chaperone Hfq from Y. ruckeri in antibiotic persistence. Cultures of the hfq-knockout mutant (Δhfq) exhibited faster replication, increased ATP levels and a more reductive environment than the wild type. The growth curves of bacteria exposed to sublethal concentrations of ampicillin, oxolinic acid, ciprofloxacin and polymyxin B revealed a greater susceptibility for the Δhfq strain. The time-kill curves of bacteria treated with the antibiotics mentioned above and florfenicol, using inoculums from exponential, stationary and biofilm cultures, demonstrated that the Δhfq strain has significant defects in persister cells production. To shed more light on the role of Hfq in antibiotic persistence, we analyzed its dependence on the (p)ppGpp synthetase RelA by determining the persister cells production in the absence of the relA gene. The ΔrelA and ΔrelAΔhfq strains displayed similar defects in persister cells formation, but higher than Δhfq strain. Similarly, stationary cultures of the ΔrelA and ΔrelAΔhfq strains exhibited comparable levels of ATP but higher than that of the Δhfq strain, indicating that relA is epistatic over hfq. Taken together, our findings provide valuable information on antibiotic persistence in Y. ruckeri, shedding light on the participation of Hfq in the persistence phenomenon.

scholarly journals Arginine methylation enhances the RNA chaperone activity of the West Nile virus host factor AUF1 p45

RNA ◽  
2016 ◽  
Vol 22 (10) ◽  
pp. 1574-1591 ◽  
Author(s):  
Susann Friedrich ◽  
Tobias Schmidt ◽  
Angelika Schierhorn ◽  
Hauke Lilie ◽  
Grit Szczepankiewicz ◽  
...  
Keyword(s):  
West Nile Virus ◽  
Arginine Methylation ◽  
Host Factor ◽  
Chaperone Activity ◽  
The West ◽  
West Nile ◽  
Rna Chaperone

scholarly journals Arabidopsis Disulfide Reductase, Trx-h2, Functions as an RNA Chaperone under Cold Stress

2021 ◽  
Vol 11 (15) ◽  
pp. 6865
Author(s):  
Eun Seon Lee ◽  
Joung Hun Park ◽  
Seong Dong Wi ◽  
Ho Byoung Chae ◽  
Seol Ki Paeng ◽  
...  
Keyword(s):  
Cold Stress ◽  
Wild Type ◽  
Rna Chaperone ◽  
E Coli ◽  
Cold Sensitive ◽  
Thioredoxin H ◽  
Functional Rnas

The thioredoxin-h (Trx-h) family of Arabidopsis thaliana comprises cytosolic disulfide reductases. However, the physiological function of Trx-h2, which contains an additional 19 amino acids at its N-terminus, remains unclear. In this study, we investigated the molecular function of Trx-h2 both in vitro and in vivo and found that Arabidopsis Trx-h2 overexpression (Trx-h2OE) lines showed significantly longer roots than wild-type plants under cold stress. Therefore, we further investigated the role of Trx-h2 under cold stress. Our results revealed that Trx-h2 functions as an RNA chaperone by melting misfolded and non-functional RNAs, and by facilitating their correct folding into active forms with native conformation. We showed that Trx-h2 binds to and efficiently melts nucleic acids (ssDNA, dsDNA, and RNA), and facilitates the export of mRNAs from the nucleus to the cytoplasm under cold stress. Moreover, overexpression of Trx-h2 increased the survival rate of the cold-sensitive E. coli BX04 cells under low temperature. Thus, our data show that Trx-h2 performs function as an RNA chaperone under cold stress, thus increasing plant cold tolerance.

scholarly journals Network-based analysis of virulence factors for uncovering Aeromonas veronii pathogenesis

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Hong Li ◽  
Xiang Ma ◽  
Yanqiong Tang ◽  
Dan Wang ◽  
Ziding Zhang ◽  
...  
Keyword(s):  
Virulence Factors ◽  
Bacterial Pathogen ◽  
Signal Transduction Pathway ◽  
Immune Defense ◽  
Ppi Network ◽  
Host Proteins ◽  
Homologous Proteins ◽  
Aeromonas Veronii ◽  
Regulatory Systems ◽  
Network Modules

Abstract Background Aeromonas veronii is a bacterial pathogen in aquaculture, which produces virulence factors to enable it colonize and evade host immune defense. Given that experimental verification of virulence factors is time-consuming and laborious, few virulence factors have been characterized. Moreover, most studies have only focused on single virulence factors, resulting in biased interpretation of the pathogenesis of A. veronii. Results In this study, a PPI network at genome-wide scale for A. veronii was first constructed followed by prediction and mapping of virulence factors on the network. When topological characteristics were analyzed, the virulence factors had higher degree and betweenness centrality than other proteins in the network. In particular, the virulence factors tended to interact with each other and were enriched in two network modules. One of the modules mainly consisted of histidine kinases, response regulators, diguanylate cyclases and phosphodiesterases, which play important roles in two-component regulatory systems and the synthesis and degradation of cyclic-diGMP. Construction of the interspecies PPI network between A. veronii and its host Oreochromis niloticus revealed that the virulence factors interacted with homologous proteins in the host. Finally, the structures and interacting sites of the virulence factors during interaction with host proteins were predicted. Conclusions The findings here indicate that the virulence factors probably regulate the virulence of A. veronii by involving in signal transduction pathway and manipulate host biological processes by mimicking and binding competitively to host proteins. Our results give more insight into the pathogenesis of A. veronii and provides important information for designing targeted antibacterial drugs.

scholarly journals Enhancement of the production of L-glutaminase, an anticancer enzyme, from Aeromonas veronii by adaptive and induced mutation techniques

PLoS ONE ◽  
2017 ◽  
Vol 12 (8) ◽  
pp. e0181745 ◽  
Author(s):  
S. Aravinth Vijay Jesuraj ◽  
Md. Moklesur Rahman Sarker ◽  
Long Chiau Ming ◽  
S. Marylin Jeya Praya ◽  
M. Ravikumar ◽  
...  
Keyword(s):  
Induced Mutation ◽  
Aeromonas Veronii

scholarly journals Structural Studies of the Lipopolysaccharide of Aeromonas veronii bv. sobria Strain K133 which Represents New Provisional Serogroup PGO1 Prevailing among Mesophilic Aeromonads on Polish Fish Farms

2021 ◽  
Vol 22 (8) ◽  
pp. 4272
Author(s):  
Katarzyna Dworaczek ◽  
Maria Kurzylewska ◽  
Magdalena Laban ◽  
Dominika Drzewiecka ◽  
Agnieszka Pękala-Safińska ◽  
...  
Keyword(s):  
Epidemiological Data ◽  
13C Nmr Spectroscopy ◽  
Freshwater Species ◽  
Fish Farms ◽  
1H And 13C Nmr ◽  
Aeromonas Veronii ◽  
Specific Polysaccharide ◽  
O Antigens ◽  
Unique Composition

In the present work, we performed immunochemical studies of LPS, especially the O-specific polysaccharide (O-PS) of Aeromonas veronii bv. sobria strain K133, which was isolated from the kidney of carp (Cyprinus carpio L.) during an outbreak of motile aeromonad infection/motile aeromonad septicemia (MAI/MAS) on a Polish fish farm. The structural characterization of the O-PS, which was obtained by mild acid degradation of the LPS, was performed with chemical methods, MALDI-TOF mass spectrometry, and 1H and 13C NMR spectroscopy. It was revealed that the O-PS has a unique composition of a linear tetrasaccharide repeating unit and contains a rarely occurring sugar 2,4-diamino-2,4,6-trideoxy-D-glucose (bacillosamine), which may determine the specificity of the serogroup. Western blotting and ELISA confirmed that A. veronii bv. sobria strain K133 belongs to the new serogroup PGO1, which is one of the most commonly represented immunotypes among carp and trout isolates of Aeromonas sp. in Polish aquacultures. Considering the increase in the MAI/MAS incidences and their impact on freshwater species, also with economic importance, and in the absence of an effective immunoprophylaxis, studies of the Aeromonas O-antigens are relevant in the light of epidemiological data and monitoring emergent pathogens representing unknown antigenic variants and serotypes.

scholarly journals Characterization of Virulence Properties of Aeromonas veronii Isolated from Diseased Gibel Carp (Carassius gibelio)

2016 ◽  
Vol 17 (4) ◽  
pp. 496 ◽  
Author(s):  
Jingjing Sun ◽  
Xiaojun Zhang ◽  
Xiaojian Gao ◽  
Qun Jiang ◽  
Yi Wen ◽  
...  
Keyword(s):  
Gibel Carp ◽  
Aeromonas Veronii ◽  
Carassius Gibelio ◽  
Virulence Properties
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