Immunogenic reactivity of recombinant PKF and AbOmpA proteins as serum resistance factors against sepsis of Acinetobacter baumannii

2019 ◽  
Vol 131 ◽  
pp. 9-14 ◽  
Author(s):  
Negin Bolourchi ◽  
Fereshteh Shahcheraghi ◽  
Armaghan Soltani Shirazi ◽  
Alireza Janani ◽  
Fariborz Bahrami ◽  
...  
Keyword(s):  
Acinetobacter Baumannii ◽  
Serum Resistance ◽  
Resistance Factors

scholarly journals Variability in carbapenemase activity of intrinsic OxaAb (OXA-51-like) β-lactamase enzymes in Acinetobacter baumannii

2020 ◽  
Author(s):  
Yuiko Takebayashi ◽  
Jacqueline Findlay ◽  
Kate J Heesom ◽  
Philip J Warburton ◽  
Matthew B Avison ◽  
...  
Keyword(s):  
Molecular Evolution ◽  
Acinetobacter Baumannii ◽  
Active Site ◽  
Carbapenem Resistance ◽  
Clinical Isolates ◽  
Resistance Factors ◽  
Resistance Determinants ◽  
Evolution Analysis ◽  
Antimicrobial Susceptibility Tests ◽  
Susceptibility Tests

Abstract Objectives To measure the variability in carbapenem susceptibility conferred by different OxaAb variants, characterize the molecular evolution of oxaAb and elucidate the contribution of OxaAb and other possible carbapenem resistance factors in the clinical isolates using WGS and LC–MS/MS. Methods Antimicrobial susceptibility tests were performed on 10 clinical Acinetobacter baumannii isolates. Carbapenem MICs were evaluated for all oxaAb variants cloned into A. baumannii CIP70.10 and BM4547, with and without their natural promoters. Molecular evolution analysis of the oxaAb variants was performed using FastTree and SplitsTree4. Resistance determinants were studied in the clinical isolates using WGS and LC–MS/MS. Results Only the OxaAb variants with I129L and L167V substitutions, OxaAb(82), OxaAb(83), OxaAb(107) and OxaAb(110) increased carbapenem MICs when expressed in susceptible A. baumannii backgrounds without an upstream IS element. Carbapenem resistance was conferred with the addition of their natural upstream ISAba1 promoter. LC–MS/MS analysis on the original clinical isolates confirmed overexpression of the four I129L and L167V variants. No other differences in expression levels of proteins commonly associated with carbapenem resistance were detected. Conclusions Elevated carbapenem MICs were observed by expression of OxaAb variants carrying clinically prevalent substitutions I129L and L167V. To drive carbapenem resistance, these variants required overexpression by their upstream ISAba1 promoter. This study clearly demonstrates that a combination of IS-driven overexpression of oxaAb and the presence of particular amino acid substitutions in the active site to improve carbapenem capture is key in conferring carbapenem resistance in A. baumannii and other mechanisms are not required.

scholarly journals Comparative in silico analyses of proteins involved in serum resistance as promising vaccine candidates against Acinetobacter baumannii

2017 ◽  
Vol 4 (3) ◽  
pp. 64-71 ◽  
Author(s):  
A Soltani Shirazi ◽  
O Azizi ◽  
N Bolourchi ◽  
Sh Aghamohammad ◽  
F Badmasti ◽  
...  
Keyword(s):  
Acinetobacter Baumannii ◽  
In Silico ◽  
Serum Resistance ◽  
Vaccine Candidates ◽  
In Silico Analyses

Adherence ability and serum resistance of different hospital clusters of Acinetobacter baumannii

2021 ◽  
Author(s):  
Ana Paula Uber ◽  
Giselle Fukita Viana ◽  
Fernanda Gomes Lodi ◽  
Márcia Maria dos Anjos Szczerepa ◽  
Floristher Elaine Carrara‐Marroni ◽  
...  
Keyword(s):  
Acinetobacter Baumannii ◽  
Serum Resistance ◽  
Adherence Ability
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