Fungal epidemiology in cystic fibrosis patients with a special focus on Scedosporium species complex

2019 ◽  
Vol 129 ◽  
pp. 168-175 ◽  
Author(s):  
Mohammad T. Hedayati ◽  
Mahin Tavakoli ◽  
Maedeh Maleki ◽  
Somaye Heidari ◽  
Vida Mortezaee ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Species Complex ◽  
Special Focus ◽  
Scedosporium Species

scholarly journals Long-Term Rasamsonia argillacea Complex Species Colonization Revealed by PCR Amplification of Repetitive DNA Sequences in Cystic Fibrosis Patients

2016 ◽  
Vol 54 (11) ◽  
pp. 2804-2812 ◽  
Author(s):  
Abdelmounaim Mouhajir ◽  
Olivier Matray ◽  
Sandrine Giraud ◽  
Laurent Mély ◽  
Christophe Marguet ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Repetitive Dna ◽  
Dna Sequences ◽  
Species Complex ◽  
Pcr Amplification ◽  
Patient Specific ◽  
Molecular Evidence ◽  
Repetitive Dna Sequences ◽  
Complex Species ◽  
Content Type

The aim of this work was to document molecular epidemiology of Rasamsonia argillacea species complex isolates from cystic fibrosis (CF) patients. In this work, 116 isolates belonging to this species complex and collected from 26 CF patients and one patient with chronic granulomatous disease were characterized using PCR amplification assays of repetitive DNA sequences and electrophoretic separation of amplicons (rep-PCR). Data revealed a clustering consistent with molecular species identification. A single species was recovered from most patients. Rasamsonia aegroticola was the most common species, followed by R. argillacea sensu stricto and R. piperina , while R. eburnea was not identified. Of 29 genotypes, 7 were shared by distinct patients while 22 were patient specific. In each clinical sample, most isolates exhibited an identical genotype. Genotyping of isolates recovered from sequential samples from the same patient confirmed the capability of R. aegroticola and R. argillacea isolates to chronically colonize the airways. A unique genotype was recovered from two siblings during a 6-month period. In the other cases, a largely dominant genotype was detected. Present results which support the use of rep-PCR for both identification and genotyping for the R. argillacea species complex provide the first molecular evidence of chronic airway colonization by these fungi in CF patients.

scholarly journals Reverse line blot hybridisation screening of Pseudallescheria/Scedosporium species in patients with cystic fibrosis

Mycoses ◽  
2011 ◽  
Vol 54 ◽  
pp. 5-11 ◽  
Author(s):  
Q. Lu ◽  
A. H. G. Gerrits van den Ende ◽  
G. S. de Hoog ◽  
R. Li ◽  
I. Accoceberry ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Reverse Line Blot ◽  
Blot Hybridisation ◽  
Scedosporium Species

scholarly journals Taxonomic review of Australian Mecyclothorax Sharp (Coleoptera, Carabidae, Moriomorphini) with special emphasis on the M. lophoides (Chaudoir) species complex

2018 ◽  
Vol 65 (2) ◽  
pp. 177-224 ◽  
Author(s):  
James K. Liebherr
Keyword(s):  
Western Australia ◽  
Species Complex ◽  
Sister Group ◽  
Late Eocene ◽  
Sister Species ◽  
Sister Group Relationship ◽  
Special Focus ◽  
Species Pair ◽  
Spermathecal Duct ◽  
Biogeographic History

The Australian fauna ofMecyclothoraxSharp (Coleoptera: Carabidae: Moriomorphini) is reviewed, with special focus on species assigned to the monophyletic subgenus Eucyclothorax Liebherr:M.isolatus,sp. n.from Western Australia,M.mooreiBaehr,M.punctatus(Sloane),M.curtus(Sloane),M.blackburni(Sloane);M.eyrensis(Blackburn);M.peryphoides(Blackburn);M.darlingtoni,sp. n.from Queensland;M.jameswalkeri,sp. n.from Western Australia;M.lophoides(Chaudoir); andM.cordicollis(Sloane). The last six species listed above–theM.lophoidesspecies complex–have been the source of long-term confusion for taxonomists, with male genitalic characters providing trouble-free species circumscription. One new subspecies,M.lewisensisestriatus,subsp. n.from Queensland is added to the seven previously described taxa of the monophyletic subgenus Qecyclothorax Liebherr. The balance of the fauna consists of four species in the subgenus Mecyclothorax:1and2, the sister-species pairM.lateralis(Castelnau) andM.minutus(Castelnau);3,M.ambiguus(Erichson); and4,M.punctipennis(MacLeay).Mecyclothoraxfortis(Blackburn),syn. n., is newly synonymized withM.minutus.MecyclothoraxovalisSloane is recombined asNeonomiusovalis(Sloane),comb. n., and a neotype is designated to replace the destroyed holotype. Phylogenetic relationships for the AustralianMecyclothoraxare proposed based on information from 68 terminal taxa and 139 morphological characters. The biogeographic history of AustralianMecyclothoraxis deduced based the sister-group relationship betweenMecyclothoraxand theAmblytelus-related genera, with both groups hypothesized to have originated during the late Eocene. Diversification withinMecyclothoraxhas occurred since then in montane rainforests of tropical Queensland, temperate forest biomes of the southwest and southeast, and in grasslands and riparian habitats adjacent and inland from those forests. Several species presently occupy interior desert regions, though no sister species mutually occupy such climatically harsh habitats. TheM.lophoidesspecies complex exhibits profound male genitalic diversification within the context of conserved external anatomy. This disparity is investigated with regard to the functional interaction of the male internal sac flagellum and female spermathecal duct. Though limited association of flagellar and spermathecal duct configurations can be documented, several factors complicate proposing a general evolutionary mechanism for the observed data. These include:1, the occurrence of derived, elongate spermathecal ducts in three species, two of which exhibit very long male flagella, whereas males of the third exhibit a very short flagellum; and2, a highly derived and exaggerated male flagellar configuration shared across a sister-species pair even though the two species can be robustly diagnosed using external anatomical characters, other significant genitalic differences involving male parameral setation, and biogeographic allopatry associated with differential occupation of desert versus forest biomes.

scholarly journals Updated status of whiteflies (Hemiptera: Aleyrodidae) in Jordan with emphasis on the Bemisia tabaci species complex

2021 ◽  
Vol 47 (1) ◽  
pp. 1-9
Author(s):  
Ihab Ghabeish ◽  
Mais Sweiss ◽  
Ghandi Anfoka
Keyword(s):  
Bemisia Tabaci ◽  
Species Complex ◽  
Diagnostic Techniques ◽  
Morphological Identification ◽  
Special Focus ◽  
Plant Pests ◽  
Putative Species ◽  
Geographical Regions ◽  
The Status ◽  
Mtcoi Gene

Whiteflies are economically important plant pests that cause damage to crops worldwide. This study aimed to update the status of whiteflies in Jordan by combining the classical morphological identification and the DNA markers using the mitochondrial cytochrome oxidase I (mtCOI) gene. Over the course of three consecutive years, 111 whiteflies were collected from different geographical regions and different plant hosts in Jordan. The results showed that, in addition to Bemisia tabaci, another nine different whitefly species were identified, including two species that were recorded for the first time in Jordan: Africaleurodes coffeacola, and Tetraleurodes neemani. A special focus has been given to economically important plant pests like the B. tabaci species complex. Three different diagnostic techniques were used to identify B. tabaci putative species based on mtCOI gene. All the collected samples of B. tabaci species complex were identified as Middle East–Asia Minor 1 (MEAM1) putative species.

Update on Cystic Fibrosis-Related Bone Disease: A Special Focus on Children

2009 ◽  
Vol 10 (3) ◽  
pp. 134-142 ◽  
Author(s):  
Isabelle Sermet-Gaudelus ◽  
Mireille Castanet ◽  
George Retsch-Bogart ◽  
Robert M. Aris
Keyword(s):  
Cystic Fibrosis ◽  
Bone Disease ◽  
Special Focus

Combination antifungal therapy for Scedosporium species in cystic fibrosis

2020 ◽  
Vol 55 (8) ◽  
pp. 1993-1995
Author(s):  
Siân Bentley ◽  
Jane C. Davies ◽  
Siobhán B. Carr ◽  
Ian M. Balfour‐Lynn
Keyword(s):  
Cystic Fibrosis ◽  
Antifungal Therapy ◽  
Scedosporium Species

Phylogeny of Hymenophyllum subg. Mecodium (Hymenophyllaceae), with special focus on the diversity of the Hymenophyllum polyanthos species complex

2019 ◽  
Vol 305 (9) ◽  
pp. 811-825 ◽  
Author(s):  
Diego T. Vasques ◽  
Atsushi Ebihara ◽  
Regina Y. Hirai ◽  
Jefferson Prado ◽  
Ito Motomi
Keyword(s):  
Species Complex ◽  
Special Focus
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