Involvement of selected cellular miRNAs in the in vitro and in vivo infection of infectious salmon anemia virus (ISAV)

2018 ◽  
Vol 123 ◽  
pp. 353-360 ◽  
Author(s):  
Carolina Salazar ◽  
Sergio H. Marshall
Keyword(s):  
Infectious Salmon Anemia Virus ◽  
Cellular Mirnas ◽  
Infectious Salmon Anemia ◽  
Salmon Anemia Virus ◽  
Anemia Virus

scholarly journals Chemical Synthesis andIn VitroEvaluation of a Phage Display-Derived Peptide Active against Infectious Salmon Anemia Virus

2016 ◽  
Vol 82 (8) ◽  
pp. 2563-2571 ◽  
Author(s):  
Nicolás Ojeda ◽  
Constanza Cárdenas ◽  
Fanny Guzmán ◽  
Sergio H. Marshall
Keyword(s):  
Phage Display ◽  
Specific Activity ◽  
Control Measures ◽  
Infectious Salmon Anemia Virus ◽  
Virus Binding ◽  
Fish Pathogens ◽  
Infectious Salmon Anemia ◽  
Salmon Anemia Virus ◽  
Anemia Virus

ABSTRACTInfectious salmon anemia virus (ISAV) is the etiological agent of the disease by the same name and causes major losses in the salmon industry worldwide. Epizootic ISAV outbreaks have occurred in Norway and, to a lesser degree, in Canada. In 2007, an ISAV outbreak in Chile destroyed most of the seasonal production and endangered the entire Chilean salmon industry. None of the existing prophylactic approaches have demonstrated efficacy in providing absolute protection from or even a palliative effect on ISAV proliferation. Sanitary control measures for ISAV, based on molecular epidemiology data, have proven insufficient, mainly due to high salmon culture densities and a constant presence of a nonpathogenic strain of the virus. This report describes an alternative treatment approach based on interfering peptides selected from a phage display library. The screening of a phage display heptapeptide library resulted in the selection of a novel peptide with significantin vitroantiviral activity against ISAV. This peptide specifically interacted with the viral hemagglutinin-esterase protein, thereby impairing virus binding, with plaque reduction assays showing a significant reduction in viral yields. The identified peptide acts at micromolar concentrations against at least two different pathogenic strains of the virus, without detectable cytotoxic effects on the tested fish cells. Therefore, antiviral peptides represent a novel alternative for controlling ISAV and, potentially, other fish pathogens.IMPORTANCEIdentifying novel methods for the efficient control of infectious diseases is imperative for the future of global aquaculture. The present study used a phage display heptapeptide library to identify a peptide with interfering activity against a key protein of the infectious salmon anemia virus (ISAV). A piscine orthomyxovirus, ISAV is a continuous threat to the commercial sustainability of cultured salmon production worldwide. The complex epidemiological strategy of this pathogen has made prophylactic control extremely difficult. The identified antiviral peptide efficiently impairs ISAV infectionin vitroby specifically blocking hemagglutinin-esterase, a pivotal surface protein of this virus. Peptide synthesis could further modify the primary structure of the identified peptide to improve specific activity and stability. The present results form the foundation for developing a new pharmacological treatment against ISAV.

Formation of autophagosomes and redistribution of LC3 upon in vitro infection with infectious salmon anemia virus

2010 ◽  
Vol 151 (1) ◽  
pp. 104-107 ◽  
Author(s):  
Berit Lyng Schiøtz ◽  
Norbert Roos ◽  
Anne-Lise Rishovd ◽  
Tor Gjøen
Keyword(s):  
Infectious Salmon Anemia Virus ◽  
Infectious Salmon Anemia ◽  
Salmon Anemia Virus ◽  
Anemia Virus

scholarly journals Dead Tumor Cells Expressing Infectious Salmon Anemia Virus Fusogenic Protein Favor Antigen Cross-Priming In Vitro

2017 ◽  
Vol 8 ◽  
Author(s):  
Jonathan Morales ◽  
Carlos Barrera-Avalos ◽  
Carlos Castro ◽  
Stephanie Castillo ◽  
Claudio Barrientos ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Infectious Salmon Anemia Virus ◽  
Infectious Salmon Anemia ◽  
Salmon Anemia Virus ◽  
Anemia Virus

scholarly journals Mechanism of cell death during infectious salmon anemia virus infection is cell type-specific

2004 ◽  
Vol 85 (10) ◽  
pp. 3027-3036 ◽  
Author(s):  
Tomy Joseph ◽  
Arnost Cepica ◽  
Laura Brown ◽  
Basil O. Ikede ◽  
Frederick S. B. Kibenge
Keyword(s):  
Cell Death ◽  
Fish Cell ◽  
Infectious Salmon Anemia Virus ◽  
Cell Type ◽  
Hmgb1 Protein ◽  
Infectious Salmon Anemia ◽  
Salmon Anemia Virus ◽  
Induced Apoptosis ◽  
Anemia Virus

Infectious salmon anemia virus (ISAV) is a very important fish virus in the Northern hemisphere and there is continued interest in understanding the mechanisms of its pathogenesis and persistence in fish. In this study, the permissive fish cell lines SHK-1, CHSE-214 and TO were used to determine whether ISAV-induced cytopathic effect (CPE) is due to apoptosis or necrosis. Characteristic apoptotic DNA fragmentation was observed only in ISAV-infected SHK-1 and CHSE-214 cells. Apoptosis in ISAV-infected SHK-1 cells was confirmed by fragment end-labelling assay, suggesting that CPE in these cells is associated with apoptosis. ISAV-infected TO cells did not undergo apoptosis, but showed leakage of high-mobility group 1 (HMGB1) protein from the nucleus, which is characteristic of cells undergoing necrosis; this suggests that CPE in these cells is associated with necrosis. ISAV-infected SHK-1 cells did not show leakage of HMGB1 protein. Infection with two different strains of ISAV showed that induction of apoptosis was correlated with the appearance of CPE in SHK-1 cells. ISAV-induced apoptosis was inhibited by a pan-caspase inhibitor, Z-VAD-fmk, indicating a caspase-activation pathway. The ISAV putative PB2 protein and proteins encoded by RNA segment 7 bound caspase-8 specifically in vitro, suggesting that these viral proteins may have a role in ISAV-induced apoptosis. These findings demonstrate for the first time that the mechanism of cell death during ISAV infection is dependent on the cell type, which may have implications for ISAV pathogenesis and persistence.

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