Molecular characterization of Clostridium difficile isolated from carriage and association of its pathogenicity to prevalent toxic genes

2018 ◽  
Vol 120 ◽  
pp. 1-7
Author(s):  
Abdulwahab Ali Abuderman ◽  
Ayesha Mateen ◽  
Rabbani Syed ◽  
Mustafa sawsan aloahd
Keyword(s):  
Clostridium Difficile ◽  
Molecular Characterization

Molecular characterization of moxifloxacin resistance from Canadian Clostridium difficile clinical isolates

2010 ◽  
Vol 66 (4) ◽  
pp. 419-424 ◽  
Author(s):  
Andrew Walkty ◽  
David A. Boyd ◽  
Denise Gravel ◽  
Jim Hutchinson ◽  
Allison McGeer ◽  
...  
Keyword(s):  
Clostridium Difficile ◽  
Molecular Characterization ◽  
Clinical Isolates

scholarly journals Molecular Characterization of Clostridium difficile Isolates in China From 2010 to 2015

2018 ◽  
Vol 9 ◽  
Author(s):  
Xiao-shu Liu ◽  
Wen-ge Li ◽  
Wen-zhu Zhang ◽  
Yuan Wu ◽  
Jin-xing Lu
Keyword(s):  
Clostridium Difficile ◽  
Molecular Characterization

scholarly journals Molecular Characterization and Moxifloxacin Susceptibility of Clostridium difficile

Antibiotics ◽  
2019 ◽  
Vol 8 (3) ◽  
pp. 118
Author(s):  
Mizrahi ◽  
Hamo ◽  
Azrad ◽  
Peretz
Keyword(s):  
Clostridium Difficile ◽  
Molecular Characterization ◽  
Dna Extraction ◽  
Resistance Testing ◽  
Gyra Gene ◽  
Low Frequencies ◽  
High Concordance ◽  
Stool Samples ◽  
Different Strains

In recent years, the incidence and severity of Clostridium difficile infections has increased.Additionally, resistance of C. difficile to frequently used antibiotics is rising. To improve ourunderstanding of C. difficile, there is a need for molecular characterization of different strains andantibiotic resistance testing. We investigated the efficacy of GenoType CDiff kit (Hain Lifesciences)in identification of C. difficile and its various strains in northern Israel. The kit involves a molecularassay that detects C. difficile from stool samples or colonies and identifies the different strains andmutations in the gyrA gene that cause moxifloxacin resistance. Forty‐nine C. difficile positive sampleswere examined by the kit following DNA extraction from both colonies and stool. The identificationrate (95.9%) of C. difficile was much higher when DNA was extracted from colonies, compared toextraction from stool (46.9%). Low frequencies of ribotype027 strain (2%) and of ribotype078 strain(4%) were found. There was a high concordance between genotype (mutation in gyrA) andphenotype (Etest) for moxifloxacin resistance (Kappa=0.72). A high percentage of moxifloxacinresistantstrains was found. Our findings indicate that the GenoType CDiff kit is very effective incharacterization of C. difficile strains and less effective for identification of C. difficile directly fromstool samples.

scholarly journals Prevalence and molecular characterization of Clostridium difficile isolated from feedlot beef cattle upon arrival and mid-feeding period

2012 ◽  
Vol 8 (1) ◽  
pp. 38 ◽  
Author(s):  
Marcio C Costa ◽  
Richard Reid-Smith ◽  
Sheryl Gow ◽  
Sherry J Hannon ◽  
Calvin Booker ◽  
...  
Keyword(s):  
Clostridium Difficile ◽  
Beef Cattle ◽  
Molecular Characterization ◽  
Feeding Period

scholarly journals Molecular Characterization of fliD Gene Encoding Flagellar Cap and Its Expression among Clostridium difficile Isolates from Different Serogroups

2001 ◽  
Vol 39 (3) ◽  
pp. 1178-1183 ◽  
Author(s):  
A. Tasteyre ◽  
T. Karjalainen ◽  
V. Avesani ◽  
M. Delmee ◽  
A. Collignon ◽  
...  
Keyword(s):  
Clostridium Difficile ◽  
Molecular Characterization ◽  
Gene Encoding
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