Synthesis of silver nanoparticles from Bacillus brevis (NCIM 2533) and their antibacterial activity against pathogenic bacteria

2018 ◽  
Vol 116 ◽  
pp. 221-226 ◽  
Author(s):  
Muthupandian Saravanan ◽  
Sisir Kumar Barik ◽  
Davoodbasha MubarakAli ◽  
Periyakaruppan Prakash ◽  
Arivalagan Pugazhendhi
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Pathogenic Bacteria ◽  
Bacillus Brevis

scholarly journals Fabrication of Antimicrobial Perspiration Pads and Cotton Cloth UsingAmaranthus dubiusMediated Silver Nanoparticles

2013 ◽  
Vol 2013 ◽  
pp. 1-5 ◽  
Author(s):  
M. Jannathul Firdhouse ◽  
P. Lalitha
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Medical Devices ◽  
Food Industry ◽  
Pathogenic Bacteria ◽  
Antibacterial Agents ◽  
Cotton Cloth ◽  
Textile Materials ◽  
Textile Fabrics ◽  
Antibacterial Textile

Silver nanoparticles prepared through a simplistic method using the aqueous extract ofAmaranthus dubiuswere fabricated on perspiration pads and cotton cloth samples to obtain antibacterial textile materials by two different fabrication methods. The antibacterial activity was investigated against the bacteriaCorynebacteriumwhich is commonly present in sweat. Silver nanoparticles that serve as antibacterial agents, against pathogenic bacteria, have gained increased applications in medical devices, textile fabrics, and food industry and hence the result of this study would be a welcomed option.

scholarly journals Biomimetic Synthesis of Silver Nanoparticles from Aqueous Extract of Saraca indica and its Profound Antibacterial Activity

2020 ◽  
Vol 11 (1) ◽  
pp. 8110-8120
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Aqueous Extract ◽  
Silver Nanoparticle ◽  
Pathogenic Bacteria ◽  
Drug Formulation ◽  
Biomimetic Synthesis ◽  
Visible Absorption ◽  
The Stability ◽  
Average Size

The present findings were focused on green synthesis of silver nanoparticles through an aqueous extract of Saraca indica. The stability of the nanoparticle was achieved through the optimization of physico-chemical parameters. The sharp UV-visible absorption maximum at 400 was observed for biological synthesized silver nanoparticles. The spectroscopic analysis was thus used to assess the formation of silver nanoparticles. The AFM analysis did analyze the morphology of the nanocomposite, which was further confirmed through TEM micrograph. The electron micrograph image discloses that silver nanoparticles were polydispersed and dominantly as spherical with size ranges from 40nm to 100nm. The average size distribution was 49nm. The chemical reductions of Ag+ ions were further confirmed through FTIR. The biogenic silver nanoparticle and their drug formulation showed profound antibacterial activity against pathogenic bacteria. The flavonoids rich binding of silver nanoparticle showed great medicinal potential and can be used for the treatment of several harmful infectious diseases. Hence, plant-based metal nanoparticles meet the demand for less toxic formulation during drug development and its delivery.

scholarly journals Green Synthesis Concept of Nanoparticles From Environmental Bacteria and Their Effects on Pathogenic Bacteria

2020 ◽  
pp. 1289-1297
Author(s):  
Ghada Mohammed Saleh
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Soil Bacteria ◽  
Pathogenic Bacteria ◽  
Pseudomonas Sp ◽  
Good Source ◽  
Force Microscopy ◽  
Atomic Force ◽  
Environmental Bacteria ◽  
Inhibition Zones

Soil bacteria play an interesting role in the reduction of Ag+ ions and the formation of silver nanoparticles (AgNPs), which may be a good source for nanoparticles and play a major role in nanotechnology applications. The concept of this project was to study the effects of these environmentally produced nanoparticles on the growth of some pathogenic bacteria. The environmental bacteria were isolated from soil, purified on broth cultures, and centrifuged, while the supernatant was extracted to detect its ability to convert silver nitrate to nanoparticles. The AgNPs was detected by Atomic Force Microscopy (AFM), while Granularity Cumulating Distribution (GCD) was employed to estimate the AgNPs sizes. The results showed the synthesis of AgNPs with sizes of 63.50nm and 45.81nm from the extracts of environmental Pseudomonas sp. and Enterobacter, respectively. The synthesized AgNPs from the extracts of all environmental bacteria showed antibacterial activity against some pathogenic bacteria (Gram positive and Negative) with variable inhibition zones. In conclusion, environmental bacteria can be a cheap source of nanoparticles.

scholarly journals ISOLASI, KARAKTERISASI DAN IDENTIFIKASI BAKTERI ENDOFIT KULIT BATANG SRIKAYA (ANNONA SQUAMOSA) DAN POTENSINYA SEBAGAI ANTIBAKTERI

2018 ◽  
Vol 4 (1) ◽  
Author(s):  
Lalu Zulkifli ◽  
Dwi Soelistya Dyah Jekti ◽  
Samsul Bahri
Keyword(s):  
Antibacterial Activity ◽  
Endophytic Bacteria ◽  
Pathogenic Bacteria ◽  
Positive Control ◽  
Spore Formation ◽  
Bacterial Isolates ◽  
Annona Squamosa ◽  
Biochemical Tests ◽  
Bacillus Brevis ◽  
E Coli

The objective of this study was to isolate endophytic bacteria from bark of srikaya, analyzing antibacterial activity of endophytic bacteria in S. aureus, B. cereus and E. coli pathogenic bacteria, characterizing endophytic bacteria capable of inhibiting the growth of pathogenic bacteria and identification of endophytic bacteria able to inhibit the growth of pathogenic bacteria. Isolation of endophytic bacteria using TSA and NA media, bioassay on pathogen bacteria with concentration of 106 cells / ml with using Ø 6 mm wells and entering supernatant of 100 μl. Supernatant was obtained by growing endophytic bacteria in NB media shaken with shaker 150 cycles / min for 48 h at 32ᵒC then culture centrifuge at 5000 g for 30 min. Positive control using cyprofloxacin. Characterization is based on the nature of the colony, Gram paint, spore formation, and biochemical tests. The results of the study yielded 13 endophytic bacterial isolates and 4 endophytic isolates capable of inhibiting the growth of 8 pathogenic bacteria with sensitive criteria, 2 pathogenic bacteria with resistant criteria and 1 pathogen bacteria can not be inhibited its growth. Gram's paint results show that 4 endophytic isolates belong to Gram-positive, rod-shaped and spore forming cells. From the character possessed by the bacteria can be identified that the 4 bacteria endofit capable of inhibiting pathogenic bacteria are Bacillus brevis, Bacillus latesporus, Virgibacillus pantothenticus, and Bacillus circulansKeywords: Characterization, Endophytic Bacteria, Bark of Srikaya, Antibacterial

scholarly journals Green synthesis of bio-molecule encapsulated magnetic silver nanoparticles and their antibacterial activity

RSC Advances ◽  
2018 ◽  
Vol 8 (65) ◽  
pp. 37176-37183 ◽  
Author(s):  
A. K. M. Atique Ullah ◽  
M. F. Kabir ◽  
M. Akter ◽  
A. N. Tamanna ◽  
A. Hossain ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Green Synthesis ◽  
Pathogenic Bacteria ◽  
Ag Nps ◽  
Synthesis Technique

Persuaded by the necessity of finding new sources of antibiotics, Ag NPs were synthesized by adopting a newly developed green synthesis technique and subsequently, their antibacterial activity against different pathogenic bacteria was evaluated.

Biological Applications, Green Synthesis, Characterization of Silver Nanoparticles (AgNPs) Using Artemisia annua L.

2021 ◽  
Vol 13 (7) ◽  
pp. 1304-1309
Author(s):  
Hamed A. Ghramh ◽  
Rahmah N. Al-Qthanin ◽  
Zubair Ahmad ◽  
Essam H. Ibrahim ◽  
Mona Kilany ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Pathogenic Bacteria ◽  
Artemisia Annua ◽  
Diffusion Method ◽  
Biological Applications ◽  
Disk Diffusion Method ◽  
Vis Spectroscopy ◽  
Electron Microscopy Analysis ◽  
Microscopy Analysis

ABSTRACTThis article reports on the silver nanoparticles (AaAgNPs) that were green-synthesized by using Artemisia annua L. extract (AaExt) and their collective biological applications. Active biomolecules in the extract and extract containing AgNPs were characterized using Fourier-transform-infrared-spectroscopy (FTIR) and AgNPs were monitored by UV/vis spectroscopy and SEM (scanning electron microscopy) analysis. The size of the particle is around 100 nm. The antibacterial activity was measured by the disk diffusion method against the Gram-negative/positive pathogenic bacteria. The extract and extract containing AgNPs showed a significant antibacterial activity. Cytotoxic potential of the synthesized AgNPs was analyzed against the rat splenocytes. The results showed that there were cytotoxic effects of A. annua leaves extract but stimulatory effects when the extract contained AgNPs on normal splenocytes. Extract of A. annua showed very little increase in liver enzymes. Regarding the larvicidal activity, the extract containing AgNPs was more effective than the crude leaves extract against 4th instar larvae of Culex pipiens (LC50 = 171.378 ppm) compared to the plant extract (LC50 = 5389.726 ppm) by about 31.449 folds.

scholarly journals Enhancement of the antibacterial potential of plantaricin by incorporation into silver nanoparticles

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Sara Adel Amer ◽  
Hala Mohamed Abushady ◽  
Rasha Mohamed Refay ◽  
Mahmoud Ahmed Mailam
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Foodborne Pathogens ◽  
Inhibitory Activity ◽  
Pathogenic Bacteria ◽  
Antibacterial Activities ◽  
Stability Period ◽  
Genes Encoding ◽  
Pharmaceutical Industries ◽  
Antibacterial Potential

Abstract Background Bacteriocins are proteinaceous compounds produced from lactic acid bacteria. Bacteriocins are well-known for their antibacterial potential and safety for application in food. However, the commercial availability of bacteriocin is facing several limitations; among them is the low yield and short stability period. That calls for a new strategy for overcoming these hurdles. Among these approaches is incorporating bacteriocin in nanoparticles. So, the aim of this study was to enhance the plantaricin produced from isolated Lactobacillus plantarum strain using nanotechnology. Results In this study, the plnEF genes encoding plantaricin EF have been identified and sequenced (accession number of MN172264.1). The extracted bacteriocin (EX-PL) was obtained by the ammonium sulfate method. Then, it was used for biosynthesizing plantaricin-incorporated silver nanoparticles (PL-SNPs). The synthesized nanoparticles were confirmed by SEM-EDAX analysis. The antibacterial activity of both combined (PL-SNPs) and extracted plantaricin (EX-PL) were tested against some strains of foodborne pathogenic bacteria. The results revealed that the antibacterial activities were increased by 99.2% on the combination of bacteriocin with the silver nanoparticle. The MIC of EX-PL (7.6 mg/mL) has been lowered after incorporating into silver nanoparticles and reached 0.004 mg/mL for PL-SNPs. Despite that extracted plantaricin showed no inhibitory activity towards Listeria monocytogenes, plantaricin-incorporated silver nanoparticles displayed inhibitory activity against this strain. Furthermore, the stability period at 4 °C was increased from 5 days to 60 days for EX-PL and PL-SNPs, respectively. Conclusions Plantaricin-incorporated silver nanoparticles possess higher antibacterial activity and more stability than the free one, which makes it more fitting for combating foodborne pathogens and open more fields for applications in both food and pharmaceutical industries. Graphical abstract

scholarly journals Antibacterial Activity of Silver Nanoparticles Synthesized from Plant Latex

2020 ◽  
pp. 1579-1588
Author(s):  
Ghada Mohammed Saleh ◽  
Shaymaa Suhail Najim
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Pathogenic Bacteria ◽  
Medical Field ◽  
Gram Negative ◽  
Force Microscopy ◽  
Industrial Project ◽  
Atomic Force ◽  
Diffusion Assay ◽  
Plant Latex

     Nanoparticles produced by plants are preferred in the medical field for its safe and unpolluted product; it is also accepted as an ecofriendly, non-expensive, and non-toxic nanomaterial. In this study, silver nitrate was successfully used to produce silver nanoparticles (AgNPs) by the use extractsof 4 different latex-producing plants which belong to 2 families (Moraceae and Euphorbiaceae). The synthesis was proved by Atomic Force Microscopy (AFM).The sizes of the AgNP grains were estimated by Granularity Cumulating Distribution (GCD). The results revealed the production of AgNPs in different sizes of 103 and 82 nm using the Moraceae family and 77 and74nm using the Euphorbiaceae family.Antibacterial activity was also detected against both Gram positive and Gram negative pathogenic bacteria using the well diffusion assay. In conclusion, this source of nanoparticles can be a very useful industrial project in a goal to find new safe and economic alternatives to antibiotics.

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