Biosynthesis, characterization and antimicrobial activities of zinc oxide nanoparticles from leaf extract of Glycosmis pentaphylla (Retz.) DC

2018 ◽  
Vol 116 ◽  
pp. 44-48 ◽  
Author(s):  
S. Vijayakumar ◽  
C. Krishnakumar ◽  
P. Arulmozhi ◽  
S. Mahadevan ◽  
N. Parameswari
Keyword(s):  
Zinc Oxide ◽  
Zinc Oxide Nanoparticles ◽  
Leaf Extract ◽  
Antimicrobial Activities ◽  
Oxide Nanoparticles

Natural fuels (Honey and Cow urine) assisted combustion synthesis of zinc oxide nanoparticles for antimicrobial activities

2021 ◽  
Vol 47 (10) ◽  
pp. 14475-14481
Author(s):  
B. Ranjithkumar ◽  
H.B. Ramalingam ◽  
E. Ranjith Kumar ◽  
Ch. Srinivas ◽  
G. Magesh ◽  
...  
Keyword(s):  
Zinc Oxide ◽  
Combustion Synthesis ◽  
Zinc Oxide Nanoparticles ◽  
Antimicrobial Activities ◽  
Oxide Nanoparticles ◽  
Cow Urine

Genotoxic assay of silver and zinc oxide nanoparticles synthesized by leaf extract of Garcinia livingstonei T. Anderson: A comparative study

2021 ◽  
Vol 17 (5) ◽  
pp. 114
Author(s):  
ShivaPrasad Kollur ◽  
AzharuddinB Daphedar ◽  
SiddappaB Kakkalameli ◽  
Govindappa Melappa ◽  
TarikereChandrashekharappa Taranath ◽  
...  
Keyword(s):  
Zinc Oxide ◽  
Comparative Study ◽  
Zinc Oxide Nanoparticles ◽  
Leaf Extract ◽  
Oxide Nanoparticles

scholarly journals Crotalaria verrucosa Leaf Extract Mediated Synthesis of Zinc Oxide Nanoparticles: Assessment of Antimicrobial and Anticancer Activity

Molecules ◽  
2020 ◽  
Vol 25 (21) ◽  
pp. 4896
Author(s):  
Siva Sankar Sana ◽  
Divya Vishambhar Kumbhakar ◽  
Akbar Pasha ◽  
Smita C. Pawar ◽  
Andrews Nirmala Grace ◽  
...  
Keyword(s):  
Zinc Oxide ◽  
Cell Migration ◽  
Cell Lines ◽  
Zinc Oxide Nanoparticles ◽  
Leaf Extract ◽  
Positive Control ◽  
Ftir Spectra ◽  
Total Phenolic ◽  
Oxide Nanoparticles ◽  
Zno Nps

In this work, we present an ecofriendly, non-hazardous, green synthesis of zinc oxide nanoparticles (ZnO NPs) by leaf extract of Crotalaria verrucosa (C. verrucosa). Total phenolic content, total flavonoid and total protein contents of C. verrucosa were determined. Further, synthesized ZnO NPs was characterized by UV–visible spectroscopy (UV-vis), X-ray diffractometer (XRD), Fourier transform infra-red (FTIR) Spectra, transmission electron microscope (TEM), and Dynamic light scattering (DLS) analysis. UV-vis shows peak at 375 nm which is unique to ZnO NPs. XRD analysis demonstrates the hexagonal phase structures of ZnO NPs. FTIR spectra demonstrates the molecules and bondings associated with the synthesized ZnO NPs and assures the role of phytochemical compounds of C. verrucosa in reduction and capping of ZnO NPs. TEM image exhibits that the prepared ZnO NPs is hexagonal shaped and in size ranged between 16 to 38 nm which is confirmed by DLS. Thermo-gravimetric analysis (TGA) was performed to determine the thermal stability of biosynthesized nanoparticles during calcination. The prepared ZnO NPs showed significant antibacterial potentiality against Gram-positive (S. aureus) and Gram-negative (Proteus vulgaris, Klebsiella pneumoniae, and Escherichia coli) pathogenic bacteria and SEM image shows the generalized mechanism of action in bacterial cell after NPs internalization. In addition, NPs are also found to be effective against the studied cancer cell lines for which cytotoxicity was assessed using MTT assay and results demonstrate highest growth of inhibition at the concentration of 100 µg/mL with IC50 value at 7.07 µg/mL for HeLa and 6.30 µg/mL for DU145 cell lines, in contrast to positive control (C. verrucosa leaf extract) with IC50 of 22.30 µg/mL on HeLa cells and 15.72 µg/mL on DU145 cells. Also, DAPI staining was performed in order to determine the effect on nuclear material due to ZnO NPs treatment in the studied cell lines taking leaf extract as positive control and untreated negative control for comparison. Cell migration assay was evaluated to determine the direct influence of NPs on metastasis that is potential suppression capacity of NPs to tumor cell migration. Outcome of the synthesized ZnO NPs using C. verrucosa shows antimicrobial activity against studied microbes, also cytotoxicity, apoptotic mediated DNA damage and antiproliferative potentiality in the studied carcinoma cells and hence, can be further used in biomedical, pharmaceutical and food processing industries as an effective antimicrobial and anti-cancerous agent.

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