Helicobacter pylori infection increases sirt2 gene expression in gastric epithelial cells of gastritis patients

2018 ◽  
Vol 116 ◽  
pp. 120-123 ◽  
Author(s):  
Seiran Zandi ◽  
Manouchehr A. Hedayati ◽  
Ebrahim Mohammadi ◽  
Farshad Sheikhesmaeili
Keyword(s):  
Gene Expression ◽  
Helicobacter Pylori ◽  
Epithelial Cells ◽  
Helicobacter Pylori Infection ◽  
Gastric Epithelial Cells ◽  
Sirt2 Gene

Lactobacillus fermentum UCO-979C beneficially modulates the innate immune response triggered by Helicobacter pylori infection in vitro

2018 ◽  
Vol 9 (5) ◽  
pp. 829-841 ◽  
Author(s):  
V. Garcia-Castillo ◽  
H. Zelaya ◽  
A. Ilabaca ◽  
M. Espinoza-Monje ◽  
R. Komatsu ◽  
...  
Keyword(s):  
Immune Response ◽  
Helicobacter Pylori ◽  
Epithelial Cells ◽  
Morphological Changes ◽  
Helicobacter Pylori Infection ◽  
Lactobacillus Fermentum ◽  
Gastric Tissue ◽  
Gastric Epithelial Cells ◽  
Ags Cells ◽  
H Pylori

Helicobacter pylori infection is associated with important gastric pathologies. An aggressive proinflammatory immune response is generated in the gastric tissue infected with H. pylori, resulting in gastritis and a series of morphological changes that increase the susceptibility to cancer development. Probiotics could present an alternative solution to prevent or decrease H. pylori infection. Among them, the use of immunomodulatory lactic acid bacteria represents a promising option to reduce the severity of chronic inflammatory-mediated tissue damage and to improve protective immunity against H. pylori. We previously isolated Lactobacillus fermentum UCO-979C from human gastric tissue and demonstrated its capacity to reduce adhesion of H. pylori to human gastric epithelial cells (AGS cells). In this work, the ability of L. fermentum UCO-979C to modulate immune response in AGS cells and PMA phorbol 12-myristate 13-acetate (PMA)-differentiated THP-1 (human monocytic leukaemia) macrophages in response to H. pylori infection was evaluated. We demonstrated that the UCO-979C strain is able to differentially modulate the cytokine response of gastric epithelial cells and macrophages after H. pylori infection. Of note, L. fermentum UCO-979C was able to significantly reduce the production of inflammatory cytokines and chemokines in AGS and THP-1 cells as well as increase the levels of immunoregulatory cytokines, indicating a remarkable anti-inflammatory effect. These findings strongly support the probiotic potential of L. fermentum UCO-979C and provide evidence of its beneficial effects against the inflammatory damage induced by H. pylori infection. Although our findings should be proven in appropriate experiments in vivo, in both H. pylori infection animal models and human trials, the results of the present work provide a scientific rationale for the use of L. fermentum UCO-979C to prevent or reduce H. pylori-induced gastric inflammation in humans.

scholarly journals Inhibitory Effect of β-Carotene on Helicobacter pylori-Induced TRAF Expression and Hyper-Proliferation in Gastric Epithelial Cells

Antioxidants ◽  
2019 ◽  
Vol 8 (12) ◽  
pp. 637 ◽  
Author(s):  
Yongchae Park ◽  
Hanbit Lee ◽  
Joo Weon Lim ◽  
Hyeyoung Kim
Keyword(s):  
Gene Expression ◽  
Gastric Cancer ◽  
Helicobacter Pylori ◽  
Epithelial Cells ◽  
Nadph Oxidase ◽  
Gastric Epithelial Cells ◽  
Gastric Cancer Cells ◽  
Ags Cells ◽  
H Pylori ◽  
Β Carotene

Helicobacter pylori infection causes the hyper-proliferation of gastric epithelial cells that leads to the development of gastric cancer. Overexpression of tumor necrosis factor receptor associated factor (TRAF) is shown in gastric cancer cells. The dietary antioxidant β-carotene has been shown to counter hyper-proliferation in H. pylori-infected gastric epithelial cells. The present study was carried out to examine the β-carotene mechanism of action. We first showed that H. pylori infection decreases cellular IκBα levels while increasing cell viability, NADPH oxidase activity, reactive oxygen species production, nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) activation, and TRAF1 and TRAF2 gene expression, as well as protein–protein interaction in gastric epithelial AGS cells. We then demonstrated that pretreatment of cells with β-carotene significantly attenuates these effects. Our findings support the proposal that β-carotene has anti-cancer activity by reducing NADPH oxidase-mediated production of ROS, NF-κB activation and NF-κB-regulated TRAF1 and TRAF2 gene expression, and hyper-proliferation in AGS cells. We suggest that the consumption of β-carotene-enriched foods could decrease the incidence of H. pylori-associated gastric disorders.

scholarly journals Helicobacter pylori-Induced Histone Modification, Associated Gene Expression in Gastric Epithelial Cells, and Its Implication in Pathogenesis

PLoS ONE ◽  
2010 ◽  
Vol 5 (4) ◽  
pp. e9875 ◽  
Author(s):  
Song-Ze Ding ◽  
Wolfgang Fischer ◽  
Maria Kaparakis-Liaskos ◽  
George Liechti ◽  
D. Scott Merrell ◽  
...  
Keyword(s):  
Gene Expression ◽  
Helicobacter Pylori ◽  
Epithelial Cells ◽  
Histone Modification ◽  
Gastric Epithelial Cells

The effects of Helicobacter pylori infection on the signal transduction of gastric epithelial cells

2003 ◽  
Vol 124 (4) ◽  
pp. A589
Author(s):  
Takeshi Azuma ◽  
Shiho Yamazaki ◽  
Akiyo Yamakawa ◽  
Masahiro Ohtani ◽  
Yoshiyuki Ito ◽  
...  
Keyword(s):  
Signal Transduction ◽  
Helicobacter Pylori ◽  
Epithelial Cells ◽  
Helicobacter Pylori Infection ◽  
Gastric Epithelial Cells

Nod1-dependent proinflammatory responses to Helicobacter pylori infection in gastric epithelial cells

2003 ◽  
Vol 124 (4) ◽  
pp. A43 ◽  
Author(s):  
Jerome Viala ◽  
Catherine Chaput ◽  
Ivo G. Boneca ◽  
Stephen E. Girardin ◽  
Anthony P. Moran ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Epithelial Cells ◽  
Helicobacter Pylori Infection ◽  
Gastric Epithelial Cells ◽  
Proinflammatory Responses

The Effect of Helicobacter pylori Infection on Levels of DNA Damage in Gastric Epithelial Cells

Helicobacter ◽  
2002 ◽  
Vol 7 (5) ◽  
pp. 271-280 ◽  
Author(s):  
S. M. Everett ◽  
K. L. M. White ◽  
I. M. Drake ◽  
C. J. Schorah ◽  
R. J. Calvert ◽  
...  
Keyword(s):  
Dna Damage ◽  
Helicobacter Pylori ◽  
Epithelial Cells ◽  
Helicobacter Pylori Infection ◽  
Gastric Epithelial Cells
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