Genetic diversity, phylogroup distribution and virulence gene profile of pks positive Escherichia coli colonizing human intestinal polyps

2017 ◽  
Vol 112 ◽  
pp. 274-278 ◽  
Author(s):  
Meysam Sarshar ◽  
Daniela Scribano ◽  
Massimiliano Marazzato ◽  
Cecilia Ambrosi ◽  
Maria Rita Aprea ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Genetic Diversity ◽  
Virulence Gene ◽  
Gene Profile ◽  
Intestinal Polyps ◽  
Virulence Gene Profile

Serological diversity, molecular characterisation and antimicrobial sensitivity of avian pathogenic Escherichia coli (APEC) isolates from broiler chickens in Kashmir, India

2019 ◽  
Vol 59 (2) ◽  
pp. 338
Author(s):  
S. N. Magray ◽  
S. A. Wani ◽  
Z. A. Kashoo ◽  
M. A. Bhat ◽  
S. Adil ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Broiler Chickens ◽  
Virulence Genes ◽  
Virulence Gene ◽  
Gene Profile ◽  
Avian Pathogenic Escherichia Coli ◽  
Pathogenic Escherichia Coli ◽  
Significant Difference ◽  
Virulence Gene Profile

The present study has determined the serological diversity, virulence-gene profile and in vitro antibiogram of avian pathogenic Escherichia coli (APEC) isolates from broiler chickens in India suspected to have died of colibacillosis. The virulence-gene profile of APEC was compared with that of the Escherichia coli isolates from faeces of apparently healthy chickens, called avian faecal E. coli (AFEC). In total, 90 representative isolates of APEC and 63 isolates of AFEC were investigated in the present study. The APEC were typed into 19 serogroups, while some isolates were rough and could not be typed. Most prevalent serogroup was O2 (24.44%). Among the eight virulence genes studied, the prevalence of seven genes (iss, iucD, tsh, cva/cvi, irp2, papC and vat) was significantly higher in APEC than in AFEC isolates. However, there was no significant difference between APEC and AFEC isolates for possession of astA gene. The most frequent gene detected among the two groups of organisms was iss, which was present in 98.88% and 44.44% of APEC and AFEC isolates respectively. The in vitro antibiogram showed that the majority (96.6%) of APEC isolates were resistant to tetracycline, while 82.2% were resistant to cephalexin, 78.8% to cotrimoxazole, 68.8% to streptomycin and 63.3% to ampicillin. However, most of them (84.45%) were sensitive to gentamicin. Thus, it is concluded that APEC from the broiler chickens carried putative virulence genes that attributed to their pathogenicity. Furthermore, the majority of APEC isolates were found to be multi-drug resistant, which, in addition to leading treatment failures in poultry, poses a public health threat.

scholarly journals In silico phylogenetic and virulence gene profile analyses of avian pathogenic Escherichia coli genome sequences

2014 ◽  
Vol 34 (2) ◽  
pp. 129-133 ◽  
Author(s):  
Thaís C.G. Rojas ◽  
Renato P. Maluta ◽  
Luciano V. Koenigkan ◽  
Wanderley Dias da Silveira
Keyword(s):  
Escherichia Coli ◽  
Phylogenetic Tree ◽  
In Silico ◽  
Virulence Gene ◽  
Gene Profile ◽  
Avian Pathogenic Escherichia Coli ◽  
Genome Sequences ◽  
E Coli ◽  
Pathogenic Escherichia Coli ◽  
Virulence Gene Profile

Avian pathogenic Escherichia coli (APEC) infections are responsible for significant losses in the poultry industry worldwide. A zoonotic risk has been attributed to APEC strains because they present similarities to extraintestinal pathogenic E. coli (ExPEC) associated with illness in humans, mainly urinary tract infections and neonatal meningitis. Here, we present in silico analyses with pathogenic E. coli genome sequences, including recently available APEC genomes. The phylogenetic tree, based on multi-locus sequence typing (MLST) of seven housekeeping genes, revealed high diversity in the allelic composition. Nevertheless, despite this diversity, the phylogenetic tree was able to cluster the different pathotypes together. An in silico virulence gene profile was also determined for each of these strains, through the presence or absence of 83 well-known virulence genes/traits described in pathogenic E. coli strains. The MLST phylogeny and the virulence gene profiles demonstrated a certain genetic similarity between Brazilian APEC strains, APEC isolated in the United States, UPEC (uropathogenic E. coli) and diarrheagenic strains isolated from humans. This correlation corroborates and reinforces the zoonotic potential hypothesis proposed to APEC.

scholarly journals A comparative survey between non-systemic Salmonella spp. (paratyphoid group) and systemic Salmonella Pullorum and S. Gallinarum with a focus on virulence genes

2017 ◽  
Vol 37 (10) ◽  
pp. 1064-1068 ◽  
Author(s):  
Claudete S. Astolfi-Ferreira ◽  
Marcelo R.S. Pequini ◽  
Luis F.N. Nuñez ◽  
Silvana H. Santander Parra ◽  
Ruy Chacon ◽  
...  
Keyword(s):  
Virulence Genes ◽  
Virulence Gene ◽  
Gene Profile ◽  
Salmonella Spp ◽  
Salmonella Pullorum ◽  
Comparative Survey ◽  
Virulence Gene Profile

ABSTRACT: A comparative survey between non-systemic (paratyphoid Salmonellae) and systemic (S. Pullorum and S. Gallinarum) Salmonella strains was performed to produce a virulence gene profile for differentiation among the groups. The following virulence genes were evaluated: invA, spvC, sefC, pefA, fimY, sopB, sopE1, stn and avrA. There are substantial differences among paratyphoid Salmonellae, S. Pullorum, and S. Gallinarum regarding the genes sefC, spvC, sopE1 and avrA. A higher frequency of sefC, spvC, sopE1 and avrA genes were detected in S. Gallinarum and S. Pullorum when compared with strains from the paratyphoid group of Salmonella. These results may be useful for differentiating among different groups and serotypes.

scholarly journals Prevalence of Campylobacter species in milk and milk products, their virulence gene profile and antibiogram

2015 ◽  
Vol 8 (1) ◽  
pp. 1-8 ◽  
Author(s):  
Shivani Modi ◽  
M. N. Brahmbhatt ◽  
Y. A. Chatur ◽  
J. B. Nayak
Keyword(s):  
Virulence Gene ◽  
Gene Profile ◽  
Milk Products ◽  
Campylobacter Species ◽  
Virulence Gene Profile
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