Decrease of colonization in the chicks' cecum and internal organs of Salmonella enterica serovar Pullorum by deletion of cpdB by Red system

2015 ◽  
Vol 80 ◽  
pp. 21-26 ◽  
Author(s):  
Huifang Liu ◽  
Liping Chen ◽  
Xiumei Wang ◽  
Wei Si ◽  
Huanan Wang ◽  
...  
Keyword(s):  
Salmonella Enterica ◽  
Internal Organs ◽  
Salmonella Enterica Serovar Pullorum

Persistence of Orally Administered Salmonella enterica Serovars Agona and Montevideo in Atlantic Salmon (Salmo salar L.)

2005 ◽  
Vol 68 (7) ◽  
pp. 1336-1339 ◽  
Author(s):  
L. L. NESSE ◽  
T. LØVOLD ◽  
B. BERGSJØ ◽  
K. NORDBY ◽  
C. WALLACE ◽  
...  
Keyword(s):  
Gastrointestinal Tract ◽  
Atlantic Salmon ◽  
Salmo Salar ◽  
Salmonella Enterica ◽  
Most Probable Number ◽  
Fish Feed ◽  
Internal Organs ◽  
Probable Number ◽  
Atlantic Salmon Salmo Salar ◽  
Gastric Intubation

The objective of our experiments was to study the persistence and dissemination of orally administered Salmonella in smoltified Atlantic salmon. In experiment 1, salmon kept at 15°C were fed for 1 week with feed contaminated with 96 most-probable-number units of Salmonella Agona per 100 g of feed and then starved for 2 weeks. Samples were taken from the gastrointestinal tract and examined for Salmonella 1, 2, 8, 9, 15, and 16 days after the feeding ended. In experiment 2, Salmonella Agona and Montevideo were separately mixed with feed and administered by gastric intubation. Each fish received 1.0 × 108, 1.0 × 106, or 1.0 × 104 CFU. The different groups were kept in parallel at 5 and 15°C and observed for 4 weeks. Every week, three fish in each group were sacrificed, and samples were taken from the skin, the pooled internal organs, the muscle, and the gastrointestinal tract and examined for the presence of Salmonella. The results from the two experiments showed that the persistence of Salmonella in the fish was highly dependent on the dose administered. Salmonella was not recovered from any of the fish that were fed for 1 week with the lowest concentration of Salmonella. In the fish given the highest dose of Salmonella, bacteria persisted for at least 4 weeks in the gastrointestinal tract as well as, to some extent, the internal organs. The present study shows that under practical conditions in Norway, the risk of Salmonella in fish feed being passed on to the consumer of the fish is negligible.

Experimental Salmonella enterica serovar Pullorum infection in two commercial varieties of laying hens

2001 ◽  
Vol 30 (2) ◽  
pp. 129-133 ◽  
Author(s):  
Lucas Augusto Soeiro Pinheiro ◽  
Gláucia Helaine De Oliveira ◽  
Angelo Berchieri
Keyword(s):  
Salmonella Enterica ◽  
Laying Hens ◽  
Salmonella Enterica Serovar Pullorum

Identification of Genes Responsible for Biofilm Formation or Virulence in Salmonella enterica Serovar Pullorum

2012 ◽  
Vol 56 (1) ◽  
pp. 134-143 ◽  
Author(s):  
Yan Lu ◽  
Sujuan Chen ◽  
Hongyan Dong ◽  
Hualu Sun ◽  
Daxin Peng ◽  
...  
Keyword(s):  
Salmonella Enterica ◽  
Biofilm Formation ◽  
Salmonella Enterica Serovar Pullorum

scholarly journals Salmonella enterica Serovar Pullorum Persists in Splenic Macrophages and in the Reproductive Tract during Persistent, Disease-Free Carriage in Chickens

2001 ◽  
Vol 69 (12) ◽  
pp. 7873-7879 ◽  
Author(s):  
P. Wigley ◽  
A. Berchieri ◽  
K. L. Page ◽  
A. L. Smith ◽  
P. A. Barrow
Keyword(s):  
Salmonella Enterica ◽  
Sexual Maturity ◽  
Reproductive Tract ◽  
Fluorescent Protein ◽  
Humoral Response ◽  
Main Site ◽  
Splenic Macrophages ◽  
Salmonella Enterica Serovar Pullorum ◽  
Green Fluorescent ◽  
Mechanisms Of Persistence

ABSTRACT Salmonella enterica serovar Pullorum is worldwide a poultry pathogen of considerable economic importance, particularly in those countries with a developing poultry industry. In addition to the characteristic high mortality rates among young chicks, one of the features of Salmonella serovar Pullorum infection is that it persists for long periods in convalescent chicks in the absence of clinical disease. This can lead to colonization of the reproductive tract of chickens and at sexual maturity can result in infected progeny through transovarian transmission to eggs. The sites ofSalmonella serovar Pullorum persistence in convalescent birds are not known, and the mechanisms of persistence are not understood. Here we show that Salmonella serovar Pullorum can persist in both the spleen and the reproductive tract for over 40 weeks following experimental infection in chickens. During the period of sexual maturity, Salmonella serovar Pullorum colonized both the ovary and the oviduct of hens and led to 6% of laid eggs being infected by Salmonella serovar Pullorum. The colonization of several different sites of the reproductive tract suggests that Salmonella serovar Pullorum may employ more than one mechanism of egg infection. Persistence occurred despite a strong humoral response, suggesting an intracellular site of infection. By use of a Salmonella serovar Pullorum strain containing a plasmid stably expressing green fluorescent protein, we demonstrated that the main site of carriage in the spleen is within macrophages. This raises interesting questions about the biology of Salmonella serovar Pullorum, including why there is an increase in bacterial numbers when birds become sexually mature and in particular how Salmonella serovar Pullorum avoids clearance by macrophages and whether it modulates the immune system in other ways.

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