Adaptation and growth kinetics study of an Indian isolate of virulent duck enteritis virus in Vero cells

2015 ◽  
Vol 78 ◽  
pp. 14-19 ◽  
Author(s):  
S. Aravind ◽  
Nitin M. Kamble ◽  
Satish S. Gaikwad ◽  
Sanjeev Kumar Shukla ◽  
Sohini Dey ◽  
...  
Keyword(s):  
Growth Kinetics ◽  
Vero Cells ◽  
Duck Enteritis Virus ◽  
Indian Isolate ◽  
Kinetics Study ◽  
Enteritis Virus

Proteomic analysis of host cellular proteins co-immunoprecipitated with duck enteritis virus gC

2021 ◽  
Vol 245 ◽  
pp. 104281
Author(s):  
Liu Chen ◽  
Zheng Ni ◽  
Jionggang Hua ◽  
Weicheng Ye ◽  
Keshu Liu ◽  
...  
Keyword(s):  
Proteomic Analysis ◽  
Duck Enteritis Virus ◽  
Cellular Proteins ◽  
Enteritis Virus

scholarly journals Development of a Colloidal Gold Immunochromatographic Assay for Duck Enteritis Virus Detection Using Monoclonal Antibodies

Pathogens ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 365
Author(s):  
Fengli Liu ◽  
Yanxin Cao ◽  
Maokai Yan ◽  
Mengxu Sun ◽  
Qingshui Zhang ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Colloidal Gold ◽  
Preventive Intervention ◽  
Virus Detection ◽  
Cross Reactivity ◽  
Duck Enteritis Virus ◽  
Detection Sensitivity ◽  
Immunochromatographic Assay ◽  
Efficient Detection ◽  
Enteritis Virus

Duck viral enteritis is a highly contagious and fatal disease of commercial waterfowl flocks. The disease occurs sporadically or epizootically in mainland China due to insufficient vaccinations. Early and rapid diagnosis is important for preventive intervention and the control of epizootic events in clinical settings. In this study, we generated two monoclonal antibodies (MAbs) that specifically recognized the duck enteritis virus (DEV) envelope glycoprotein B and tegument protein UL47, respectively. Using these MAbs, a colloidal gold-based immunochromatographic assay (ICA) was developed for the efficient detection of DEV antigens within 15 min. Our results showed that the detection limit of the developed ICA strip was 2.52 × 103 TCID50/mL for the virus infected cell culture suspension with no cross-reactivity with other pathogenic viruses commonly encountered in commercially raised waterfowl. Using samples from experimentally infected ducks, we demonstrated that the ICA detected the virus in cloacal swab samples on day three post-infection, demonstrating an 80% concordance with the PCR. For tissue homogenates from ducks succumbing to infection, the detection sensitivity was 100%. The efficient and specific detection by this ICA test provides a valuable, convenient, easy to use and rapid diagnostic tool for DVE under both laboratory and field conditions.

Recombinant duck enteritis virus expressing the HA gene from goose H5 subtype avian influenza virus

Vaccine ◽  
2013 ◽  
Vol 31 (50) ◽  
pp. 5953-5959 ◽  
Author(s):  
Xiaomei Liu ◽  
Shuangshi Wei ◽  
Yan Liu ◽  
Peifen Fu ◽  
Mingchun Gao ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Avian Influenza ◽  
Avian Influenza Virus ◽  
Duck Enteritis Virus ◽  
Ha Gene ◽  
H5 Subtype ◽  
Enteritis Virus

scholarly journals Different linkages in the long and short regions of the genomes of duck enteritis virus Clone-03 and VAC Strains

2011 ◽  
Vol 8 (1) ◽  
Author(s):  
Xiaoli Liu ◽  
Zongxi Han ◽  
Yuhao Shao ◽  
Yang Li ◽  
Huixin Li ◽  
...  
Keyword(s):  
Duck Enteritis Virus ◽  
Enteritis Virus ◽  
Virus Clone

scholarly journals Complete Genomic Sequence of Chinese Virulent Duck Enteritis Virus

2012 ◽  
Vol 86 (10) ◽  
pp. 5965-5965 ◽  
Author(s):  
Y. Wu ◽  
A. Cheng ◽  
M. Wang ◽  
Q. Yang ◽  
D. Zhu ◽  
...  
Keyword(s):  
Genomic Sequence ◽  
Duck Enteritis Virus ◽  
Complete Genomic Sequence ◽  
Enteritis Virus ◽  
Complete Genomic

Latency Sites and Reactivation of Duck Enteritis Virus

2002 ◽  
Vol 46 (2) ◽  
pp. 308-313 ◽  
Author(s):  
Samia Shawky ◽  
Karel A. Schat
Keyword(s):  
Duck Enteritis Virus ◽  
Enteritis Virus

Molecular and pathological characterization of duck enteritis virus in Egypt

2018 ◽  
Vol 66 (1) ◽  
pp. 217-224
Author(s):  
Mohamed El‐Tholoth ◽  
Mohamed F. Hamed ◽  
Ahmed A. Matter ◽  
Kamel I. Abou EL‐Azm
Keyword(s):  
Duck Enteritis Virus ◽  
Enteritis Virus

scholarly journals Virulent duck enteritis virus infected DEF cells generate a unique pattern of viral microRNAs and a novel set of host microRNAs

2018 ◽  
Vol 14 (1) ◽  
Author(s):  
Xianglong Wu ◽  
Renyong Jia ◽  
Jiakun Zhou ◽  
Mingshu Wang ◽  
Shun Chen ◽  
...  
Keyword(s):  
Duck Enteritis Virus ◽  
Unique Pattern ◽  
Enteritis Virus
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