S100A10 downregulation inhibits the phagocytosis of Cryptococcus neoformans by murine brain microvascular endothelial cells

2011 ◽  
Vol 51 (3) ◽  
pp. 96-100 ◽  
Author(s):  
Yuchong Chen ◽  
Jianghan Chen ◽  
Hai Wen ◽  
Pinghui Gao ◽  
Jun Wang ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Cryptococcus Neoformans ◽  
Microvascular Endothelial Cells ◽  
Brain Microvascular Endothelial Cells ◽  
Murine Brain ◽  
Microvascular Endothelial

scholarly journals Cryptococcus neoformans induces alterations in the cytoskeleton of human brain microvascular endothelial cells

2003 ◽  
Vol 52 (11) ◽  
pp. 961-970 ◽  
Author(s):  
Steven H. M. Chen ◽  
Monique F. Stins ◽  
Sheng-He Huang ◽  
Yu Hua Chen ◽  
K. J. Kwon-Chung ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Human Brain ◽  
Cryptococcus Neoformans ◽  
Microvascular Endothelial Cells ◽  
Brain Microvascular Endothelial Cells ◽  
Microvascular Endothelial

Adhesion molecules on murine brain microvascular endothelial cells: expression and regulation of ICAM-1 and Lgp 55

1992 ◽  
Vol 36 (1) ◽  
pp. 1-11 ◽  
Author(s):  
Zsuzsanna Fabry ◽  
Mari M. Waldschmidt ◽  
Dana Hendrickson ◽  
Judy Keiner ◽  
Laurie Love-Homan ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Adhesion Molecules ◽  
Microvascular Endothelial Cells ◽  
Brain Microvascular Endothelial Cells ◽  
Murine Brain ◽  
Microvascular Endothelial

HIV-1 gp41 ectodomain enhances Cryptococcus neoformans binding to human brain microvascular endothelial cells via gp41 core-induced membrane activities

2011 ◽  
Vol 438 (3) ◽  
pp. 457-466 ◽  
Author(s):  
Sheng-He Huang ◽  
Chu-Hua Wu ◽  
Shibo Jiang ◽  
Ingrid Bahner ◽  
Albert S. Lossinsky ◽  
...  
Keyword(s):  
Amino Acids ◽  
Endothelial Cells ◽  
Human Brain ◽  
Cryptococcus Neoformans ◽  
Microvascular Endothelial Cells ◽  
Brain Microvascular Endothelial Cells ◽  
Induced Membrane ◽  
Gp41 Ectodomain ◽  
Microvascular Endothelial ◽  
Hiv 1

Cryptococcus neoformans causes life-threatening meningoencephalitis, particularly prevalent in AIDS patients. The interrelationship between C. neoformans and HIV-1 is intriguing, as both pathogens elicit severe neuropathological complications. We have previously demonstrated that the HIV-1 gp41 ectodomain fragments gp41-I33 (amino acids 579–611) and gp41-I90 (amino acids 550–639) can enhance C. neoformans binding to HBMECs (human brain microvascular endothelial cells). Both peptides contain the loop region of gp41. In the present study, we used immunofluorescence microscopy and transmission and scanning electron microscopy to explore the underlying mechanisms. Our findings indicated that both C. neoformans and gp41-I90 up-regulated ICAM-1 (intercellular adhesion molecule 1) on the HBMECs and elicited membrane ruffling on the surface of HBMECs. The HIV-1 gp41 ectodomain could also induce CD44 and β-actin redistribution to the membrane lipid rafts, but it could not enhance PKCα (protein kinase Cα) phosphorylation like C. neoformans. Instead, gp41-I90 was able to induce syncytium formation on HBMECs. The results of the present study suggest HIV-1 gp41-enhanced C. neoformans binding to HBMECs via gp41 core domain-induced membrane activities, revealing a potential mechanism of invasion for this pathogenic fungus into the brain tissues of HIV-1-infected patients.

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