Identification and characterization of inosine 5-monophosphate dehydrogenase in Streptococcus suis type 2

2009 ◽  
Vol 47 (5) ◽  
pp. 267-273 ◽  
Author(s):  
Xue-han Zhang ◽  
Kong-wang He ◽  
Zhi-tao Duan ◽  
Jun-ming Zhou ◽  
Zheng-yu Yu ◽  
...  
Keyword(s):  
Streptococcus Suis ◽  
Identification And Characterization ◽  
Monophosphate Dehydrogenase

Lead identification and characterization of hTrkA type 2 inhibitors

2019 ◽  
Vol 29 (22) ◽  
pp. 126680 ◽  
Author(s):  
Govindan Subramanian ◽  
Yaqi Zhu ◽  
Scott J. Bowen ◽  
Nicole Roush ◽  
Julie A. White ◽  
...  
Keyword(s):  
Lead Identification ◽  
Identification And Characterization

Identification and characterization of a type-2 diacylglycerol acyltransferase (DGAT2) from Rhodosporidium diobovatum

2014 ◽  
Vol 106 (6) ◽  
pp. 1127-1137 ◽  
Author(s):  
Zhihuan Chen ◽  
Pengyan Liu ◽  
Yanhua Liu ◽  
Hui Tang ◽  
Yunping Chen ◽  
...  
Keyword(s):  
Diacylglycerol Acyltransferase ◽  
Rhodosporidium Diobovatum ◽  
Identification And Characterization

Identification and Characterization of Carbohydrate Molecules in Mammalian Cells Recognized by Dengue Virus Type 2

2006 ◽  
Vol 139 (3) ◽  
pp. 607-614 ◽  
Author(s):  
Chie Aoki ◽  
Kazuya I.P.J. Hidari ◽  
Saki Itonori ◽  
Akihiro Yamada ◽  
Naonori Takahashi ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Virus Type ◽  
Mammalian Cells ◽  
Identification And Characterization ◽  
Dengue Virus Type 2

scholarly journals Characterization of Streptococcus suis capsular type 2 haemolysin

Microbiology ◽  
1995 ◽  
Vol 141 (1) ◽  
pp. 189-195 ◽  
Author(s):  
M. G. Gottschalk ◽  
S. Lacouture ◽  
D. J.
Keyword(s):  
Streptococcus Suis ◽  
Capsular Type

scholarly journals Identification and Characterization of Shiga Toxin Type 2 Variants in Escherichia coli Isolates from Animals, Food, and Humans

2008 ◽  
Vol 74 (18) ◽  
pp. 5645-5652 ◽  
Author(s):  
Jie Zheng ◽  
Shenghui Cui ◽  
Louise D. Teel ◽  
Shaohua Zhao ◽  
Ruby Singh ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Shiga Toxin ◽  
Gene Encoding ◽  
Intestinal Mucus ◽  
One Step ◽  
A Subunit ◽  
Pcr Method ◽  
Identification And Characterization

ABSTRACT There is considerable heterogeneity among the Shiga toxin type 2 (Stx2) toxins elaborated by Shiga toxin-producing Escherichia coli (STEC). One such Stx2 variant, the Stx2d mucus-activatable toxin (Stx2dact), is rendered more toxic by the action of elastase present in intestinal mucus, which cleaves the last two amino acids of the A2 portion of the toxin A subunit. We screened 153 STEC isolates from food, animals, and humans for the gene encoding Stx2dact by using a novel one-step PCR procedure. This method targeted the region of stx 2dact that encodes the elastase recognition site. The presence of stx 2dact was confirmed by DNA sequencing of the complete toxin genes. Seven STEC isolates from cows (four isolates), meat (two isolates), and a human (one isolate) that carried the putative stx 2dact gene were identified; all were eae negative, and none was the O157:H7 serotype. Three of the isolates (CVM9322, CVM9557, and CVM9584) also carried stx 1, two (P1332 and P1334) carried stx 1 and stx 2c, and one (CL-15) carried stx 2c. One isolate, P1130, harbored only stx 2dact. The Vero cell cytotoxicities of supernatants from P1130 and stx 1 deletion mutants of CVM9322, CVM9557, and CVM9584 were increased 13- to 30-fold after treatment with porcine elastase. Thus, Stx2dact-producing strains, as detected by our one-step PCR method, can be isolated not only from humans, as previously documented, but also from food and animals. The latter finding has important public health implications based on a recent report from Europe of a link between disease severity and infection with STEC isolates that produce Stx2dact.

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