Molecular analysis of the hrp gene cluster in Xanthomonas oryzae pathovar oryzae KACC10859

2008 ◽  
Vol 44 (6) ◽  
pp. 473-483 ◽  
Author(s):  
Hee-Jung Cho ◽  
Young-Jin Park ◽  
Tae-Hwan Noh ◽  
Yeong-Tae Kim ◽  
Jeong-Gu Kim ◽  
...  
Keyword(s):  
Gene Cluster ◽  
Molecular Analysis ◽  
Xanthomonas Oryzae

Molecular analysis of the β-globin gene cluster haplotypes in a Sudanese population with sickle cell anaemia

2012 ◽  
Vol 34 (3) ◽  
pp. 262-266 ◽  
Author(s):  
A. Y. ELDERDERY ◽  
J. MILLS ◽  
B. A. MOHAMED ◽  
A. J. COOPER ◽  
A. O. MOHAMMED ◽  
...  
Keyword(s):  
Gene Cluster ◽  
Molecular Analysis ◽  
Sickle Cell ◽  
Sickle Cell Anaemia ◽  
Globin Gene ◽  
Globin Gene Cluster

scholarly journals Identification of Two Novelhrp-Associated Genes in the hrp Gene Cluster ofXanthomonas oryzae pv. oryzae

2000 ◽  
Vol 182 (7) ◽  
pp. 1844-1853 ◽  
Author(s):  
Weiguang Zhu ◽  
Mark M. MaGbanua ◽  
Frank F. White
Keyword(s):  
Nucleotide Sequence ◽  
Gene Cluster ◽  
Hypersensitive Response ◽  
Xanthomonas Campestris ◽  
Host Plants ◽  
Xanthomonas Oryzae ◽  
Insertion Element ◽  
Nonhost Plant ◽  
Rice Leaves ◽  
Glycine Rich Protein

ABSTRACT We have cloned a hrp gene cluster fromXanthomonas oryzae pv. oryzae. Bacteria with mutations in the hrp region have reduced growth in rice leaves and lose the ability to elicit a hypersensitive response (HR) on the appropriate resistant cultivars of rice and the nonhost plant tomato. A 12,165-bp portion of nucleotide sequence from the presumed left end and extending through the hrpB operon was determined. The region was most similar to hrp genes from Xanthomonas campestris pv. vesicatoria and Ralstonia solanacearum. Two new hrp-associated loci, namedhpa1 and hpa2, were located beyond thehrpA operon. The hpa1 gene encoded a 13-kDa glycine-rich protein with a composition similar to those of harpins and PopA. The product of hpa2 was similar to lysozyme-like proteins. Perfect PIP boxes were present in the hrpB andhpa1 operons, while a variant PIP box was located upstream of hpa2. A strain with a deletion encompassinghpa1 and hpa2 had reduced pathogenicity and elicited a weak HR on nonhost and resistant host plants. Experiments using single mutations in hpa1 and hpa2indicated that the loss of hpa1 was the principal cause of the reduced pathogenicity of the deletion strain. A 1,519-bp insertion element was located immediately downstream of hpa2. Hybridization with hpa2 indicated that the gene was present in all of the strains of Xanthomonas examined. Hybridization experiments with hpa1 and IS1114indicated that these sequences were detectable in all strains ofX. oryzae pv. oryzae and some other Xanthomonasspecies.

scholarly journals Molecular Analysis of the Kirromycin Biosynthetic Gene Cluster Revealed β-Alanine as Precursor of the Pyridone Moiety

2008 ◽  
Vol 15 (2) ◽  
pp. 175-188 ◽  
Author(s):  
Tilmann Weber ◽  
Kristina Juliane Laiple ◽  
Eva Karoline Pross ◽  
Adriana Textor ◽  
Stephanie Grond ◽  
...  
Keyword(s):  
Gene Cluster ◽  
Molecular Analysis ◽  
Biosynthetic Gene Cluster ◽  
Biosynthetic Gene

scholarly journals Molecular analysis of the cercosporin biosynthetic gene cluster in Cercospora nicotianae

2007 ◽  
Vol 64 (3) ◽  
pp. 755-770 ◽  
Author(s):  
Huiqin Chen ◽  
Miin-Huey Lee ◽  
Margret E. Daub ◽  
Kuang-Ren Chung
Keyword(s):  
Gene Cluster ◽  
Molecular Analysis ◽  
Biosynthetic Gene Cluster ◽  
Biosynthetic Gene
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