Burkholderia pseudomallei RpoS regulates multinucleated giant cell formation and inducible nitric oxide synthase expression in mouse macrophage cell line (RAW 264.7)

2006 ◽  
Vol 40 (4) ◽  
pp. 184-189 ◽  
Author(s):  
P. Utaisincharoen ◽  
S. Arjcharoen ◽  
K. Limposuwan ◽  
S. Tungpradabkul ◽  
S. Sirisinha
Keyword(s):  
Multinucleated Giant Cell ◽  
Burkholderia Pseudomallei ◽  
Cell Formation ◽  
Macrophage Cell Line ◽  
Macrophage Cell ◽  
Giant Cell Formation ◽  
Oxide Synthase ◽  
Multinucleated Giant Cell Formation ◽  
Mouse Macrophage Cell Line ◽  
Inducible Nitric Oxide

scholarly journals A role for tetraspanin proteins in regulating fusion induced by Burkholderia thailandensis

2020 ◽  
Vol 209 (4) ◽  
pp. 473-487 ◽  
Author(s):  
Atiga Elgawidi ◽  
Muslim Idan Mohsin ◽  
Fawwaz Ali ◽  
Amyleigh Watts ◽  
Peter N. Monk ◽  
...  
Keyword(s):  
Burkholderia Pseudomallei ◽  
Cell Formation ◽  
High Morbidity ◽  
Virulence Determinants ◽  
Macrophage Cell ◽  
Burkholderia Thailandensis ◽  
Bacterial Pathogenicity ◽  
Giant Cell Formation ◽  
Host Cell Factors ◽  
Multinucleated Giant Cell Formation

Abstract Burkholderia pseudomallei is the causative agent of melioidosis, a disease with high morbidity that is endemic in South East Asia and northern Australia. An unusual feature of the bacterium is its ability to induce multinucleated giant cell formation (MNGC), which appears to be related to bacterial pathogenicity. The mechanism of MNGC formation is not fully understood, but host cell factors as well as known bacterial virulence determinants are likely to contribute. Since members of the tetraspanin family of membrane proteins are involved in various types of cell:cell fusion, their role in MNGC formation induced by Burkholderia thailandensis, a mildly pathogenic species closely related to B. pseudomallei, was investigated. The effect of antibodies to tetraspanins CD9, CD81, and CD63 in MNGC formation induced by B. thailandensis in infected mouse J774.2 and RAW macrophage cell lines was assessed along with that of recombinant proteins corresponding to the large extracellular domain (EC2) of the tetraspanins. B. thailandensis-induced fusion was also examined in macrophages derived from CD9 null and corresponding WT mice, and in J774.2 macrophages over-expressing CD9. Antibodies to CD9 and CD81 promoted MNGC formation induced by B. thailandensis, whereas EC2 proteins of CD9, CD81, and CD63 inhibited MNGC formation. Enhanced MNGC formation was observed in CD9 null macrophages, whereas a decrease in MNGC formation was associated with overexpression of CD9. Overall our findings show that tetraspanins are involved in MNGC formation induced by B. thailandensis and by implication, B. pseudomallei, with CD9 and CD81 acting as negative regulators of this process.

Isatins inhibit cyclooxygenase-2 and inducible nitric oxide synthase in a mouse macrophage cell line

2007 ◽  
Vol 556 (1-3) ◽  
pp. 200-206 ◽  
Author(s):  
Maria Eline Matheus ◽  
Flávio de Almeida Violante ◽  
Simon John Garden ◽  
Angelo C. Pinto ◽  
Patricia Dias Fernandes
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Cell Line ◽  
Inducible Nitric Oxide Synthase ◽  
Mouse Macrophage ◽  
Macrophage Cell Line ◽  
Macrophage Cell ◽  
Oxide Synthase ◽  
Mouse Macrophage Cell Line ◽  
Inducible Nitric Oxide
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