Expression of firefly luciferase in Candida albicans and its use in the selection of stable transformants

2006 ◽  
Vol 40 (2) ◽  
pp. 69-81 ◽  
Author(s):  
Timothy C. Doyle ◽  
Kevin A. Nawotka ◽  
Anthony F. Purchio ◽  
Ali R. Akin ◽  
Kevin P. Francis ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Firefly Luciferase ◽  
Stable Transformants ◽  
Selection Of

Selection of potential anti-adhesion drugs by in silico approaches targeted to ALS3 from Candida albicans

2019 ◽  
Vol 41 (12) ◽  
pp. 1391-1401 ◽  
Author(s):  
Erika Seki Kioshima ◽  
Cristiane Suemi Shinobu-Mesquita ◽  
Ana Karina Rodrigues Abadio ◽  
Maria Sueli Soares Felipe ◽  
Terezinha Inez Estivalet Svidzinski ◽  
...  
Keyword(s):  
Candida Albicans ◽  
In Silico ◽  
Selection Of

Direct selection of galactokinase-negative mutants of Candida albicans using 2-deoxy-galactose

1992 ◽  
Vol 21 (3) ◽  
pp. 203-206 ◽  
Author(s):  
Jessica A. Gorman ◽  
John W Gorman ◽  
Y. Koltin
Keyword(s):  
Candida Albicans ◽  
Direct Selection ◽  
Selection Of

Anaerobic enhancement of protease secretion by periodontal Candida albicans strains

2013 ◽  
Vol 9 (2) ◽  
Author(s):  
Edvaldo Antonio Ribeiro Rosa ◽  
Alinne Ulbrich Mores Rymovicz ◽  
Rodrigo Nunes Rached ◽  
Ana Maria Trindade Grégio ◽  
Wander José da Silva ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Periodontal Disease ◽  
Molecular Oxygen ◽  
Relative Frequency ◽  
Aspartyl Protease ◽  
Atmospheric Conditions ◽  
Anoxic Conditions ◽  
Specific Protease ◽  
Protease Secretion ◽  
Selection Of

Introduction: Among other non-bacterial organisms, yeasts have been isolated from subgingival sites with relative frequency. Candida albicans is the species most commonly isolated although its role in periodontal disease has not been established. Objective: This study evaluated the secretion patterns of aspartyl-protease (Sap) by periodontal and nonperiodontal Candida albicans strains in normoxic and anoxic conditions. Material and methods: Periodontal strains (n=10; periodontal pockets ≥3.00 mm) and nonperiodontal Candida albicans strains (n=10) were grown under normoxic and anoxic conditions in protease-inducible broth. Sap activities were quantified in supernatants using azocasein as substrate. Whole-protein contents in supernatants were determined by Bradford’s method. Specific protease activities (Sap activity.protein-1) were assessed and compared. Results: While nonperiodontal strains secrete similar amounts of Sap under both atmospheric conditions, periodontal strains secrete reduced amounts in the presence of molecular oxygen. Conclusion: Despite the limited number of assayed isolates, the possibilities of adaptation or selection of candidal strains to periodontal microenvironment may be considered.

scholarly journals ALS3 Expression as an Indicator for Candida albicans Biofilm Formation and Drug Resistance

2021 ◽  
Vol 12 ◽  
Author(s):  
Keke Deng ◽  
Wei Jiang ◽  
Yanyu Jiang ◽  
Qi Deng ◽  
Jinzhong Cao ◽  
...  
Keyword(s):  
Gene Expression ◽  
Candida Albicans ◽  
Biofilm Formation ◽  
Antifungal Agents ◽  
Early Prevention ◽  
Therapy Failure ◽  
Prevention And Treatment ◽  
Selection Of ◽  
Prevention And Therapy

Resistance caused by the formation of the Candida albicans (C. albicans) biofilm is one of the main reasons for antifungal therapy failure. Thus, it is important to find indicators that predict C. albicans biofilm formation to provide evidence for the early prevention and treatment of the C. albicans biofilms. In this study, C. albicans samples were selected from C. albicans septicemia that were sensitive to common antifungal agents. It was found that the agglutinin-like sequence 3 (ALS3) gene was differentially expressed in free, antifungal, drug-sensitive C. albicans. The average ALS3 gene expression was higher in the C. albicans strains with biofilm formation than that in the C. albicans strains without biofilm formation. Then, it was further confirmed that the rate of biofilm formation was higher in the high ALS3 gene expression group than that in the low ALS3 gene expression group. It was found that C. albicans with biofilm formation was more resistant to fluconazole, voriconazole, and itraconazole. However, it maintained its sensitivity to caspofungin and micafungin in vitro and in mice. Further experiments regarding the prevention of C. albicans biofilm formation were performed in mice, in which only caspofungin and micafungin prevented C. albicans biofilm formation. These results suggest that the expression level of ALS3 in C. albicans may be used as an indicator to determine whether C. albicans will form biofilms. The results also show that the biofilm formation of C. albicans remained sensitive to caspofungin and micafungin, which may help to guide the selection of clinical antifungal agents for prevention and therapy.

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