Mouse intestinal innate immune responses altered by enterotoxigenic Escherichia coli (ETEC) infection

2014 ◽  
Vol 16 (11) ◽  
pp. 954-961 ◽  
Author(s):  
Wenkai Ren ◽  
Jie Yin ◽  
Jielin Duan ◽  
Gang Liu ◽  
Xiaoping Zhu ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Immune Responses ◽  
Innate Immune ◽  
Enterotoxigenic Escherichia Coli ◽  
Innate Immune Responses ◽  
Etec Infection

scholarly journals Enterotoxigenic Escherichia coli Prevents Host NF-κB Activation by Targeting IκBα Polyubiquitination

2012 ◽  
Vol 80 (12) ◽  
pp. 4417-4425 ◽  
Author(s):  
Xiaogang Wang ◽  
Philip R. Hardwidge
Keyword(s):  
Escherichia Coli ◽  
Immune Responses ◽  
Diarrheal Disease ◽  
Innate Immune ◽  
Host Cells ◽  
Enterotoxigenic Escherichia Coli ◽  
Host Responses ◽  
Innate Immune Responses ◽  
Content Type ◽  
Heat Stable

ABSTRACTThe NF-κB pathway regulates innate immune responses to infection. NF-κB is activated after pathogen-associated molecular patterns are detected, leading to the induction of proinflammatory host responses. As a countermeasure, bacterial pathogens have evolved mechanisms to subvert NF-κB signaling. EnterotoxigenicEscherichia coli(ETEC) causes diarrheal disease and significant morbidity and mortality for humans in developing nations. The extent to which this important pathogen subverts innate immune responses by directly targeting the NF-κB pathway is an understudied topic. Here we report that ETEC secretes a heat-stable, proteinaceous factor that blocks NF-κB signaling normally induced by tumor necrosis factor (TNF), interleukin-1β, and flagellin. Pretreating intestinal epithelial cells with ETEC supernatant significantly blocked the degradation of the NF-κB inhibitor IκBα without affecting IκBα phosphorylation. Data from immunoprecipitation experiments suggest that the ETEC factor functions by preventing IκBα polyubiquitination. Inhibiting clathrin function blocked the activity of the secreted ETEC factor, suggesting that this yet-uncharacterized activity may utilize clathrin-dependent endocytosis to enter host cells. These data suggest that ETEC evades the host innate immune response by directly modulating NF-κB signaling.

scholarly journals Zinc Influences Innate Immune Responses in Children with Enterotoxigenic Escherichia coli-Induced Diarrhea

2010 ◽  
Vol 140 (5) ◽  
pp. 1049-1056 ◽  
Author(s):  
Alaullah Sheikh ◽  
Sohel Shamsuzzaman ◽  
Shaikh Meshbahuddin Ahmad ◽  
Dilruba Nasrin ◽  
Setarun Nahar ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Immune Responses ◽  
Innate Immune ◽  
Enterotoxigenic Escherichia Coli ◽  
Innate Immune Responses

scholarly journals Immunogenicity and protective efficacy of enterotoxigenic Escherichia coli (ETEC) total RNA against ETEC challenge in a mouse model

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Mandi Liu ◽  
Yue Zhang ◽  
Di Zhang ◽  
Yun Bai ◽  
Guomei Liu ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Mouse Model ◽  
Immune Responses ◽  
Protective Efficacy ◽  
Enterotoxigenic Escherichia Coli ◽  
Economic Losses ◽  
Th2 Response ◽  
Total Rna ◽  
Etec Infection

AbstractEnterotoxigenic Escherichia coli (ETEC), an essential cause of post-weaning diarrhea (PWD) in piglets, leads to significant economic losses to the pig industry. The present study aims to identify the role of ETEC total RNA in eliciting immune responses to protect animals against ETEC infection. The results showed that the total RNA isolated from pig-derived ETEC K88ac strain effectively stimulated the IL-1β secretion of porcine intestinal epithelial cells (IPEC-J2). The mouse model immunized with ETEC total RNA via intramuscular injection (IM) or oral route (OR) was used to evaluate the protective efficiency of the ETEC total RNA. The results suggested that 70 μg ETEC total RNA administered by either route significantly promoted the production of the serum IL-1β and K88ac specific immunoglobulins (IgG, IgM, and IgA). Besides, the ETEC RNA administration augmented strong mucosal immunity by elevating K88ac specific IgA level in the intestinal fluid. Intramuscularly administered RNA induced a Th1/Th2 shift toward a Th2 response, while the orally administered RNA did not. The ETEC total RNA efficiently protected the animals against the ETEC challenge either by itself or as an adjuvant. The histology characterization of the small intestines also suggested the ETEC RNA administration protected the small intestinal structure against the ETEC infection. Particularly of note was that the immunity level and protective efficacy caused by ETEC RNA were dose-dependent. These findings will help understand the role of bacterial RNA in eliciting immune responses, and benefit the development of RNA-based vaccines or adjuvants.

scholarly journals Comparison of Holstein and Jersey Innate Immune Responses to Escherichia coli Intramammary Infection

2008 ◽  
Vol 91 (6) ◽  
pp. 2225-2235 ◽  
Author(s):  
D.D. Bannerman ◽  
A.C.W. Kauf ◽  
M.J. Paape ◽  
H.R. Springer ◽  
J.P. Goff
Keyword(s):  
Escherichia Coli ◽  
Immune Responses ◽  
Innate Immune ◽  
Innate Immune Responses ◽  
Intramammary Infection

Garlic and ginger extracts modulated broiler chicks innate immune responses and enhanced multidrug resistant Escherichia coli O78 clearance

2019 ◽  
Vol 66 ◽  
pp. 101334 ◽  
Author(s):  
Gamal A Elmowalid ◽  
Marwa I Abd El-Hamid ◽  
Ashraf M Abd El-Wahab ◽  
Mohammad Atta ◽  
Gamal Abd El-Naser ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Immune Responses ◽  
Multidrug Resistant ◽  
Innate Immune ◽  
Broiler Chicks ◽  
Innate Immune Responses

scholarly journals Escherichia coli and Staphylococcus aureus Elicit Differential Innate Immune Responses following Intramammary Infection

2004 ◽  
Vol 11 (3) ◽  
pp. 463-472 ◽  
Author(s):  
Douglas D. Bannerman ◽  
Max J. Paape ◽  
Jai-Wei Lee ◽  
Xin Zhao ◽  
Jayne C. Hope ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Immune Responses ◽  
Clinical Mastitis ◽  
Cytokine Response ◽  
Innate Immune ◽  
Host Recognition ◽  
Innate Immune Responses ◽  
E Coli ◽  
Intramammary Infection

ABSTRACTStaphylococcus aureusandEscherichia coliare among the most prevalent species of gram-positive and gram-negative bacteria, respectively, that induce clinical mastitis. The innate immune system comprises the immediate host defense mechanisms to protect against infection and contributes to the initial detection of and proinflammatory response to infectious pathogens. The objective of the present study was to characterize the different innate immune responses to experimental intramammary infection withE. coliandS. aureusduring clinical mastitis. The cytokine response and changes in the levels of soluble CD14 (sCD14) and lipopolysaccharide-binding protein (LBP), two proteins that contribute to host recognition of bacterial cell wall products, were studied. Intramammary infection with eitherE. coliorS. aureuselicited systemic changes, including decreased milk output, a febrile response, and induction of the acute-phase synthesis of LBP. Infection with either bacterium resulted in increased levels of interleukin 1β (IL-1β), gamma interferon, IL-12, sCD14, and LBP in milk. High levels of the complement cleavage product C5a and the anti-inflammatory cytokine IL-10 were detected at several time points followingE. coliinfection, whereasS. aureusinfection elicited a slight but detectable increase in these mediators at a single time point. Increases in IL-8 and tumor necrosis factor alpha were observed only in quarters infected withE. coli. Together, these data demonstrate the variability of the host innate immune response toE. coliandS. aureusand suggest that the limited cytokine response toS. aureusmay contribute to the well-known ability of the bacterium to establish chronic intramammary infection.

scholarly journals Uropathogenic Escherichia coli virulence and innate immune responses during urinary tract infection

2013 ◽  
Vol 16 (1) ◽  
pp. 100-107 ◽  
Author(s):  
Glen C Ulett ◽  
Makrina Totsika ◽  
Kolja Schaale ◽  
Alison J Carey ◽  
Matthew J Sweet ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Immune Responses ◽  
Uropathogenic Escherichia Coli ◽  
Innate Immune ◽  
Innate Immune Responses

Escherichia coli ghosts promote innate immune responses in human keratinocytes

2010 ◽  
Vol 400 (1) ◽  
pp. 78-82 ◽  
Author(s):  
Arby Abtin ◽  
Pavol Kudela ◽  
Ulrike Beate Mayr ◽  
Verena Juliana Koller ◽  
Michael Mildner ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Immune Responses ◽  
Human Keratinocytes ◽  
Innate Immune ◽  
Innate Immune Responses
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