Ultrastructural analysis of chlamydial antigen-containing vesicles everting from the Chlamydia trachomatis inclusion

2006 ◽  
Vol 8 (6) ◽  
pp. 1579-1591 ◽  
Author(s):  
David K. Giles ◽  
Judy D. Whittimore ◽  
Richard W. LaRue ◽  
Jane E. Raulston ◽  
Priscilla B. Wyrick
Keyword(s):  
Chlamydia Trachomatis ◽  
Ultrastructural Analysis ◽  
Chlamydial Antigen

Prevalence of Urogenital Chlamydia Trachomatis Infection in El Salvador. II. Gynaecology Outpatients

1992 ◽  
Vol 3 (6) ◽  
pp. 434-436 ◽  
Author(s):  
Ana Berta Cañas Posada ◽  
Jon Jonasson ◽  
Leonor de Linares ◽  
Solgun Bygdeman
Keyword(s):  
Chlamydia Trachomatis ◽  
El Salvador ◽  
Direct Immunofluorescence ◽  
Beta Lactamase ◽  
Gray Zone ◽  
Chlamydia Trachomatis Infection ◽  
San Salvador ◽  
Urogenital Infection ◽  
Healthy Women ◽  
Chlamydial Antigen

The prevalence of urogenital infection caused by Chlamydia trachomatis was examined in 100 non-pregnant women with cervicitis, and 100 healthy women, in San Salvador City, El Salvador. Pharmacia Chlamydia EIA test was used for the detection of chlamydial antigen in urethral and cervical specimens from all the women. Direct immunofluorescence was used for confirmative tests on the EIA positive and the negative gray zone samples. C. trachomatis antigen was detected in 28% of the women with cervicitis compared with 5% in the group of healthy women ( P < 0.001). The cervicitis group were also screened for Neisseria gonorrhoeae which was isolated from 12% of them. One strain out of 12 was beta-lactamase producing (PPNG). Five per cent of the women with cervicitis had simultaneous C. trachomatis and N. gonorrhoeae infections.

Ultrastructural analysis of the growth cycle of Chlamydia trachomatis in mouse cells treated with recombinant human α-interferons

1984 ◽  
Vol 41 (2) ◽  
pp. 227-235 ◽  
Author(s):  
Luis M. de la Maza ◽  
Jutta M. Goebel ◽  
Christine W. Czarniecki ◽  
Ellena M. Peterson
Keyword(s):  
Chlamydia Trachomatis ◽  
Growth Cycle ◽  
Ultrastructural Analysis ◽  
Mouse Cells

scholarly journals The microbicidal agent C31G inhibits Chlamydia trachomatis infectivity in vitro.

1997 ◽  
Vol 41 (6) ◽  
pp. 1335-1344 ◽  
Author(s):  
P B Wyrick ◽  
S T Knight ◽  
D G Gerbig ◽  
J E Raulston ◽  
C H Davis ◽  
...  
Keyword(s):  
Chlamydia Trachomatis ◽  
Epithelial Cell ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Independent Method ◽  
Epithelial Surface ◽  
Host Epithelial Cell ◽  
Chlamydial Inclusion ◽  
Chlamydial Antigen

Safe and effective vaginal microbicidal compounds are being sought to offer women an independent method for protection against transmission of sexually acquired pathogens. The purpose of this study was to examine the efficacy of two formulations of one such compound, C31G, against Chlamydia trachomatis serovar E alone, its host epithelial cell (HEC-1B) alone, and against chlamydiae-infected HEC-1B cells. Preexposure of isolated, purified infectious chlamydial elementary bodies (EB) to C31G, at pHs 7.2 and 5.7, for 1 h at 4 degrees C resulted in reduced infectivity of EB for HEC-1B cells. Examination of the C31G-exposed 35S-EB on sodium dodecyl sulfate-polyacrylamide gel electrophoresis autoradiographs and by Western blotting revealed a C31G concentration-dependent and pH-dependent destabilization of the chlamydial envelope, resulting in the release of chlamydial lipopolysaccharide and proteins. Interestingly, when the host human genital columnar epithelial cells were infected with chlamydiae and then exposed to dilute concentrations of C31G which did not alter epithelial cell viability, chlamydial infectivity was also markedly reduced. C31G gained access to the developing chlamydial inclusion causing damage to or destruction of metabolically active reticulate bodies as well as apparent alteration of the inclusion membrane, which resulted in premature escape of chlamydial antigen to the infected epithelial surface. These studies show that the broad-spectrum antiviral and antibacterial microbicide C31G also has antichlamydial activity.

Prevalence of Urogenital Chlamydia Trachomatis Infection in El Salvador I. Infection during Pregnancy and Perinatal Transmission

1992 ◽  
Vol 3 (1) ◽  
pp. 33-37 ◽  
Author(s):  
Ana Berta Cañas Posada ◽  
Jon Jonasson ◽  
Leonor de Linares ◽  
Solgun Bygdeman
Keyword(s):  
Chlamydia Trachomatis ◽  
Pregnant Women ◽  
El Salvador ◽  
Chlamydial Infection ◽  
Perinatal Transmission ◽  
Direct Immunofluorescence ◽  
Chlamydia Trachomatis Infection ◽  
San Salvador ◽  
Age Range ◽  
Chlamydial Antigen

One-hundred and twenty-nine pregnant women in labour (age range 15–46 years; median age 23) and 42 infants born to chlamydia-positive mothers (age range 5–15 days; median age 10) were investigated to estimate the prevalence and incidence, respectively, of Chlamydia trachomatis infection in San Salvador, El Salvador. Urethral and cervical samples were obtained from all women and conjunctival specimens were taken from both eyes of each child. The chlamydial antigen was detected with the commercial Pharmacia Chlamydia EIA kit. Direct immunofluorescence (DFA) (Syva MicroTrak) was used for confirmation. In the newborns both EIA and DFA tests on direct preparations from ocular smears were performed on all the samples. The prevalence of chlamydial infection in pregnant women was 44% (57/129). The incidence of chlamydial infection in neonates was 64% (27/42), and the majority of the infected children (56%) had conjunctivitis. Referring to individuals rather than specimens the sensitivity of EIA tests on conjunctival samples from the infants was low (37%) as compared with 91% on urethral and cervical specimens from the pregnant women.

Contributions to the mechanisms of mitosis and roles of cytoplasmic microtubules from ultrastructural analyses of fungi

1978 ◽  
Vol 36 (2) ◽  
pp. 266-267
Author(s):  
I. Brent Heath
Keyword(s):  
Nuclear Envelope ◽  
Direct Application ◽  
Ultrastructural Analysis ◽  
General Interest ◽  
Research Results ◽  
Organelle Motility ◽  
Cytoplasmic Microtubules

Detailed ultrastructural analysis of fungal mitotic systems and cytoplasmic microtubules might be expected to contribute to a number of areas of general interest in addition to the direct application to the organisms of study. These areas include possibly fundamental general mechanisms of mitosis; evolution of mitosis; phylogeny of organisms; mechanisms of organelle motility and positioning; characterization of cellular aspects of microtubule properties and polymerization control features. This communication is intended to outline our current research results relating to selected parts of the above questions.Mitosis in the oomycetes Saprolegnia and Thraustotheca has been described previously. These papers described simple kinetochores and showed that the kineto- chores could probably be used as markers for the poorly defined chromosomes. Kineto- chore counts from serially sectioned prophase mitotic nuclei show that kinetochore replication precedes centriole replication to yield a single hemispherical array containing approximately the 4 n number of kinetochore microtubules diverging from the centriole associated "pocket" region of the nuclear envelope (Fig. 1).

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