Homologous series of novel adamantane–colchicine conjugates: synthesis and cytotoxic effect on human cancer cells

2018 ◽  
Vol 28 (3) ◽  
pp. 308-310 ◽  
Author(s):  
Nikolay A. Zefirov ◽  
Marius Hoppe ◽  
Irina V. Kuznetsova ◽  
Nikita A. Chernyshov ◽  
Yuri K. Grishin ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Homologous Series ◽  
Cytotoxic Effect ◽  
Human Cancer ◽  
Human Cancer Cells

scholarly journals Effect of Calomelanone, a Dihydrochalcone Analogue, on Human Cancer Apoptosis/Regulated Cell Death in an In Vitro Model

2020 ◽  
Vol 2020 ◽  
pp. 1-16
Author(s):  
Wasitta Rachakhom ◽  
Ratana Banjerdpongchai
Keyword(s):  
Cell Death ◽  
Cancer Cells ◽  
Hepg2 Cells ◽  
Cytotoxic Effect ◽  
Human Cancer ◽  
Autophagic Flux ◽  
Dependent Manner ◽  
Anticancer Activities ◽  
Human Cancer Cells ◽  
Regulated Cell Death

Calomelanone, 2 ′ ,6 ′ -dihydroxy-4,4 ′ -dimethoxydihydrochalcone, possesses anticancer activities. This study was conducted to investigate the cytotoxic effect of calomelanone, a dihydrochalcone analogue, on human cancer cells and its associated mechanisms. The cytotoxic effect of calomelanone was measured by MTT assay. Annexin V-FITC/propidium iodide and DiOC6 staining that employed flow cytometry were used to determine the mode of cell death and reduction of mitochondrial transmembrane potential (MTP), respectively. Caspase activities were measured using specific substrates and colorimetric analysis. The expression levels of Bcl-2 family proteins were determined by immunoblotting. Reactive oxygen species were also measured using 2 ′ ,7 ′ -dihydrodichlorofluorescein diacetate and dihydroethidium (fluorescence dyes). Calomelanone was found to be toxic towards various human cancer cells, including acute promyelocytic HL-60 and monocytic leukemic U937 cells, in a dose-dependent manner at 24 h and human hepatocellular HepG2 cells at 48 h. However, the proliferation of HepG2 cells increased at 24 h. Calomelanone was found to induce apoptosis in HL-60 and U937 at 24 h and HepG2 apoptosis at 48 h via the intrinsic pathway by inducing MTP disruption. This compound also induced caspase-3, caspase-8, and caspase-9 activities. Calomelanone upregulated proapoptotic Bax and Bak and downregulated antiapoptotic Bcl-xL proteins in HepG2 cells. Moreover, signaling was also associated with oxidative stress in HepG2 cells. Calomelanone induced autophagy at 24 h of treatment, which was evidenced by staining with monodansylcadaverine (MDC) to represent autophagic flux. This was associated with a decrease of Akt (survival pathway) and an upregulation of Atg5 (the marker of autophagy). Thus, calomelanone induced apoptosis/regulated cell death in HL-60, U937, and HepG2 cells. However, it also induced autophagy in HepG2 depending on duration, dose, and type of cells. Thus, calomelanone could be used as a potential anticancer agent for cancer treatment. Nevertheless, acute and chronic toxicity should be further investigated in animals before conducting investigations in human patients.

Cytotoxic Effect of Zostera asiatica on Growth of Human Cancer Cells

KSBB Journal ◽  
2012 ◽  
Vol 27 (4) ◽  
pp. 227-231 ◽  
Author(s):  
Joo-Wan Hong ◽  
Myung-Eun Jung ◽  
Jung-Im Lee ◽  
Ho-Jun Kim ◽  
Jae-Soo Chang ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Cytotoxic Effect ◽  
Human Cancer ◽  
Human Cancer Cells

scholarly journals Bioactive Compound Content and Cytotoxic Effect on Human Cancer Cells of Fresh and Processed Yellow Tomatoes

Molecules ◽  
2015 ◽  
Vol 21 (1) ◽  
pp. 33 ◽  
Author(s):  
Assunta Raiola ◽  
Rita Del Giudice ◽  
Daria Monti ◽  
Gian Tenore ◽  
Amalia Barone ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Cytotoxic Effect ◽  
Human Cancer ◽  
Bioactive Compound ◽  
Human Cancer Cells

Cytotoxic effect of Berberis libanotica roots extracts on human cancer cells and antioxidant activities

2017 ◽  
Vol 6 (6) ◽  
pp. 311-317
Author(s):  
Kamar Hamade ◽  
◽  
Louna Karam ◽  
Jean Habib ◽  
Raghida Abou Merhi ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Cancer Cells ◽  
Cytotoxic Effect ◽  
Antioxidant Activities ◽  
Human Cancer ◽  
Medicinal Tree ◽  
Human Cancer Cells ◽  
Promising Source ◽  
Ht 29 ◽  
Mcf 7

In the present study, we evaluated the cytotoxic effect and the antioxidant activity of methanolic and ethanolic roots extracts obtained from Berberis libanotica a Lebanese medicinal tree. Cytotoxic activity was assessed on the colon cancer HT 29, HCT 116, Caco-2 and breast cancer MCF-7 and MDA-MB-231cell lines, using the MTT viability assay. Both extracts inhibited cancer cells proliferation in a doseand time depending manner without being cytotoxic against the normal MCF-10 cell line. Our results suggest that methanolic extract could induce a caspase-independent cell death in the colon and breast tumor cells HT 29 and MCF-7, respectively. DPPH and FRAP assays showed a moderate to strong antioxidant activity of the methanolic and ethanolic extracts with EC50 values of 0.13 ± 0.001 and 0.1± 0.002 mg/ml, respectively. Collectively, these findings suggest that Berberis libanotica roots could serve as a promising source of antioxidant and anticancer bioactive compounds.

scholarly journals Cytotoxic effect induced by combination of polyamines metabolites and endocannabinoid, anandamide, on human cancer cells: a new anticancer strategy

2010 ◽  
Vol 4 (S2) ◽  
Author(s):  
Ana Batista-de-Carvalho ◽  
Giampiero Tempera ◽  
Nikenza Viceconte ◽  
Stefania Saccoccio ◽  
Eris Bidollari ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Cytotoxic Effect ◽  
Human Cancer ◽  
Human Cancer Cells

Abstract 3318: Cytotoxic effect of photosensitive fluorescent protein KillerRed-expressing adenovirus against human cancer cells.

2013 ◽  
Author(s):  
Kiyoto Takehara ◽  
Hiroshi Tazawa ◽  
Yuuri Hashimoto ◽  
Hiroyuki Kishimoto ◽  
Nobuhiro Narii ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Cytotoxic Effect ◽  
Fluorescent Protein ◽  
Human Cancer ◽  
Human Cancer Cells
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