scholarly journals Transthyretin uptake in placental cells is regulated by the high-density lipoprotein receptor, scavenger receptor class B member 1

2018 ◽  
Vol 474 ◽  
pp. 89-96 ◽  
Author(s):  
Kelly A. Landers ◽  
Huika Li ◽  
Robin H. Mortimer ◽  
Donald S.A. McLeod ◽  
Michael C. d’Emden ◽  
...  
Keyword(s):  
High Density Lipoprotein ◽  
Scavenger Receptor ◽  
Density Lipoprotein ◽  
High Density ◽  
Lipoprotein Receptor ◽  
Scavenger Receptor Class ◽  
Placental Cells ◽  
Class B ◽  
Density Lipoprotein Receptor

scholarly journals Scavenger Receptor Class B Member 1 Independent Uptake of Transthyretin by Cultured Hepatocytes Is Regulated by High Density Lipoprotein

2019 ◽  
Vol 2019 ◽  
pp. 1-7
Author(s):  
Kelly A. Landers ◽  
Michael C. d’Emden ◽  
Kerry Richard
Keyword(s):  
High Density Lipoprotein ◽  
Scavenger Receptor ◽  
Density Lipoprotein ◽  
Cell Types ◽  
Normal Function ◽  
High Density ◽  
Scavenger Receptor Class ◽  
Class B ◽  
Density Lipoprotein Receptor ◽  
And Function

Thyroid hormone (thyroxine, T4) is essential for the normal function of all cell types and is carried in serum bound to several proteins including transthyretin. Recently, evidence has emerged of alternate pathways for hormone entry into cells that are dependent on hormone binding proteins. Transthyretin and transthyretin bound T4 are endocytosed by placental trophoblasts through the high-density lipoprotein receptor, Scavenger Receptor Class B Type 1 (SR-B1). High density lipoprotein (HDL) affects the expression and function of SR-B1 in trophoblast cells. SR-B1 is also expressed in hepatocytes and we sought to determine if hepatocyte SR-B1 was involved in transthyretin or transthyretin-T4 uptake and whether uptake was affected by HDL. Transthyretin and transthyretin-T4 uptake by hepatocytes is not dependent on SR-B1. HDL treatment reduced SR-B1 expression. However, pretreatment of hepatocytes with HDL increased uptake of transthyretin-T4. Knockdown of SR-B1 expression using siRNA also increased transthyretin-T4 uptake. Coaddition of HDL to transthyretin uptake experiments blocked both transthyretin and transthyretin-T4 uptake. Hepatocyte uptake of transthyretin-T4 uptake is influenced by, but is not dependent on, SR-B1 expression. HDL also decreases transthyretin-T4 uptake and therefore diet or drugs may interfere with this process. This suggests that multiple lipoprotein receptors may be involved in the regulation of uptake of transthyretin-T4 in a cell-type specific manner. Further study is required to understand this important process.

scholarly journals Binding of High Density Lipoprotein (HDL) and Discoidal Reconstituted HDL to the HDL Receptor Scavenger Receptor Class B Type I

2000 ◽  
Vol 275 (28) ◽  
pp. 21262-21271 ◽  
Author(s):  
Kalliopi N. Liadaki ◽  
Tong Liu ◽  
Shangzhe Xu ◽  
Brian Y. Ishida ◽  
Philippe N. Duchateaux ◽  
...  
Keyword(s):  
High Density Lipoprotein ◽  
Scavenger Receptor ◽  
Density Lipoprotein ◽  
High Density ◽  
Type I ◽  
Scavenger Receptor Class ◽  
Class B
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