scholarly journals 17β-Estradiol alters oxidative damage and oxidative stress response protein expression in the mouse mammary gland

2016 ◽  
Vol 426 ◽  
pp. 11-21 ◽  
Author(s):  
Lisi Yuan ◽  
Alicia K. Dietrich ◽  
Yvonne S. Ziegler ◽  
Ann M. Nardulli
Keyword(s):  
Oxidative Stress ◽  
Mammary Gland ◽  
Stress Response ◽  
Oxidative Damage ◽  
Protein Expression ◽  
Mouse Mammary Gland ◽  
Oxidative Stress Response ◽  
17Β Estradiol ◽  
And Oxidative Stress

Biological role, protein expression, subcellular localization, and oxidative stress response of paraoxonase 2 in the intestine of humans and rats

2007 ◽  
Vol 293 (6) ◽  
pp. G1252-G1261 ◽  
Author(s):  
Emile Levy ◽  
Karine Trudel ◽  
Moise Bendayan ◽  
Ernest Seidman ◽  
Edgard Delvin ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Stress Response ◽  
Subcellular Localization ◽  
Protein Expression ◽  
Oxidative Stress Response ◽  
Butylated Hydroxytoluene ◽  
Cell Fractionation ◽  
Rat Intestine ◽  
And Oxidative Stress

Oxidative stress is a cardinal manifestation of various intestinal disorders. However, very little knowledge is available on the intestine's inherent defense mechanisms against free radicals. This study was designed to determine the protein expression, subcellular localization and oxidative stress response of paraoxonase 2 (PON2), a member of a powerful antioxidant family in human and rat intestine. Biochemical and ultrastructural experiments all showed a substantial expression of PON2 in human and rat intestine. Western blot analysis disclosed higher levels of PON2 in the jejunum than in the duodenum, ileum, and colon. Cell fractionation revealed a predominant PON2 association with microsomes and lysosomes in the human jejunum, which differed from that in rats. PON2 was detected in the intestine as early as week 15 of gestation and was significantly increased by week 20. Iron ascorbate-mediated lipid peroxidation induced a marked decrease in PON2 expression in intestinal specimens coincidental to an abundant rise in malondialdehyde (MDA). On the other hand, preincubation with potent antioxidants, such as butylated hydroxytoluene, Trolox, and N-acetylcysteine, prevented iron-ascorbate-generating PON2 reduction in parallel with MDA suppression. Finally, the preincubation of permeabilized Caco-2 cells with purified PON2 led to a protection against iron-ascorbate-induced lipid peroxidation. These observations demonstrate that the human intestine is preferentially endowed with a marked PON2 expression compared with the rat intestine and this expression shows a developmental and intracellular pattern of distribution. Furthermore, our observations suggest PON2 protective effects against prooxidant stimuli in the small intestine.

Metformin inhibits the inflammatory and oxidative stress response induced by skin UVB-irradiation and provides 4-hydroxy-2-nonenal and nitrotyrosine formation and p53 protein activation

2020 ◽  
Vol 100 (2) ◽  
pp. 152-155
Author(s):  
Fernando Pinheiro Souza-Neto ◽  
Poliana Camila Marinello ◽  
Gabriela Pasqual Melo ◽  
Leandra Zambeli Naira Ramalho ◽  
Eliana M. Cela ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Stress Response ◽  
P53 Protein ◽  
Oxidative Stress Response ◽  
Uvb Irradiation ◽  
Protein Activation ◽  
And Oxidative Stress
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