In vitro interactions between mammary fibroblasts (Hs 578Bst) and cancer epithelial cells (MCF-7) modulate aromatase, steroid sulfatase and 17β-hydroxysteroid dehydrogenases

2015 ◽  
Vol 412 ◽  
pp. 339-348 ◽  
Author(s):  
Xiaoqiang Wang ◽  
Xiaoye Sang ◽  
Caroline Diorio ◽  
Sheng-Xiang Lin ◽  
Charles J. Doillon
Keyword(s):  
Epithelial Cells ◽  
Steroid Sulfatase ◽  
Hydroxysteroid Dehydrogenases ◽  
Mcf 7 ◽  
In Vitro Interactions

In vitro interactions between epithelial cells and Gyrodactylus derjavini

2000 ◽  
Vol 74 (3) ◽  
pp. 203-208 ◽  
Author(s):  
K. Buchmann ◽  
C.V. Nielsen ◽  
J. Bresciani
Keyword(s):  
Tissue Culture ◽  
Epithelial Cells ◽  
Cell Cultures ◽  
Culture Medium ◽  
Epithelioma Papulosum Cyprini ◽  
Skin Responses ◽  
Enzyme Reactivity ◽  
In Vitro Interactions

AbstractSkin responses of fish to various parasites have been shown to involve various immunologically competent cells producing factors which guide the reactions of epithelial cells. However, the present study has demonstrated that a monoculture of epithelial cells has the ability to encapsulate and partially degrade ectoparasites without involvement of leukocytes. The ectoparasitic monogeneanGyrodactylus derjavini was kept on a monolayer of Epithelioma Papulosum Cyprini (EPC) cells in 24-well multidishes supplied with tissue culture medium. Gyrodactylus derjavini did not reproduce but survived an incubation period of up to139 h in the system. Due to sterile conditions, dead gyrodactylids were not subjected to microbial degradation and remained intact for several weeks. However, at 40 days G. derjavini was overgrown by EPC-cells and became partly degraded during the following 15 days. Analysis of enzyme reactivity in EPC-cells showed reactions for ten enzymes including esterases, amidases, phosphatases and phosphohydrolases. No marked differences for the ten enzymes between cell cultures with and without the ectoparasites were found but it cannot be excluded that some of these enzymes took part in parasite degradation. The study showed the in vitro capability of epithelial cells to interact, encapsulate and degrade G. derjavini without the involvement of leukocytes. This response probably is non-specific and will not exclude that various immunocompetent cells and their products normally optimize and accelerate elimination of invading parasites in vivo.

In vitro Interactions of Endometrial Stromal and Epithelial Cells in Matrigel: Reorganization of the Extracellular Matrix

Pathobiology ◽  
1994 ◽  
Vol 62 (2) ◽  
pp. 104-108 ◽  
Author(s):  
Helmut Hopfer ◽  
Clifford A. Rinehart Jr ◽  
Günter Vollmer ◽  
David G. Kaufman
Keyword(s):  
Extracellular Matrix ◽  
Epithelial Cells ◽  
In Vitro Interactions

In vitro interactions between human breast cancer cells MCF-7 and human blood platelets

1987 ◽  
Vol 47 (6) ◽  
pp. 693-698 ◽  
Author(s):  
Joseph Abecassis ◽  
Alain Beretz ◽  
Régine Millon-Collard ◽  
Jean-Pierre Fricker ◽  
Michel Fber ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Human Breast Cancer ◽  
Breast Cancer Cells ◽  
Blood Platelets ◽  
Human Breast Cancer Cells ◽  
Human Breast ◽  
Human Blood Platelets ◽  
Mcf 7 ◽  
In Vitro Interactions

In vitro interactions of cryopreserved stallion spermatozoa and oviduct (uterine tube) epithelial cells or their secretory products

1999 ◽  
Vol 56 (1) ◽  
pp. 51-65 ◽  
Author(s):  
Joanna E. Ellington ◽  
Juan C. Samper ◽  
Allison E. Jones ◽  
Sylvia A. Oliver ◽  
Katherine M. Burnett ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Uterine Tube ◽  
Secretory Products ◽  
In Vitro Interactions

Electron probe x-ray microanalysis and electron microscopy of amino acid absorption and transport by the small intestine: inhibition by chemical poisons and correlation to the elemental composition of the epithelial cells

1986 ◽  
Vol 44 ◽  
pp. 134-135
Author(s):  
A. J. Tousimis
Keyword(s):  
Small Intestine ◽  
Amino Acid ◽  
Epithelial Cells ◽  
Elemental Composition ◽  
Isotope Labeling ◽  
Structural Components ◽  
X Ray ◽  
Human Small Intestine ◽  
Transport Studies

The elemental composition of amino acids is similar to that of the major structural components of the epithelial cells of the small intestine and other tissues. Therefore, their subcellular localization and concentration measurements are not possible by x-ray microanalysis. Radioactive isotope labeling: I131-tyrosine, Se75-methionine and S35-methionine have been successfully employed in numerous absorption and transport studies. The latter two have been utilized both in vitro and vivo, with similar results in the hamster and human small intestine. Non-radioactive Selenomethionine, since its absorption/transport behavior is assumed to be the same as that of Se75- methionine and S75-methionine could serve as a compound tracer for this amino acid.

Human thymic epithelial cells maintain long-term survival of clonogenic myeloid and erythroid progenitor cells in vitro

2000 ◽  
Vol 111 (1) ◽  
pp. 363-370 ◽  
Author(s):  
Katsuto Takenaka ◽  
Mine Harada ◽  
Tomoaki Fujisaki ◽  
Koji Nagafuji ◽  
Shinichi Mizuno ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Progenitor Cells ◽  
Thymic Epithelial Cells ◽  
Erythroid Progenitor ◽  
Term Survival ◽  
Long Term Survival ◽  
Erythroid Progenitor Cells
Sign in / Sign up

Export Citation Format

Share Document