scholarly journals Pulsed electromagnetic fields promote osteoblast mineralization and maturation needing the existence of primary cilia

2015 ◽  
Vol 404 ◽  
pp. 132-140 ◽  
Author(s):  
Juan-Li Yan ◽  
Jian Zhou ◽  
Hui-Ping Ma ◽  
Xiao-Ni Ma ◽  
Yu-Hai Gao ◽  
...  
Keyword(s):  
Electromagnetic Fields ◽  
Primary Cilia ◽  
Pulsed Electromagnetic Fields ◽  
Pulsed Electromagnetic

scholarly journals Exposure to 16 Hz Pulsed Electromagnetic Fields Protect the Structural Integrity of Primary Cilia and Associated TGF-β Signaling in Osteoprogenitor Cells Harmed by Cigarette Smoke

2021 ◽  
Vol 22 (13) ◽  
pp. 7036
Author(s):  
Yangmengfan Chen ◽  
Romina H. Aspera-Werz ◽  
Maximilian M. Menger ◽  
Karsten Falldorf ◽  
Michael Ronniger ◽  
...  
Keyword(s):  
Cell Viability ◽  
Cigarette Smoke ◽  
Electromagnetic Fields ◽  
Primary Cilia ◽  
Structural Integrity ◽  
Immunofluorescent Staining ◽  
Pulsed Electromagnetic Fields ◽  
Migration Assay ◽  
Daily Exposure ◽  
Pulsed Electromagnetic

Cigarette smoking (CS) is one of the main factors related to avoidable diseases and death across the world. Cigarette smoke consists of numerous toxic compounds that contribute to the development of osteoporosis and fracture nonunion. Exposure to pulsed electromagnetic fields (PEMF) was proven to be a safe and effective therapy to support bone fracture healing. The aims of this study were to investigate if extremely low frequency (ELF-) PEMFs may be beneficial to treat CS-related bone disease, and which effect the duration of the exposure has. In this study, immortalized human mesenchymal stem cells (SCP-1 cells) impaired by 5% cigarette smoke extract (CSE) were exposed to ELF-PEMFs (16 Hz) with daily exposure ranging from 7 min to 90 min. Cell viability, adhesion, and spreading were evaluated by Sulforhodamine B, Calcein-AM staining, and Phalloidin-TRITC/Hoechst 33342 staining. A migration assay kit was used to determine cell migration. Changes in TGF-β signaling were evaluated with an adenoviral Smad2/3 reporter assay, RT-PCR, and Western blot. The structure and distribution of primary cilia were analyzed with immunofluorescent staining. Our data indicate that 30 min daily exposure to a specific ELF-PEMF most effectively promoted cell viability, enhanced cell adhesion and spreading, accelerated migration, and protected TGF-β signaling from CSE-induced harm. In summary, the current results provide evidence that ELF-PEMF can be used to support early bone healing in patients who smoke.

Effect of pulsed electromagnetic fields on maturation of regenerate bone in a rabbit limb lengthening model

2005 ◽  
Vol 24 (1) ◽  
pp. 2-10 ◽  
Author(s):  
Kenneth F. Taylor ◽  
Nozumu Inoue ◽  
Bahman Rafiee ◽  
John E. Tis ◽  
Kathleen A. McHale ◽  
...  
Keyword(s):  
Electromagnetic Fields ◽  
Limb Lengthening ◽  
Pulsed Electromagnetic Fields ◽  
Pulsed Electromagnetic

Effects of Pulsed Electromagnetic Fields on Rat Eye

1999 ◽  
Vol 18 (2) ◽  
pp. 157-164
Author(s):  
Gianluca Giavaresi ◽  
Francesco Broccoli ◽  
Milena Fini ◽  
Paola Torricelli ◽  
Paola Versura ◽  
...  
Keyword(s):  
Electromagnetic Fields ◽  
Pulsed Electromagnetic Fields ◽  
Pulsed Electromagnetic

scholarly journals Effects of pulsed electromagnetic fields on cartilage apoptosis signalling pathways in ovariectomised rats

2011 ◽  
Vol 35 (12) ◽  
pp. 1875-1882 ◽  
Author(s):  
Shasha Li ◽  
Qinglu Luo ◽  
Liqun Huang ◽  
Yujun Hu ◽  
Qingjie Xia ◽  
...  
Keyword(s):  
Electromagnetic Fields ◽  
Signalling Pathways ◽  
Pulsed Electromagnetic Fields ◽  
Pulsed Electromagnetic ◽  
Ovariectomised Rats

CT scan-evaluated outcome of pulsed electromagnetic fields in the treatment of acute scaphoid fractures

2014 ◽  
Vol 96-B (8) ◽  
pp. 1070-1076 ◽  
Author(s):  
P. F. W. Hannemann ◽  
M. R. van Wezenbeek ◽  
K. A. Kolkman ◽  
E. L. L. Twiss ◽  
C. H. J. Berghmans ◽  
...  
Keyword(s):  
Ct Scan ◽  
Electromagnetic Fields ◽  
Pulsed Electromagnetic Fields ◽  
Scaphoid Fractures ◽  
Pulsed Electromagnetic
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