Endocrine disruptors and reproductive health: The case of bisphenol-A

2006 ◽  
Vol 254-255 ◽  
pp. 179-186 ◽  
Author(s):  
Maricel V. Maffini ◽  
Beverly S. Rubin ◽  
Carlos Sonnenschein ◽  
Ana M. Soto
Keyword(s):  
Reproductive Health ◽  
Bisphenol A ◽  
Endocrine Disruptors

Study of Endocrine Disruptors in the Sediment of a South Brazilian River

2015 ◽  
Vol 1120-1121 ◽  
pp. 862-866
Author(s):  
Irajá Do Nascimento ◽  
Nathália Christine Vieceli ◽  
Michele Schmitz ◽  
Fernanda Glaeser
Keyword(s):  
Gas Chromatography ◽  
Bisphenol A ◽  
Endocrine Disruptors ◽  
River Sediment ◽  
Flame Ionization Detection ◽  
River Contamination ◽  
Ionization Detection ◽  
Flame Ionization ◽  
Organic Extracts ◽  
Ethylhexyl Phthalate

This study investigated the occurrence of di (2-ethylhexyl) phthalate (DEHP) di-n-butylphthalate (DBP) and bisphenol A (BPA) in river sediment. The samples were collected from three selected points and extracted by sonication, using n-hexane and ethanol. The organic extracts were analyzed by gas chromatography with flame ionization detection (GC/FID). DBP and BPA were not detected. The average concentrations of DEHP range from 0.72 (±0.04) to 27.90 (± 3.05) ng g-1 of sediment. The best solvent for the extractions was n-hexane. However ethanol also shows good extraction yields of DEHP. These results showed an important anthropic contribution for the river contamination by endocrine disruptors.

scholarly journals Pesticides as endocrine distruptors of the reproductive system (literature review and own research)

2021 ◽  
pp. 49-62
Author(s):  
Ninel Shepelska ◽  
Mykola Prodanchuk ◽  
Yana Kolianchuk
Keyword(s):  
Reproductive Health ◽  
Endocrine Disruptors ◽  
Reproductive System ◽  
Reproductive Toxicity ◽  
Endocrine System ◽  
Reproductive Function ◽  
Dose Dependence ◽  
Animal Cells ◽  
Wide Range ◽  
The Impact

Currently, one of the main threats to human health is undoubtedly endocrine disruptors (ED), since they directly disrupt the processes of homeostasis maintenance, controlled by the endocrine system, the purpose of which is to maintain normal functions and development in a constantly changing environment. Pesticides can disrupt the physiological functioning of many endocrine axes, including the endocrine mechanisms that ensure reproductive health. It should be noted that research aimed at preventing chemically induced reproductive disorders in the human population is one of the central areas of preventive medicine, both in terms of their importance and the complexity of the tasks being solved. Analysis and generalization of the results of our own long-term studies have shown that the selective, and, therefore, the most dangerous toxicity of pesticides for the reproductive system is determined by endocrine-mediated mechanisms of etiopathogenesis. The low level of doses inducing pathological changes in reproductive function in our studies fully confirms one of the universal signs inherent in endocrine-distruptive compounds. The above examples demonstrate a wide range of possible endocrine-mediated mechanisms of reproductive toxicity of pesticides - endocrine disruptors. However, it is very important to note that low doses may be more effective in changing some endpoints compared to high (toxic) doses. Currently, several mechanisms have been identified and studied that demonstrate how hormones and ED induce non-monotonic reactions in animal cells, tissues and organs. The reproductive system, the functioning of which is ensured by a fine balancing of the action of androgens and estrogens, is one of the systems that presents a unique opportunity for modeling a non-monotonic dose dependence. All of the above indicates the extreme danger of the impact of hormonally active agents on the reproductive health of a person and his offspring. At the same time, the threat of endocrine-mediated disorders for subsequent generations can also be realized through the induction of mechanisms of development of epigenetic transgenerational effects. Taking into account the results of studies of the mechanisms of the ED destructive action, as well as their ability to induce non-monotonic dose dependence at an extremely low dose level, it should be admitted that, apparently, there is a need to revise the paradigm of methodological approaches to the regulation of pesticides with endocrine-disruptive properties. Key words: pesticides, endocrine disruptors, reproductive system

P–006 Simultaneous determination of bisphenol A and S in the samples of human seminal fluid

2021 ◽  
Vol 36 (Supplement_1) ◽  
Author(s):  
S Fialková ◽  
T Král ◽  
J Kohoutek ◽  
K Franzová ◽  
M Ješeta ◽  
...  
Keyword(s):  
Sample Preparation ◽  
Bisphenol A ◽  
Endocrine Disruptors ◽  
Analytical Method ◽  
Seminal Fluid ◽  
Limit Of Detection ◽  
Semen Parameters ◽  
Ms Detection ◽  
Validation Criteria ◽  
Limit Of Quantitation

Abstract Study question Can we quantitatively determine concentrations of endocrine disruptors namely bisphenol A and S in seminal fluid? Summary answer We developed selective analytical method to simultaneously screen for the presence of bisphenol A (BPA) and S (BPS). What is known already The male reproductive system involves processes, which may be influenced by the disruption of the endocrine system by chemicals called endocrine disruptors (EDs). There is a growing evidence that EDs such as bisphenol A and S may be responsible for the decline in male reproductive health. To date, the claimed adverse effects on male fertility are largely based on the results from studies assessing the relationship between urinary BPA and BPS concentration and semen parameters. The best evidence of an adverse effect of BPA and BPS directly on spermatozoa could be provided by measuring bisphenols concentration directly in seminal fluid. Study design, size, duration To selectively and quantitatively analyzed bisphenols in any biological matrix advanced analytical tools and selective sample preparation protocols must be employed. In this study we developed targeted analytical method based on liquid chromatography tandem mass (LC-MS/MS) detection to measure bisphenol A and S in seminal fluid samples obtained from IVF clinic. A total of 140 samples were analysed. Participants/materials, setting, methods BPA and BPS was extracted from 140 seminal fluid samples using solvent extraction followed by preconcentration step. Samples were analyzed on Agilent 6495 Triple Quadrupole (Agilent Technologies, Santa Clara, CA) operating in the ESI-negative mode. Two MS/MS transitions were used for quantitative LC-MS/MS analyses. Chromatographic separation was achieved on Waters™ ACQUITY™ UPLC™BEH C18 (100 × 2.1 mm, 1.7 µm) column using gradient elution with a mixture of 0.1mM ammonium fluoride and methanol as mobile phases. Main results and the role of chance We developed selective sample preparation method for detection of BPA and BPS in seminal fluid followed by LC-MS/MS detection. The method validation was performed based on FDA guidelines. Validation criteria included limit of detection (LOD), limit of quantitation (LOQ), accuracy and precision. Due to the lack of the certified reference material the validation criteria of the method were assessed in pool of spiked seminal samples. The accuracy of the LC-MS/MS method was evaluated as a percent recovery of the amount of target analyte added into the sample. Recovery rates were above 80% for both analytes. LOD was 0.04 ng/mL for BPA and 0.01 ng/mL for BPS. LOQ was 0.14 ng/mL and 0.02 ng/mL for BPS. Measured BPA concentration ranged from 0.04 ng/mL to 1.62 ng/mL. For BPS, the concentration ranged from 0.01 ng/mL to 0.47 ng/mL. BPA and BPS were detected in 64% and 81% of samples, respectively. Interestingly, BPA showed lower detection frequency compared to BPS. These results are consistent with other studies performed on urine samples. Limitations, reasons for caution The limitation of the developed method is the time-consuming sample preparation and analysis cost. Wider implications of the findings: These results document for the first time the presence of BPS in seminal fluid. Knowing the concentration of BPA and BPS in seminal fluid is crucial for mitigating the associated health risks and initiating intervention and prevention strategies. Our future work will evaluate the influence of BPS concentration on spermatozoa. Trial registration number AZV NV18–01–00544; CZ.02.2.69/0.0/0.0/19_074/0012727

Biotransformation of genistein and bisphenol A in cell lines used for screening endocrine disruptors

2008 ◽  
Vol 22 (6) ◽  
pp. 1595-1604 ◽  
Author(s):  
J. Bursztyka ◽  
E. Perdu ◽  
K. Pettersson ◽  
I. Pongratz ◽  
M. Fernández-Cabrera ◽  
...  
Keyword(s):  
Bisphenol A ◽  
Endocrine Disruptors ◽  
Cell Lines

scholarly journals DNA oxidation induced by fetal exposure to BPA agonists impairs female meiosis

2020 ◽  
Author(s):  
Sonia Abdallah ◽  
Delphine Moison ◽  
Margaux Wieckowski ◽  
Sébastien Messiaen ◽  
Emmanuelle Martini ◽  
...  
Keyword(s):  
Bisphenol A ◽  
Endocrine Disruptors ◽  
In Utero ◽  
Dna Oxidation ◽  
Common Mechanism ◽  
Fetal Exposure ◽  
Female Meiosis ◽  
Dna Damages ◽  
Germ Cell Differentiation ◽  
Bpa Analogs

SummaryMany endocrine disruptors have been proven to impair the meiotic process that is mandatory to produce healthy gametes. Bisphenol A is emblematic as it impairs meiotic prophase I and causes oocyte aneuploidy following in utero exposure. However, the mechanisms underlying these deleterious effects remain poorly understood. Furthermore, the increasing uses of BPA analogs raise concerns for public health. Here, we investigated the effect on oogenesis in mouse of fetal exposure to two BPA analogs, Bisphenol A Diglycidyl Ether (BADGE) or Bisphenol AF (BPAF). These analogs delay meiosis initiation, increase MLH1 foci per cell and induce oocyte aneuploidy. We further demonstrate that these defects are accompanied by a deregulation of gene expression and aberrant mRNA splicing in fetal premeiotic germ cells. Interestingly, we observed an increase in DNA oxidation after exposure to BPA analogs. Specific induction of oxidative DNA damages during fetal germ cell differentiation causes similar defects during oogenesis, as observed in 8-Oxoguanine DNA Glycosylase (OGG1) deficient mice or after in utero exposure to potassium bromate (KBrO3), an inducer of oxidative DNA damages. Moreover, the supplementation of N-acetylcysteine (NAC) with BPA analogs counteracts the bisphenol-induced meiotic effect. Together our results position oxidative stress as a central event that negatively impacts the female meiosis with major consequences on oocyte quality. This could be a common mechanism of action for so called endocrine disruptors pollutants and it could lead to novel strategies for reprotoxic compounds.

scholarly journals Endocrine disruptive compounds and male reproduction

2018 ◽  
Vol 6 (4) ◽  
pp. 131-134
Author(s):  
Ješeta Michal ◽  
Chmelíková Eva ◽  
Crha Igor ◽  
Sedmíková Markéta ◽  
Žáková Jana ◽  
...  
Keyword(s):  
Reproductive Health ◽  
Endocrine Disruptors ◽  
Hormonal Regulation ◽  
Sperm Quality ◽  
Postnatal Period ◽  
Male Reproduction ◽  
Testicular Development ◽  
Negative Effects ◽  
Hormone Synthesis ◽  
The Impact

AbstractEndocrine disruptors (EDs) are chemical substances that affect physiological processes in the organism via hormonal regulation. The EDs are present in the environment and objects of everyday use. They are often detected in food, particularly released from packaging of canned food, but also from plastic water bottles, and they are also found in cosmetics and fertilizers. They are commonly detected in children's toys, banknotes, receipts and many more objects. Permanent and long-term utilization of EDs has harmful effects on human reproductive health mainly by interference with sex hormone synthesis and mechanism of action. The endocrine disruptors show many negative effects on male reproductive system. Any change during synthesis or activity of sex hormones can cause abnormal reproduction, including developmental anomalies of the sexual system, disruption of testicular development or deterioration of sperm quality. Mainly the impact on the development of testicles in prenatal and early postnatal period can be crucial for reproductive health in males. This review provides an overview of the EDs and their possible impact on reproductive health in males with focus on sperm quality and development of testicles.

scholarly journals ENDOCRINE DISRUPTORS: BISPHENOL A AND ITS RELATION WITH OBESITY

2018 ◽  
Vol 2 (4) ◽  
pp. 41-49 ◽  
Author(s):  
Lucas Wanderley Rodrigues Gonçalves de S Sá ◽  
Luciano Nunes de Sousa ◽  
Thyciara Fontenele Marques ◽  
Gabriel Pereira Bernardo ◽  
Lorena Pereira Bernardo ◽  
...  
Keyword(s):  
Bisphenol A ◽  
Endocrine Disruptors ◽  
Endocrine System ◽  
Signaling Mechanism ◽  
High Exposure ◽  
Contemporary World ◽  
The Metabolic Syndrome ◽  
Endocrine Disrupting ◽  
Prevalence Of Obesity ◽  
Current Context

The prevalence of obesity is, worldwide, in wide intensification. This increase stimulates the search for new hypotheses that explain the genesis of the metabolic syndrome. This article seeks to clarify the obesogenic hypothesis of bisphenol A (BPA), based on its endocrine disrupting potential. Methodology: For the development of the present study, the Scopus and PubMed databases were used, from descriptors generated by the DeCS: Endocrine Disruptors; Foods; Obesity; Bisphenol A and its correspondents in English. Randomized and controlled trials were considered eligible, focusing on publications in English and Portuguese. The survey was conducted on April 11, 2017, with publications of the last five years being leaked. Based on this, 87 articles were obtained and, from the reading of the title and its abstracts, 26 publications were selected. After the complete reading of the 26 articles selected, 18 articles were obtained that served as a basis for this bibliographic review. Bisphenol A appears as an important agent that causes obesity in the contemporary world, interfering in the signaling mechanism of the endocrine system, being its high exposure linked to many of the habits present in the current context. Conclusion: Based on the possible consequences of bisphenol A, the reduction of human exposure to the compound should be considered. Based on this, alternatives to BPA in the production of industrial plastic polymers have to be considered since increased exposure to the endocrine disruptor is closely related to industrial production. Keywords: Endocrine Disruptors, Obesity, Bisphenol A

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