Design and development of a low-cost open-source 3D printer and its single response optimization using polylactic acid (PLA) material

2020 ◽  
Vol 27 ◽  
pp. 2981-2991
Author(s):  
Rishabh Sood ◽  
Sharad K. Pradhan
Author(s):  
A. Elibiary ◽  
W. Oakey ◽  
S. Jun ◽  
B. Sanz-Izquierdo ◽  
D. Bird ◽  
...  

2021 ◽  
Author(s):  
Jiang Xu ◽  
Zhuowei Du ◽  
Paul Hsi Liu ◽  
Yi Kou ◽  
Lin Chen

We introduce OPAM, an Open source, low-cost (under $150), 3D-Printed, stepper motor driven, Arduino based, single cell Micromanipulator (OPAM). Modification of a commercial stepper motor led to dramatically increased stability and maneuverability of the motor, based on which the micromanipulator was designed. All components of this micromanipulator can be 3D printed using an entry-level 3D printer and assembled with ease. With this single cell manipulator, successful targeted single cell capture and transfer was confirmed under the microscope, which showed great promise for single cell related experiments.


PLoS ONE ◽  
2021 ◽  
Vol 16 (6) ◽  
pp. e0251812
Author(s):  
Arunkumar Arumugam ◽  
Cole Markham ◽  
Saurabh S. Aykar ◽  
Barbara Van Der Pol ◽  
Paula Dixon ◽  
...  

Growth in open-source hardware designs combined with the decreasing cost of high-quality 3D printers have supported a resurgence of in-house custom lab equipment development. Herein, we describe a low-cost (< $400), open-source CO2 incubator. The system is comprised of a Raspberry Pi computer connected to a 3D printer controller board that has controls for a CO2 sensor, solenoid valve, heater, and thermistors. CO2 is supplied through the sublimation of dry ice stored inside a thermos to create a sustained 5% CO2 supply. The unit is controlled via G-Code commands sent by the Raspberry Pi to the controller board. In addition, we built a custom software application for remote control and used the open-source Grafana dashboard for remote monitoring. Our data show that we can maintain consistent CO2 and temperature levels for over three days without manual interruption. The results from our culture plates and real-time PCR indicate that our incubator performed equally well when compared to a much more expensive commercial CO2 incubator. We have also demonstrated that the antibiotic susceptibility assay can be performed in this low-cost CO2 incubator. Our work also indicates that the system can be connected to incubator chambers of various chamber volumes.


Author(s):  
Matthew Lanaro ◽  
Jacob Skewes ◽  
Logan Spiers ◽  
Prasad K. Yarlagadda ◽  
Maria A. Woodruff

2021 ◽  
Vol 11 (7) ◽  
pp. 3197
Author(s):  
Jose Luis Saorin ◽  
Manuel Drago Diaz-Alemán ◽  
Jorge De la Torre-Cantero ◽  
Cecile Meier ◽  
Ithaisa Pérez Conesa

The adoption of open-source digital manufacturing technologies in small art workshops may improve their competitiveness. Pieces modeled by computer and made with FDM (Fused Deposition Modeling) 3D printers that use PLA (polylactic acid) can be implemented in the procedures of artistic casting. However, models printed by PLA are limited to approximate minimum sizes of 3 cm, and the optimal layer height resolution is 0.1 mm. These sizes and resolutions are not suitable for creating microsculptures used, in many cases, in jewelry. An alternative to solve this limitation, is to use a DMLS (Direct Metal Laser Sintering) 3D printer. However, due to its high cost, it is a technology that is difficult to introduce in small artistic foundries. This work detailed the design and validation of a DLP (Digital Light Processing) 3D printer, using backlit LCD (Liquid Crystal Display) screens with ultraviolet light. Its development is totally “open source” and is proposed as a kit made up of electronic components, based on Arduino and easy to access mechanical components in the market. Most parts can be manufactured in low cost FDM (Fused Deposition Modeling) 3D printers. The result is an affordable, high resolution (0.021 mm), and open-design printer that can be implemented in artistic contexts.


2018 ◽  
Vol 6 (1) ◽  
pp. e1646 ◽  
Author(s):  
Marco A. Rendón-Medina ◽  
Laura Andrade-Delgado ◽  
Jose E. Telich-Tarriba ◽  
Antonio Fuente-del-Campo ◽  
Carlos A. Altamirano-Arcos

2018 ◽  
Vol 15 (2) ◽  
pp. 666-675
Author(s):  
Nor Aiman Sukindar ◽  
Mohd Khairol Anuar Mohd Ariffin ◽  
B. T. Hang Tuah Baharudin ◽  
Che Nor Aiza Jaafar ◽  
Mohd Idris Shah Ismail

Additive manufacturing, also known as three-dimensional (3D) printing, is the process of developing 3D products in a layer-by-layer manner using filament as a material feedstock to create a solid structure. Owing to its unique properties and advantages, which include biodegradability and printing speed, polylactic acid is one of the most common 3D printing extrusion materials. While a considerable attention has been paid to the manipulation of process parameters in order to achieve desired finished product quality, to date less research has been performed on improving the hardware systems of low-cost 3D printers. This study focuses on fabricating the 3D printer nozzle parts, with an emphasis on die angle, nozzle diameter, liquefier design, and insulator composition. Modifying the properties of these components from the conventional nozzle, it is possible to optimize the stability and accuracy of the extrusion process, leading to better-quality printed products. To demonstrate the capability of the new nozzle, its tensile and compressive strengths were compared to those of a conventional nozzle. The obtained results proved that the proposed augmentations to the nozzle system lead to finished products with improved mechanical properties.


2020 ◽  
Author(s):  
George O. T. Merces ◽  
Conor Kennedy ◽  
Blanca Lenoci ◽  
Emmanuel G. Reynaud ◽  
Niamh Burke ◽  
...  

AbstractCommercial live cell imaging systems represent a large financial burden to research groups, while current open source incubator microscopy systems lack adaptability and are sometimes inadequate for complex imaging experimentation. We present here a low-cost microscope designed for inclusion within a conventional tissue culture incubator. The build is constructed using an entry level 3D printer as the basis for the motion control system, with Raspberry Pi imaging and software integration, allowing for reflected, oblique, and fluorescence imaging of live cell monolayers. The open source nature of the design is aimed to facilitate adaptation by both the community at large and by individual researchers/groups. The development of an adaptable and easy-to-use graphic user interface (GUI) allows for the scientist to be at the core of experimental design. Simple modifications of the base GUI code, or generation of an entirely purpose-built script, will allow microscopists to place their experimental design as the priority, as opposed to designing experiments to fit their current equipment. The build can be constructed for a cost of roughly C1000 and thus serves as a low-cost and adaptable addition to the open source microscopy community.Abstract Figure


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