Effects of anthropogenic seawater acidification on acid–base balance in the sea urchin Psammechinus miliaris

2007 ◽  
Vol 54 (1) ◽  
pp. 89-96 ◽  
Author(s):  
Hayley Miles ◽  
Stephen Widdicombe ◽  
John I. Spicer ◽  
Jason Hall-Spencer
Keyword(s):  
Sea Urchin ◽  
Acid Base Balance ◽  
Acid Base ◽  
Seawater Acidification ◽  
Psammechinus Miliaris ◽  
Base Balance

scholarly journals Intracellular pH Regulation: Characterization and Functional Investigation of H+ Transporters in Stylophora Pistillata

2020 ◽  
Author(s):  
Laura Capasso ◽  
Philippe Ganot ◽  
Víctor Planas-Bielsa ◽  
Sylvie Tambutté ◽  
Didier Zoccola
Keyword(s):  
Intracellular Ph ◽  
Ph Regulation ◽  
Genomic Structure ◽  
Expression Patterns ◽  
Acid Base Balance ◽  
Acid Base ◽  
Specific Expression ◽  
Stylophora Pistillata ◽  
Seawater Acidification ◽  
Base Balance

Abstract Background: Reef-building corals regularly experience changes in intra and extracellular H+ concentration ([H+]) due to physiological and environmental processes. Stringent control of [H+] is required for the maintenance of homeostatic acid-base balance in coral cells and is achieved through the regulation of intracellular pH (pHi). This task is especially challenging for reef-building corals that share an endosymbiotic relationship with photosynthetic dinoflagellates (family Symbiodinaceae), which exert a significant effect on the pHi of coral cells. Despite their importance, the pH regulatory proteins involved in the homeostatic acid-base balance have been scarcely investigated in corals. Here, we reported the full characterisation in terms of genomic structure, domain topology and phylogeny of three majors H+ transporter families implicated in pHi regulation; we investigated their tissue-specific expression and we assessed the effect of seawater acidification on their level of expression.Results: We identified members of the Na+/H+ exchanger (SLC9), vacuolar-type electrogenic H+-ATP hydrolases (V-ATPase) and voltage-gated proton channels (HvCN) families in the genome and transcriptome of S. pistillata. In addition, we identified a novel member of the HvCN gene family in the cnidarian subclass Hexacorallia, which has never been described in any species to date. We also reported key residues that participate to the H+ transporters substrate specificity, protein function and regulation. Lastly, we demonstrated that some of these have different tissue expression patterns and are mostly unaffected by exposure to seawater acidification.Conclusions: In this study, we provide the first characterization of the H+ transporters genes that contribute to homeostatic acid-base balance in coral cells. This work will enrich knowledge about basic aspects of coral biology, bearing important implications for our understanding of how corals regulate their intracellular environment.

scholarly journals Intracellular pH regulation: characterization and functional investigation of H+ transporters in Stylophora pistillata

2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Laura Capasso ◽  
Philippe Ganot ◽  
Víctor Planas-Bielsa ◽  
Sylvie Tambutté ◽  
Didier Zoccola
Keyword(s):  
Intracellular Ph ◽  
Ph Regulation ◽  
Expression Patterns ◽  
Acid Base Balance ◽  
Acid Base ◽  
Stylophora Pistillata ◽  
Intracellular Ph Regulation ◽  
Seawater Acidification ◽  
Base Balance

AbstractBackgroundReef-building corals regularly experience changes in intra- and extracellular H+concentrations ([H+]) due to physiological and environmental processes. Stringent control of [H+] is required to maintain the homeostatic acid-base balance in coral cells and is achieved through the regulation of intracellular pH (pHi). This task is especially challenging for reef-building corals that share an endosymbiotic relationship with photosynthetic dinoflagellates (family Symbiodinaceae), which significantly affect the pHiof coral cells. Despite their importance, the pH regulatory proteins involved in the homeostatic acid-base balance have been scarcely investigated in corals. Here, we report in the coralStylophora pistillataa full characterization of the genomic structure, domain topology and phylogeny of three major H+transporter families that are known to play a role in the intracellular pH regulation of animal cells; we investigated their tissue-specific expression patterns and assessed the effect of seawater acidification on their expression levels.ResultsWe identified members of the Na+/H+exchanger (SLC9), vacuolar-type electrogenic H+-ATP hydrolase (V-ATPase) and voltage-gated proton channel (HvCN) families in the genome and transcriptome ofS. pistillata. In addition, we identified a novel member of the HvCN gene family in the cnidarian subclass Hexacorallia that has not been previously described in any species. We also identified key residues that contribute to H+transporter substrate specificity, protein function and regulation. Last, we demonstrated that some of these proteins have different tissue expression patterns, and most are unaffected by exposure to seawater acidification.ConclusionsIn this study, we provide the first characterization of H+transporters that might contribute to the homeostatic acid-base balance in coral cells. This work will enrich the knowledge of the basic aspects of coral biology and has important implications for our understanding of how corals regulate their intracellular environment.

Higher Dietary Acidity is Associated with Lower Bone Mineral Density in Postmenopausal Iranian Women, Independent of Dietary Calcium Intake

2014 ◽  
Vol 84 (3-4) ◽  
pp. 0206-0217 ◽  
Author(s):  
Seyedeh-Elaheh Shariati-Bafghi ◽  
Elaheh Nosrat-Mirshekarlou ◽  
Mohsen Karamati ◽  
Bahram Rashidkhani
Keyword(s):  
Calcium Intake ◽  
Dietary Calcium ◽  
Dietary Calcium Intake ◽  
Dietary Intakes ◽  
Iranian Women ◽  
Acid Base Balance ◽  
Mineral Density ◽  
Acid Base ◽  
Base Balance ◽  
Dietary Acid

Findings of studies on the link between dietary acid-base balance and bone mass are relatively mixed. We examined the association between dietary acid-base balance and bone mineral density (BMD) in a sample of Iranian women, hypothesizing that a higher dietary acidity would be inversely associated with BMD, even when dietary calcium intake is adequate. In this cross-sectional study, lumbar spine and femoral neck BMDs of 151 postmenopausal women aged 50 - 85 years were measured using dual-energy x-ray absorptiometry. Dietary intakes were assessed using a validated food frequency questionnaire. Renal net acid excretion (RNAE), an estimate of acid-base balance, was then calculated indirectly from the diet using the formulae of Remer (based on dietary intakes of protein, phosphorus, potassium, and magnesium; RNAERemer) and Frassetto (based on dietary intakes of protein and potassium; RNAEFrassetto), and was energy adjusted by the residual method. After adjusting for potential confounders, multivariable adjusted means of the lumbar spine BMD of women in the highest tertiles of RNAERemer and RNAEFrassetto were significantly lower than those in the lowest tertiles (for RNAERemer: mean difference -0.084 g/cm2; P=0.007 and for RNAEFrassetto: mean difference - 0.088 g/cm2; P=0.004). Similar results were observed in a subgroup analysis of subjects with dietary calcium intake of >800 mg/day. In conclusion, a higher RNAE (i. e. more dietary acidity), which is associated with greater intake of acid-generating foods and lower intake of alkali-generating foods, may be involved in deteriorating the bone health of postmenopausal Iranian women, even in the context of adequate dietary calcium intake.

The Effect of Long-term Topiramate Treatment on Acid-base Balance in Children with Epilepsy

2016 ◽  
Vol 24 (3) ◽  
pp. 116-121
Author(s):  
김지용 ◽  
남상욱 ◽  
김영미 ◽  
이윤진 ◽  
이훈상 ◽  
...  
Keyword(s):  
Acid Base Balance ◽  
Acid Base ◽  
Base Balance
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