Effects of aerobic exercise training on antioxidant enzyme activities and mRNA levels in soleus muscle from young and aged rats

2007 ◽  
Vol 128 (3) ◽  
pp. 267-275 ◽  
Author(s):  
Rafael H. Lambertucci ◽  
Adriana Cristina Levada-Pires ◽  
Luciana V. Rossoni ◽  
Rui Curi ◽  
Tania C. Pithon-Curi
Keyword(s):  
Exercise Training ◽  
Aerobic Exercise ◽  
Antioxidant Enzyme ◽  
Soleus Muscle ◽  
Enzyme Activities ◽  
Aerobic Exercise Training ◽  
Antioxidant Enzyme Activities ◽  
Mrna Levels ◽  
Aged Rats

Exercise modulates antioxidant enzyme gene expression in rat myocardium and liver

2000 ◽  
Vol 88 (5) ◽  
pp. 1791-1796 ◽  
Author(s):  
D. O. Wilson ◽  
P. Johnson
Keyword(s):  
Glutathione Peroxidase ◽  
Antioxidant Enzyme ◽  
Enzyme Activities ◽  
Transcriptional Control ◽  
Rnase Protection Assay ◽  
Antioxidant Enzyme Activities ◽  
Mrna Levels ◽  
Rnase Protection ◽  
Spontaneously Hypertensive ◽  
Wistar Kyoto

Our previous studies have shown that exercise caused changes in the tissue activities of the antioxidant enzymes glutathione peroxidase, superoxide dismutase, and catalase in spontaneously hypertensive (SH) and Wistar-Kyoto (WKY) rats. To determine whether the changes observed were due to changes in mRNA levels of the enzymes, levels of tissue mRNA were determined by quantitative RNase protection assay. Comparisons of tissue enzyme activities and mRNA levels in sedentary and exercised animals showed that, in some cases (e.g., glutathione peroxidase in SH and WKY myocardium), parallel changes in enzyme activity and mRNA levels occurred, whereas in other cases (e.g., catalase in SH and WKY liver), nonparallel changes were found. Exercise of hypertensive rats altered antioxidant enzyme mRNA levels to those seen in normotensive animals in some, but not all, cases. The results suggest that transcriptional control over changes in exercise-related antioxidant enzyme activities is operative in some cases, although in other cases posttranscriptional regulatory mechanisms may exist.

Aging and exercise training in skeletal muscle: responses of glutathione and antioxidant enzyme systems

1994 ◽  
Vol 267 (2) ◽  
pp. R439-R445 ◽  
Author(s):  
C. Leeuwenburgh ◽  
R. Fiebig ◽  
R. Chandwaney ◽  
L. L. Ji
Keyword(s):  
Skeletal Muscle ◽  
Exercise Training ◽  
Antioxidant Enzyme ◽  
Enzyme Activities ◽  
Vastus Lateralis ◽  
Antioxidant Systems ◽  
Antioxidant Enzyme Activities ◽  
Vastus Lateralis Muscle ◽  
Adult Rats ◽  
Young Rats

Glutathione (GSH) content and antioxidant enzyme activities were investigated in skeletal muscle of young, adult, and old male Fischer 344 rats. Furthermore, the effect of 10 wk of exercise training on these antioxidant systems was evaluated at all ages. In the soleus muscle, GSH concentration increased markedly with age, with no significant change in glutathione disulfide (GSSG) content. Training caused a 30% decrease of GSH (P < 0.05) in the soleus of young rats and a reduction of the GSH-to-GSSG ratio at all ages. Activity of gamma-glutamyl transpeptidase (GGT), a key enzyme for GSH uptake by muscle, was also significantly decreased with training. GSH, GSSG, and the GSH-to-GSSG ratio were not altered with aging or training in the deep portion of vastus lateralis muscle (DVL). Activities of GSH peroxidase (GPX), GSSG reductase (GR), superoxide dismutase (SOD), catalase (CAT), and GSH sulfur-transferase were increased significantly with aging in both soleus and DVL. In DVL, training increased GPX and SOD activities in the young rats, whereas in soleus, training decreased GR and CAT activities in the adult rats and GGT and CAT activities in the old rats. Muscle lipid peroxidation was significantly increased with aging in both DVL and soleus but was not affected by training. These data indicate that aging may cause not only an overall elevation of antioxidant enzyme activities but also a fiber-specific adaptation of GSH system in skeletal muscle. Exercise training, although increasing selective antioxidant enzymes in the young rats, does not offer additional protection against oxidative stress in the senescent muscle.

Age-Related Attenuation of Aerobic Exercise Training Adaptation in 24-Week Old Rats

Diabetes ◽  
2018 ◽  
Vol 67 (Supplement 1) ◽  
pp. 1916-P
Author(s):  
REBECCA L. SCALZO ◽  
GRAHAME F. EVANS ◽  
SARA E. HULL ◽  
LESLIE KNAUB ◽  
LORI A. WALKER ◽  
...  
Keyword(s):  
Exercise Training ◽  
Aerobic Exercise ◽  
Aerobic Exercise Training ◽  
Training Adaptation ◽  
Age Related ◽  
Old Rats
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