Quickly verifying the antioxidant contribution of the individual composition in natural antioxidants by HPLC-free radical scavenging detection

LWT ◽  
2018 ◽  
Vol 96 ◽  
pp. 461-468 ◽  
Author(s):  
Lu Wang ◽  
You Luo ◽  
Yanan Wu ◽  
Fenggeng Xia ◽  
Zhenqiang Wu
Keyword(s):  
Free Radical ◽  
Radical Scavenging ◽  
Natural Antioxidants ◽  
Free Radical Scavenging ◽  
The Individual ◽  
Individual Composition

scholarly journals Fractionation, Phytochemical Screening and Free Radical Scavenging Capacity of Different Sub-Fractions from Pituranthos scoparius Roots

2020 ◽  
Vol 10 (3) ◽  
pp. 133-136
Author(s):  
Ahlem Karbab ◽  
Kamel Mokhnache ◽  
Lekhmici Arrar ◽  
Abderahmane Baghiani ◽  
Seddik Khennouf ◽  
...  
Keyword(s):  
Free Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Health Benefit ◽  
Natural Antioxidants ◽  
Chloroform Extract ◽  
Free Radical Scavenging ◽  
Scavenging Activity ◽  
Phytochemical Screening ◽  
Potential Health

The purpose of this study was to prepare three sub-fractions from Pituranthos scoparius roots (PSR), characterize their phytochemicals contents and to investigate their free radical scavenging activity by 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and hydroxyl scavenging activities. Tannins, flavonoids, steroids, and other bioactive compounds were found in the different sub-fractions. The Ethyle acetate extract (EAE) and chloroform extract (ChE) exhibited the highest antioxidant activity using ABTS (17.8 ± 0.87 µg/mL and 18.15 ± 0.68 µg/mL), respectively. Whereas, Crude extract (CrE) have been presented strong hydroxyl scavenging activity (14.9 ± 0.8 µg/mL). This study indicates that PSR extracts has potent free radical scavenging, and may prove to be of potential health benefit as well as additional resources for natural antioxidants. Keywords: Medicinal plant; phytochemical screening; sub-fractions, free radical scavenging.

scholarly journals Preliminary Phytochemical Screening and In vitro Antioxidant Activity of Ethiopian Indigenous Medicinal Plants, Ocimum lamiifolium Hochst. ex Benth and Ocimum basilicum L.

2016 ◽  
Vol 8 (01) ◽  
Author(s):  
Suresh Kumar P. Nair ◽  
Kumar Ganesan ◽  
Henok Azalewor ◽  
Neethu Letha ◽  
Sharmila Gani
Keyword(s):  
Free Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Natural Antioxidants ◽  
Free Radical Scavenging ◽  
Ocimum Basilicum ◽  
Scavenging Activity ◽  
Phytochemical Screening ◽  
Phytochemical Investigation

Antioxidants are the chemical substances which prevent the free radicals damage in the body. Numerous researches are going globally focussed on investigating natural antioxidants of plants origins. The aims of the present study were to evaluate preliminary phytochemical investigation and in vitro antioxidant activities of Ethiopian indigenous medicinal plants, Ocimum lamiifolium Hochst. ex Benth and Ocimum basilicum L. Aqueous, benzene and hexane crude leaves extracts of O. lamiifolium and O. basilicum were subjected to qualitative phytochemical screening using standard procedures. In addition, we investigated the antioxidant potential of crude aqueous leaves extract of O. lamiifolium and O. basilicum using tests involving inhibition of superoxide anions, DPPH, H2O2, NO and ABTS. Preliminary phytochemical investigation for benzene, hexane and aqueous extracts found alkaloids, sterols, carbohydrate and glycosides, tannins and flavonoids. The fraction inhibition of lipid peroxide at the first stage of oxidation illustrated antioxidant activity of O. lamiifolium and O. basilicum as 90% and 88% compared to those of gallic acid (97%) and BHT (84%) respectively. Also, the aqueous leaves extract of O. lamiifolium and O. basilicum exhibited significant DPPH free radical scavenging activity, nitric acid free radical scavenging activity assay, superoxide anion scavenging activity, ABTS scavenging activity and hydrogen peroxide free radical scavenging assay. Our findings provide confirmation that the aqueous leaves extract of O. lamiifolium and O. basilicum are potential source of natural antioxidants, and this warranted its uses in traditional medicine systems.

Free-radical scavenging actions of natural antioxidants

1998 ◽  
Vol 18 (9) ◽  
pp. 1545-1557 ◽  
Author(s):  
Alejandro D. Boveris ◽  
Susana Puntarulo
Keyword(s):  
Free Radical ◽  
Radical Scavenging ◽  
Natural Antioxidants ◽  
Free Radical Scavenging

Antioxidant and Free Radical Scavenging Activities of N-Modified Chitosans

2014 ◽  
Vol 1002 ◽  
pp. 91-98
Author(s):  
Qing Xu ◽  
Tian Zhong ◽  
Hui Li Li
Keyword(s):  
Free Radical ◽  
Nitrogen Atom ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Natural Antioxidants ◽  
Antioxidant Property ◽  
Free Radical Scavenging ◽  
Biomedical Science ◽  
Modified Chitosan ◽  
Free Radical Scavenging Activities

Recently, the search for effective natural antioxidants use in food and medicinal fields to replace synthetic ones has attracted the most attention. Chitosan, a natural nontoxic biopolymer, is known to possess antioxidant property, which is attributed that NH2 and OH attached in the pyranose ring react with unstable free radicals to form stable macromolecule radicals. It has been observed that the antioxidant activity of chitosan is closely related to the form of nitrogen atom in the molecules. This review focuses on the antioxidant and free radical scavenging activities of various nitrogen atom of chitosan, including N-acylated chitosans, Schiff bases of chitosan, N-alkyl chitosan, and N-quaternized chitosan. The free radical scavenging mechanisms are summarized and the effects on free radical scavenging activity of N-modified chitosan are discussed. This work may provide more insights into the antioxidant ability of N-modified chitosans and potentially enable them to be used as natural antioxidants for application in food, agriculture, cosmetic and biomedical science. Keywords: N-modified chitosan, Sythesis, Antioxidant, Free radical scavenging

Thin-Layer Densitometry as an Alternative Tool in the Quantitative Evaluation of the Free Radical Scavenging Activity of Natural Antioxidants

2005 ◽  
Vol 60 (11) ◽  
pp. 1212-1218 ◽  
Author(s):  
Ehab A. Abourashed
Keyword(s):  
Free Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Natural Antioxidants ◽  
Free Radical Scavenging ◽  
Response Curves ◽  
Chromatographic Separations ◽  
Pure Compounds ◽  
Sample Application ◽  
Free Radical Scavenging Assay

An HPTLC system commonly used for chromatographic separations and analytical quality control has been utilized non-chromatographically as a screening tool for antioxidant activity in the 2,2- diphenyl-1-picrylhydrazyl (DPPH) free-radical scavenging assay. The accurate and precise capabilities for sample application and densitometric analysis of dry spots were applied to determine the 50% inhibition of DPPH (IC50) by a number of natural antioxidants at 530 nm. Concentration-response curves were generated from 3rd degree polynomial fitting of data acquired for each antioxidant at 4 concentration levels. The IC50 values ranged from 9.0 - 332.8 μM with quercetin being the most active and silybin the least active of the pure compounds. Three antioxidant extracts were also evaluated and grapeseed extract was the most active (IC50 8.3 μg/ml). The coefficients of variation for all IC50 values were around 5% which indicated method reproducibility and the suitability of the used system for the intended purpose. Based on its densitometric evaluation of dry spots, the described technique provides an alternative to the spectrophotometric evaluation of samples in solution.

scholarly journals Comparison of Antioxidant Activities of Different Grape Varieties

Molecules ◽  
2018 ◽  
Vol 23 (10) ◽  
pp. 2432 ◽  
Author(s):  
Qing Liu ◽  
Guo-Yi Tang ◽  
Cai-Ning Zhao ◽  
Xiao-Ling Feng ◽  
Xiao-Yu Xu ◽  
...  
Keyword(s):  
Free Radical ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Natural Antioxidants ◽  
Free Radical Scavenging ◽  
Total Phenolic ◽  
Phenolic Contents ◽  
Trolox Equivalent ◽  
Red Grape ◽  
Grape Varieties

Grapes are widely consumed in the world, and different grape varieties could exhibit distinctly different antioxidant activities. In this study, the free radical-scavenging and antioxidant activities of lipophilic, hydrophilic, and insoluble-bound fractions from 30 grape varieties were evaluated by ferric-reducing antioxidant powers (FRAP), Trolox equivalent antioxidant capacities (TEAC), total phenolic contents (TPC), and total flavonoid contents (TFC). The results indicated that the 30 grape varieties exhibited diverse FRAP values (1.289–11.767 μmol Fe(II)/g FW), TEAC values (0.339–4.839 μmol Trolox/g FW), TPC values (0.294–1.407 mg GAE/g FW) and TFC values (0.082–0.132 mg QE/g FW). Several grapes, such as Pearl Black Grape (Xinjiang), Summer Black Grape (Shaanxi), Pearl Green Grape (Xinjiang), Seedless Green Grape (Xinjiang), and Seedless Red Grape (Yunnan), exhibited strong free radical-scavenging and antioxidant activities, which could be consumed as good sources of natural antioxidants to prevent several diseases induced by oxidative stress, such as cardiovascular disease and cancer. Furthermore, several antioxidants were identified and quantified, including caffeic acid, catechin gallate, epicatechin, gallic acid, protocatechuic acid and rutin, which could contribute to the antioxidant activities of grapes.

scholarly journals Enhanced antioxidant activity of Chenopodium formosanum Koidz. by lactic acid bacteria: Optimization of fermentation conditions

PLoS ONE ◽  
2021 ◽  
Vol 16 (5) ◽  
pp. e0249250
Author(s):  
Hsing-Chun Kuo ◽  
Ho Ki Kwong ◽  
Hung-Yueh Chen ◽  
Hsien-Yi Hsu ◽  
Shu-Han Yu ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Lactic Acid ◽  
Free Radical ◽  
Phenolic Compounds ◽  
Lactic Acid Bacteria ◽  
Radical Scavenging ◽  
Natural Antioxidants ◽  
Free Radical Scavenging ◽  
Radical Scavenging Ability ◽  
Optimization Of Fermentation

In this study, different probiotics commonly used to produce fermented dairy products were inoculated independently for Chenopodium formosanum Koidz. fermentation. The strain with the highest level of antioxidant activity was selected and the fermentation process was further optimized via response surface methodology (RSM). Lactobacillus plantarum BCRC 11697 was chosen because, compared to other lactic acid bacteria, it exhibits increased free radical scavenging ability and can produce more phenolic compounds, DPPH (from 72.6% to 93.2%), and ABTS (from 64.2% to 76.9%). Using RSM, we further optimize the fermentation protocol of BCRC 11697 by adjusting the initial fermentation pH, agitation speed, and temperature to reach the highest level of antioxidant activity (73.5% of DPPH and 93.8% of ABTS). The optimal protocol (pH 5.55, 104 rpm, and 24.4°C) resulted in a significant increase in the amount of phenolic compounds as well as the DPPH and ABTS free radical scavenging ability of BCRC 11697 products. The IC50 of the DPPH and ABTS free radical scavenging ability were 0.33 and 2.35 mg/mL, respectively, and both protease and tannase activity increased after RSM. An increase in lower molecular weight (<24 kDa) protein hydrolysates was also observed. Results indicated that djulis fermented by L. plantarum can be a powerful source of natural antioxidants for preventing free radical-initiated diseases.

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