Pigment identification, nutritional composition, bioactivity, and in vitro cancer cell cytotoxicity of Rivina humilis L. berries, potential source of betalains

LWT ◽  
2012 ◽  
Vol 47 (2) ◽  
pp. 315-323 ◽  
Author(s):  
Mohammad Imtiyaj Khan ◽  
P.S.C. Sri Harsha ◽  
P. Giridhar ◽  
G.A. Ravishankar
Keyword(s):  
Cancer Cell ◽  
Nutritional Composition ◽  
Cell Cytotoxicity ◽  
Pigment Identification ◽  
Potential Source

scholarly journals In-vitro cancer cell cytotoxicity and alpha amylase inhibition effect of seven tropical fruit residues

2014 ◽  
Vol 4 ◽  
pp. S665-S671 ◽  
Author(s):  
Priti Gupta ◽  
Ira Bhatnagar ◽  
Se-Kwon Kim ◽  
Ajay Kumar Verma ◽  
Anubhuti Sharma
Keyword(s):  
Cancer Cell ◽  
Alpha Amylase ◽  
Cell Cytotoxicity ◽  
Tropical Fruit ◽  
Inhibition Effect

Glypican-1 as a novel immunotherapeutic target in prostate cancer.

2018 ◽  
Vol 36 (6_suppl) ◽  
pp. 174-174 ◽  
Author(s):  
Alexander Zaslavsky ◽  
Mackenzie Adams ◽  
Sandra Wissmueller ◽  
Douglas Campbell ◽  
Hans Klingemann ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Flow Cytometry ◽  
Cell Lines ◽  
Cancer Cell ◽  
Prostate Cancer Cell ◽  
Cancer Cell Lines ◽  
Parental Line ◽  
Cell Cytotoxicity ◽  
Prostate Cancer Cell Lines

174 Background: New effective therapies for men with prostate cancer are desperately needed. Recently, cancer immunotherapy has emerged as an important new treatment strategy for prostate cancer and for castrate resistant prostate cancer (CRPC). Multiple studies have identified the heparan sulfate proteoglycan-1 Glypican 1 (GPC-1) as being overexpressed in different cancers, and also as being a possible marker of poor prognosis in several solid tumor cancers. GPC-1 has been recently identified as a potential marker for prostate cancer. The MIL-38 monoclonal antibody detects GPC-1 and an IgG1 chimeric version of this antibody has been developed for preclinical studies. Here we sought to examine MIL-38 binding to a panel of prostate cancer cell lines and examine its feasibility as a novel immunotherapeutic agent targeting GPC-1 in prostate cancer Methods: Expression of GPC-1 in CRPC cell lines was examined by Flow cytometry and Western Blotting using MIL-38 as the detector antibody. The competency of GPC-1 as an immunotherapeutic target was assessed via chimeric MIL-38 induced Antibody Dependent Cell Cytotoxicity (ADCC) using high affinity Natural Killer cells (haNKs) in vitro . Results: Flow cytometry and Western blot assessments of normal prostatic epithelial cells (i.e. RWPE-1) and cells from prostate cancer cell lines (i.e. PC-3, 22RV1, DU-145, VCaP, LNCaP, CWR-R1, and LAPC-4) revealed that only cancer cells expressed GPC-1. Enzalutamide resistant cell lines demonstrated higher expression of GPC-1 than their respective parental line. ADCC assays demonstrated enhanced haNK – prostate cancer cell cytotoxicity in the presence of chimeric MIL-38 anti-GPC-1 antibody, while the IgG1 isotype control had no effect. Conclusions: GPC-1 protein was expressed by most prostate cancer cell lines, including enhanced expression by enzalutamide resistant cells. Preliminary in vitro ADCC assay results revealed the potential utility of GPC-1 as an immunotherapeutic target in prostate cancer.

scholarly journals In vitro single-strand DNA damage and cancer cell cytotoxicity effects of Temozolomide

Oncomedicine ◽  
2017 ◽  
Vol 2 ◽  
pp. 102-110 ◽  
Author(s):  
Shruti Purohit ◽  
Devashree Jahagirdar ◽  
Azad Kumar ◽  
Nilesh Kumar Sharma
Keyword(s):  
Dna Damage ◽  
Cancer Cell ◽  
Single Strand ◽  
Cell Cytotoxicity ◽  
Cytotoxicity Effects

794: Valproic Acid Inhibits Prostate Cancer Cell Growth in Vitro and in Vivo

2006 ◽  
Vol 175 (4S) ◽  
pp. 257-257
Author(s):  
Jennifer Sung ◽  
Qinghua Xia ◽  
Wasim Chowdhury ◽  
Shabana Shabbeer ◽  
Michael Carducci ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Valproic Acid ◽  
Cell Growth ◽  
Cancer Cell ◽  
Prostate Cancer Cell ◽  
Cancer Cell Growth

N-hexane Extract and Fraction of Green Tea as Antiproliferation of MCM-B2 Breast Cancer Cells In Vitro

2020 ◽  
Vol 6 (2) ◽  
Author(s):  
Lisni Noraida Waruwu ◽  
Maria Bintang ◽  
Bambang Pontjo Priosoeryanto
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Green Tea ◽  
Cancer Cell ◽  
Breast Cancer Cells ◽  
Green Tea Extract ◽  
Hexane Fraction ◽  
Phytochemical Screening ◽  
Tea Extract

Green tea (Camellia sinensis) is one of traditional plants that have the potential as an anticancer. The sample used in this research commercial green tea extract. The purpose of this study was to test the antiproliferation activity of green tea extract on breast cancer cell MCM-B2 in vitro. Green tea extract fractionated using three solvents, ie water, ethanol 70%, and n-hexane. Extract and fraction of green tea water have value Lethality Concentration 50 (LC50) more than 1000 ppm. The fraction of ethanol 70% and n-hexane had an LC50 value of 883.48 ppm and 600.56 ppm, respectively. The results of the phytochemical screening of green tea extract are flavonoids, tannins, and saponins, while the phytochemical screening results of n-hexane fraction are flavonoids and tannins. Antiproliferation activity was tested on breast cancer cells MCM-B2 and normal cells Vero by trypan blue staining method. The highest MCM-B2 cell inhibitory activity was achieved at a concentration of 13000 ppm green tea extract and 1000 ppm of n-hexane fraction, 59% and 59%, respectively. The extract and n-hexane fraction of green tea are not toxic to normal Vero cells characterized by not inhibiting normal cell proliferation. Keywords: antiproliferative, cancer cell MCM-B2, commercial green tea, cytotoxicity

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