Oral administration of Dictyostelium differentiation-inducing factor 1 lowers blood glucose levels in streptozotocin-induced diabetic rats

Life Sciences ◽  
2016 ◽  
Vol 155 ◽  
pp. 56-62 ◽  
Author(s):  
Ritsuko Kawaharada ◽  
Akio Nakamura ◽  
Katsunori Takahashi ◽  
Haruhisa Kikuchi ◽  
Yoshiteru Oshima ◽  
...  
Keyword(s):  
Blood Glucose ◽  
Oral Administration ◽  
Diabetic Rats ◽  
Glucose Levels ◽  
Blood Glucose Levels

Evaluation of Glucose and Lipid Lowering Activity of Arganimide A in Normal and Streptozotocin-Induced Diabetic Rats

2019 ◽  
Vol 19 (4) ◽  
pp. 503-510 ◽  
Author(s):  
Mohamed Eddouks ◽  
Farid Khallouki ◽  
Robert W. Owen ◽  
Morad Hebi ◽  
Remy Burcelin
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Oral Administration ◽  
Lipid Profile ◽  
Plasma Cholesterol ◽  
Diabetic Rats ◽  
Lipid Lowering ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Lowering Activity

Aims: Arganimide A (4,4-dihydroxy-3,3-imino-di-benzoic acid) is a compound belonging to a family of aminophenolics found in fruit of Argania spinosa. The purpose of this study was to investigate the glucose and lipid lowering activity of Arganimide A (ARG A). Methods: The effect of a single dose and daily oral administration of Arganimide A (ARG A) on blood glucose levels and plasma lipid profile was tested in normal and streptozotocin (STZ) diabetic rats at a dose of 2 mg/kg body weight. Results: Single oral administration of ARG A reduced blood glucose levels from 26.50±0.61 mmol/L to 14.27±0.73 mmol/L (p<0.0001) six hours after administration in STZ diabetic rats. Furthermore, blood glucose levels were decreased from 5.35±0.30 mmol/L to 3.57±0.17 mmol/L (p<0.0001) and from 26.50±0.61 mmol/L to 3.67±0.29 mmol/L (p<0.0001) in normal and STZ diabetic rats, respectively, after seven days of treatment. Moreover, no significant changes in body weight in normal and STZ rats were shown. According to the lipid profile, the plasma triglycerides levels were decreased significantly in diabetic rats after seven days of ARG treatment (p<0.05). Moreover, seven days of ARG A treatment decreased significantly the plasma cholesterol concentrations (p<0.001). Conclusion: ARG A possesses glucose and lipid-lowering activity in diabetic rats and this natural compound may be beneficial in the treatment of diabetes.

scholarly journals Physiological study to investigate the activity of an aqueous extract ofCinnamomum cassiabark on the blood glucose levels in healthy and diabetic rats induced by streptozotocin (stz)

2016 ◽  
Vol 13 (4) ◽  
pp. 681-693
Author(s):  
Baghdad Science Journal
Keyword(s):  
Blood Glucose ◽  
Oral Administration ◽  
Aqueous Extract ◽  
Diabetic Rats ◽  
Physiological Study ◽  
Cinnamomum Cassia ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Single Oral Administration

The present study was investigated the activity of aqueous extract from Cinnamomum cassia bark on the blood glucose levels in healthy and diabetic rats induced by Streptozotcin (STZ). In healthy rats the blood glucose levels were slightly decreased after six hoursof single oral administration with dose (25 mg/kg) of body wight, as well as four weeks after twice daily repeated oral administration of aqueous extract of Cinnamomum cassia bark. In streptozotocin induced diabetic rats we absorved high significant decreased (p

scholarly journals Leaf Aqueous Extract of Argania spiniosa Exhibits Antihyperglycemic Effect in Diabetic Rats

2019 ◽  
Vol 17 (1) ◽  
pp. 64-71 ◽  
Author(s):  
Morad Hebi ◽  
Mohamed Eddouks
Keyword(s):  
Antioxidant Activity ◽  
Blood Glucose ◽  
Oral Administration ◽  
Aqueous Extract ◽  
Diabetic Rats ◽  
Oral Glucose ◽  
Argania Spinosa ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Leaf Aqueous Extract

Introduction: Argania Spinosa L. (Sapotaceae) is an endemic species from south-western Morocco. This plant has many traditional uses including its use in the treatment of diabetes. Objective: The objective of the study was to evaluate the antidiabetic activity of Argania Spinosa Leaf Aqueous Extract (A.S.L.A.E). Methods: The antidiabetic effect of A.S.L.A.E was evaluated in both normal and streptozotocin (STZ)-induced diabetic rats treated at a dose of 20 mg/kg body weight for 15 days. The histopathological changes in the liver were evaluated. In addition, the antioxidant activity of this extract was also studied. Results: Single oral administration of A.S.L.A.E (20 mg/kg) showed no significant change in blood glucose levels in both normal and STZ induced diabetic rats after 6 hours of administration. Furthermore, in normal rats, repeated oral administration of A.S.L.A.E reduced blood glucose levels. Moreover, blood glucose levels decreased in STZ diabetic rats after fifteen days of treatment. According to the oral glucose tolerance test, the A.S.L.A.E (20 mg/kg) was shown to prevent significantly the increase in blood glucose levels in normal treated rats. Moreover, A.S.L.A.E showed antioxidant activity. Conclusion: The results show that Argania spinosa leaf aqueous extract possesses significant antihyperglycemic activity.

Antihyperglycemic Effect of the Moroccan Collard Green (Brassica oleracea var. viridis) in Streptozotocin-Induced Diabetic Rats

2020 ◽  
Vol 20 ◽  
Author(s):  
Ayoub Amssayef ◽  
Mohamed Eddouks
Keyword(s):  
Antioxidant Activity ◽  
Blood Glucose ◽  
Oral Administration ◽  
Brassica Oleracea ◽  
Aqueous Extract ◽  
Diabetic Rats ◽  
Antihyperglycemic Effect ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Collard Green

Aims: The aim of the study was to assess the antihyperglycemic activity of Brassica oleracea. Background: Collard green or Brassica oleracea var. viridis which belongs to Brassicaceae family is ranked at 10th place of “powerhouse” vegetables, and it is commonly used traditionally for the treatment of diabetes in Morocco. Objective: This current investigation aimed to assess the antihyperglycemic capacity of the aqueous extract of Collard green leaves. Methods: The effect of a single (6 hours) and repeated (seven days of treatment) oral administration of Collard green aqueous extract (CGAE) at a dose of 60 mg/kg on glucose and lipid profile was examined in normal and streptozotocin-induced diabetic rats. Additionally, histopathological examination of liver was carried out according to the Hematoxylin-Eosin method. Furthermore, a preliminary phytochemical screening, and the quantification of phenolic, flavonoid and tannins contents as well as the antioxidant activity using DPPH assay were carried out. Results: The results demonstrated that Collard green A.E. (CGAE) exhibited a significant antihyperglycemic effect and positive improvement liver histology in diabetic rats. After a single oral administration of CGAE, blood glucose levels were lowered from 15±2 mM to 4.9±1 mM (p<0.0001) at the sixth hour in diabetic rats but no change was observed in normal rats. Repeated CGAE administration was able to reduce blood glucose levels from 15±2 mM to normal values (5±1 mM at the seventh day, p<0.0001) while the extract had no effect in normal rats. In addition, we revealed that CGAE is rich in several phytochemical compounds and exerts an important antioxidant activity. Conclusion: In conclusion, this study demonstrates that Collard green possesses a beneficial effect against diabetes, and can be used in the program diet of diabetic patients.

scholarly journals Effect of Terebinthus atlanticus on Glucose Metabolism in Diabetic Rats

2020 ◽  
Vol 20 (1) ◽  
pp. 31-40
Author(s):  
Fadwa El-Ouady ◽  
Lhoussaine Hajji ◽  
Mohamed Eddouks
Keyword(s):  
Blood Glucose ◽  
Oral Administration ◽  
Aqueous Extract ◽  
Antioxidant Activities ◽  
Histopathological Examination ◽  
Diabetic Rats ◽  
Control Group ◽  
Histopathological Analysis ◽  
Glucose Levels ◽  
Blood Glucose Levels

Background:: Terebinthus atlanticus (Anacardiaceae) is an important source of essential oil and phenolic compounds justifying its use in traditional medicine. Objective:: The present work aimed to evaluate the antidiabetic and the antioxidant activities of the aqueous extract of the leaves of Terebinthus atlanticus (T. atlanticus). Methods:: The current study evaluated the effect of a single and repeated (15 days of treatment) oral administration of the aqueous extract of the leaves of T. atlanticus (PALAE) on blood glucose levels in normal and streptozotocin(STZ)-induced diabetic rats. Furthermore, the effect of PALAE on glucose tolerance and histopathological examination of the liver was carried out. Results:: A single oral administration of PALAE reduced blood glucose levels in normal (p<0.05), and STZ diabetic rats (p<0.0001), 6 and 4 hours after administration, respectively. Furthermore, this extract had an optimal effect (p<0.0001) in both normal and STZ diabetic rats at the 15th and 7th day of treatment. This extract was also shown to prevent significantly the increase on blood glucose levels 120 min after glucose administration, in both normal (p<0.05), and diabetic (p<0.01) treated rats when compared to the control group. In addition, the histopathological analysis highlighted the positive effect of T. atlanticus on pancreas and liver. Conclusion:: The study demonstrates the antihyperglycemic effect of the aqueous T. atlanticus extracts in diabetic rats which should be mediated through the amelioration of the oxidative stress as well as an improvement in liver histology.

Antihyperglycemic Activity of Micromeria Graeca Aqueous Extract in Streptozotocin-Induced Diabetic Rats

2020 ◽  
Vol 20 ◽  
Author(s):  
Mourad Akdad ◽  
Amine Azzane ◽  
Fadwa El Ouady ◽  
Abdelhadi Moujani ◽  
Farid El Khallouki ◽  
...  
Keyword(s):  
Blood Glucose ◽  
Oral Administration ◽  
Antioxidant Capacity ◽  
Aqueous Extract ◽  
Diabetic Rats ◽  
Phytochemical Analysis ◽  
Dependent Manner ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Dpph Method

Aims: This study aimed to investigate the effect of Micromeria graeca on blood glucose levels and lipid parameters in an experimental model of diabetes. Objective: This study aimed to evaluate the antihyperglycemic and antihyperlipidemic effects of the aqueous extract of Micromeria graeca (M. graeca) aerial parts (AEMG) under physiological (normal rats) and pathological (STZ-induced diabetic rats) conditions. Additionally, we analyzed the phytochemical composition and antioxidant capacity. Methods: The effects of the acute and sub-chronic administration of AEMG (20 mg/kg) on blood glucose levels and lipid profiles were evaluated in normal and streptozotocin-induced diabetic rats. Moreover, the phytochemical analysis was carried with standard tests and estimation of total phenolics compounds by Folin–Ciocalteu reagent. The antioxidant activity was realized by the DPPH method. Results: Single oral administration of M. greaca aqueous extract decreased blood glucose levels 4 and 6 hours (p<0.01) after treatment in diabetic rats. In accordance, the repeated oral administration of M. graeca showed a significant reduction in blood glucose levels in diabetic rats since the second day to the end of the period experiment (p<0.0001). In addition, two weeks of treatment with M. graeca reduced total cholesterol levels (p<0.05) with a significant increase of HDL-c level (p<0.01) in diabetic rats. Moreover, M. graeca scavenged DPPH radical in a dose-dependent manner (IC50=0.48 mg/ml), whereas IC50 was 0.55 mg/ml for BHT. Phytochemical analysis showed the richness of Micromeria graeca on polyphenols (281.94±4.61 mg GAE/1 g), flavonoids, tannins, glycosides, saponins, sterols, sesquiterpenes, and terpenoids. Conclusion: AEMG exhibits antihyperglycemic and antihyperlipidemic activities in STZ-induced diabetic rats and a potent antioxidant capacity.

Antihyperglycemic activity and safety assessment of the aqueous extract of Scorzonera undulata ssp deliciosa aerial parts in rat

2020 ◽  
Vol 20 ◽  
Author(s):  
Mohammed Ajebli ◽  
Ayoub Amssayef ◽  
Mohamed Eddouks
Keyword(s):  
Blood Glucose ◽  
Acute Toxicity ◽  
Oral Administration ◽  
Aqueous Extract ◽  
Glycogen Content ◽  
Diabetic Rats ◽  
Antihyperglycemic Effect ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Phytochemical Investigation

Aims: The aim of this study was to evaluate the antidiabetic effect of Scorzonera undulata. Background: Scorzonera undulata ssp deliciosa, locally known as “Guiz”, is used as a phytomedicine in Morocco and Algeria to treat different health problems. Interestingly, it is used in the Moroccan pharmacopeia to treat diabetes. To our knowledge, this medicinal herb has never been investigated for any pharmacological activity. Objective: The aim of this study was to evaluate the antihyperglycemic effect of the aqueous extract of the aerial part of Scorzonera undulata (SUAP) in normal and STZ-induced diabetic rats and to assess the acute toxicity of this extract in Wistar rats. Methods: This study investigated the effects of SUAP at a dose of 20 mg/kg on blood glucose levels in normal and streptozotocin(STZ)- induced diabetic rats. The acute toxicity of SUAP was examined according to the OECD test guideline; rats were divided into four groups of each sex and orally received the SUAP (1000, 2000, or 3000 mg/kg BW). Post-treatment, body weight, signs of toxicity, and/or mortality were observed during 14 days. Other assays such as histopathological examination, preliminary phytochemical investigation, determination of glycogen content and evaluation of α-amylase were performed according to standard protocols. Results: Findings of the current study depicted that both single and repeated oral administration of SUAP (20 mg/kg) generated significant fall in the blood glucose levels in diabetic rats. A single oral administration of SUAP (at highest dose of 3000 mg/kg BW) had no significant acute toxicological effects, and oral LD50 of SUAP was greater than 3000 mg/kg. Furthermore, repeated oral administration of SUAP during 15 days led to increase the liver glycogen content in diabetic rats, to improve the histopathological structure of the liver and pancreas in SUAP-treated diabetic rats and to ameliorate some biochemical parameters such as ALT and creatinine. SUAP had no effect on αamylase activity. In addition, the preliminary phytochemical investigation showed the richness of the roots of SUAP in some phytochemicals, particularly the polyphenols. Conclusion: The present study demonstrates the antihyperglycemic effect of Scorzonera undulata in diabetic rats which could be involved through improvement of liver structure and function. In addition, the dose used is not toxic. Finally, the extract contains large amounts of bioactive compounds, mainly polyphenols.

Aqueous Asteriscus graveolens Extract Exhibits Antidiabetic and Hepatoprotective Effects in Diabetic Rats

2020 ◽  
Vol 10 (4) ◽  
pp. 459-466
Author(s):  
Fadwa El-Ouady ◽  
Mohamed Eddouks
Keyword(s):  
Antioxidant Activity ◽  
Blood Glucose ◽  
Oral Administration ◽  
Aerial Part ◽  
Diabetic Rats ◽  
Antidiabetic Effect ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
In Vitro Antioxidant Activity

Background: Asteriscus graveolens (A. graveolens) is a medicinal plant with numerous applications in phytotherapy including diabetes management. However, the antidiabetic effect of this plant has never been investigated experimentally. Objective: The objective of this study was to evaluate the antidiabetic effect of aqueous extract prepared from the aerial part of Asteriscus graveolens (A. graveolens) in normal and STZ-induced diabetic rats. Method: The effect of a single dose and daily oral administration for 15 days of the aerial part aqueous extract of A. graveolens (AGAPE) (10 mg/kg) on blood glucose levels was evaluated. In addition, histopathological examination of the liver as well as the in vitro antioxidant activity AGAPE were performed. Results: The data illustrate that both single and repeated oral administration of AGAPE were able to reduce blood glucose levels in normal and diabetic rats. The extract ameliorated histopathological properties of liver in diabetic rats and it exhibited in vitro antioxidant activity. Conclusion: In conclusion, the present investigation revealed for the first time that A. graveolens possesses potent antidiabetic and hepatoprotective activities which support the traditional claim of the plant.

Mentha pulegium Aqueous Extract Exhibits Antidiabetic and Hepatoprotective Effects in Streptozotocin-Induced Diabetic Rats

2019 ◽  
Vol 19 (3) ◽  
pp. 292-301
Author(s):  
Omar Farid ◽  
Naoufel Ali Zeggwagh ◽  
Fadwa EL Ouadi ◽  
Mohamed Eddouks
Keyword(s):  
Blood Glucose ◽  
Glucose Tolerance ◽  
Aqueous Extract ◽  
Morphometric Analysis ◽  
Diabetic Rats ◽  
Mentha Pulegium ◽  
Glucose Levels ◽  
Histological Sections ◽  
Blood Glucose Levels ◽  
Liver And Pancreas

Objective: The aim of this work was to evaluate the antihyperglycemic activity of aerial parts aqueous extract (A.P.A.E) of Mentha pulegium (M. pulegium) on blood glucose levels in normal and streptozotocin(STZ)-induced diabetic rat. The glucose tolerance was evaluated in normal rats. Moreover, the histological sections and morphometric analysis at the liver and pancreas have been carried out in this investigation both in normal and STZ-diabetic rats. Methods: The effect of A.P.A.E of M. pulegium (20 mg/kg) on blood glucose levels was investigated in normal and diabetic rats (n=6). Histopathological changes in liver and pancreas were examined under phase contrast microscope and a preliminary screening for various bioactive constituents was realized according to standard methods. Key Findings: Both single and repeated oral administration of A.P.A.E (20 mg/kg) caused a significant reduction in blood glucose levels in STZ-diabetic rats (p<0.0001). The morphometric analysis and histological sections realized in pancreas and liver have showed the beneficial effect of the A.P.A.E in cellular population. According to oral glucose tolerance test (OGTT), the aqueous extract has revealed an improvement of glucose tolerance in normal rat. Furthermore, the preliminary phytochemical screening of A.P.A.E of M. pulegium has demonstrated the presence of various metabolite compounds including polyphenols, flavonoids, terpenoids tannins, cyanidins, sesquiterpenes, and glycosides. Conclusion: We conclude that the A.P.A.E of M. pulegium (20 mg/kg) exhibits a potent antihyperglycemic activity in STZ diabetic rats.

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