Low-molecular weight chitosan/vascular endothelial growth factor short hairpin RNA for the treatment of hepatocellular carcinoma

Life Sciences ◽  
2012 ◽  
Vol 91 (23-24) ◽  
pp. 1207-1215 ◽  
Author(s):  
Zhen Huang ◽  
Lei Dong ◽  
Jijun Chen ◽  
Fengbo Gao ◽  
Zhengping Zhang ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Molecular Weight ◽  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
Low Molecular Weight ◽  
Vascular Endothelial ◽  
Hairpin Rna ◽  
Low Molecular Weight Chitosan ◽  
Short Hairpin ◽  
Endothelial Growth Factor

scholarly journals Use of Proximity Ligation to Screen for Inhibitors of Interactions between Vascular Endothelial Growth Factor A and Its Receptors

2008 ◽  
Vol 54 (7) ◽  
pp. 1218-1225 ◽  
Author(s):  
Sigrun M Gustafsdottir ◽  
Stefan Wennström ◽  
Simon Fredriksson ◽  
Edith Schallmeiner ◽  
Andrew D Hamilton ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
Dose Response ◽  
Dna Aptamer ◽  
Low Molecular Weight ◽  
Vascular Endothelial ◽  
Proximity Ligation ◽  
Receptor Complexes ◽  
Endothelial Growth Factor

Abstract Background: Improved methods are required to screen drug candidates for their influences on protein interactions. There is also a compelling need for miniaturization of screening assays, with attendant reductions in reagent consumption and assay costs. Methods: We used sensitive, miniaturized proximity ligation assays (PLAs) to monitor binding of vascular endothelial growth factor A (VEGF-A) to 2 of its receptors, VEGFR-1 and VEGFR-2. We measured the effects of proteins and low molecular weight compounds capable of disrupting these interactions and compared the results with those obtained by immunoblot analysis. We analyzed 6 different inhibitors: a DNA aptamer, a mixed DNA/RNA aptamer, a monoclonal VEGF-A neutralizing antibody, a monoclonal antibody directed against VEGFR-2, a recombinant competitive protein, and a low molecular weight synthetic molecule. Results: The PLAs were successful for monitoring the formation and inhibition of VEGF-A–receptor complexes, and the results correlated well with those obtained by measuring receptor phosphorylation. The total PLA time is just 3 hours, with minimal manual work and reagent additions. The method allows evaluation of the apparent affinity [half-maximal inhibitory concentration (IC50)] from a dose–response curve. Conclusions: The PLA may offer significant advantages over conventional methods for screening the interactions of ligands with their receptors. The assay may prove useful for parallel analyses of large numbers of samples in the screening of inhibitor libraries for promising agents. The technique provides dose–response curves, allowing IC50 values to be calculated.

Chitosan/Short Hairpin RNA Complexes for Vascular Endothelial Growth Factor Suppression Invasive Breast Carcinoma

2010 ◽  
Vol 20 (4) ◽  
pp. 183-190 ◽  
Author(s):  
Emine Şalva ◽  
Levent Kabasakal ◽  
Fatih Eren ◽  
Fulya Çakalağaoğlu ◽  
Naziye Özkan ◽  
...  
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
Breast Carcinoma ◽  
Short Hairpin Rna ◽  
Invasive Breast Carcinoma ◽  
Vascular Endothelial ◽  
Hairpin Rna ◽  
Short Hairpin ◽  
Endothelial Growth Factor ◽  
Rna Complexes
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