Molecular diagnosis of α-thalassemia by combining real-time PCR with SYBR Green1 and dissociation curve analysis

2006 ◽  
Vol 148 (1) ◽  
pp. 6-12 ◽  
Author(s):  
Jingzhong Liu ◽  
Mei Yan ◽  
Zhangyong Wang ◽  
Lirong Wang ◽  
Yan Zhou ◽  
...  
Keyword(s):  
Real Time ◽  
Molecular Diagnosis ◽  
Real Time Pcr ◽  
Curve Analysis ◽  
Dissociation Curve

Improvement in the Detection of α0- and Deletional α-Thalassemia by Real-Time PCR Combined with Dissociation Curve Analysis

2009 ◽  
Vol 122 (1) ◽  
pp. 17-22 ◽  
Author(s):  
Jingzhong Liu ◽  
Ning Tang ◽  
Quanzhang Liu ◽  
Lirong Wang ◽  
Han Han ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Curve Analysis ◽  
Dissociation Curve ◽  
Alpha Thalassemia

scholarly journals Multiplex Real-time PCR Detection and Differentiation of Colletotrichum Species Infecting Soybean

Plant Disease ◽  
2015 ◽  
Vol 99 (11) ◽  
pp. 1559-1568 ◽  
Author(s):  
Hui-Ching Yang ◽  
James S. Haudenshield ◽  
Glen L. Hartman
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
The United States ◽  
Curve Analysis ◽  
Melting Curve Analysis ◽  
Species Differentiation ◽  
Cox1 Gene ◽  
Dissociation Curve ◽  
Colletotrichum Species ◽  
Pcr Assays

Colletotrichum species are fungal plant pathogens of worldwide significance. Colletotrichum species were isolated from soybean with anthracnose symptoms in five states in the United States from 2009 to 2013. Among 240 isolates collected, four Colletotrichum species were initially identified by morphological and sequence analysis, including C. chlorophyti, C. incanum, C. truncatum, and Colletotrichum sp. (henceforth Glomerella glycines, the name of its sexual state). To increase diagnostic efficiency and accuracy, real-time multiplex PCR assays based on a double-stranded DNA-binding dye coupled with dissociation curve analysis were designed, using a region of the cytochrome c oxidase subunit 1 (cox1) gene to discern these four Colletotrichum species. Two sets of duplex, real-time PCR assays were established and species differentiation was based upon amplicon melting point temperatures (Tm) in the dissociation curve analysis. The Set 1 duplex assay distinguished C. chlorophyti and G. glycines, and the Set 2 duplex assay distinguished C. incanum and C. truncatum. Successful detection was achieved with as little as 1 pg DNA. The assays were especially useful for differentiating C. chlorophyti, C. incanum, and C. truncatum, which have similar morphological features. Colletotrichum gloeosporioides, another pathogen associated with soybean anthracnose, was not resolved from G. glycines by the melting curve analysis. The two duplex real-time PCR assays were used to screen more than 200 purified Colletotrichum isolates, showing that they were rapid and effective methods to detect and differentiate Colletotrichum species infecting soybean.

scholarly journals Rapid genotypic detection ofBacillus anthracisand theBacillus cereusgroup by multiplex real-time PCR melting curve analysis

2005 ◽  
Vol 43 (2) ◽  
pp. 301-310 ◽  
Author(s):  
Kijeong Kim ◽  
Juwon Seo ◽  
Katherine Wheeler ◽  
Chulmin Park ◽  
Daewhan Kim ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Melting Curve ◽  
Curve Analysis ◽  
Melting Curve Analysis

Ultrafast real-time PCR with integrated melting curve analysis and duplex capacities using a low-cost polymer lab-on-a-chip system

2015 ◽  
Author(s):  
Rainer Gransee ◽  
Tristan Schneider ◽  
Deniz Elyorgun ◽  
Xenia Strobach ◽  
Tobias Schunck ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Low Cost ◽  
Melting Curve ◽  
Lab On A Chip ◽  
Curve Analysis ◽  
Melting Curve Analysis

Determination of Helicobacter pylori cagA, vacA genotypes with real-time PCR melting curve analysis

2001 ◽  
Vol 95 (1-6) ◽  
pp. 369-377 ◽  
Author(s):  
Agnes Ruzsovics ◽  
Bela Molnar ◽  
Zsuzsa Unger ◽  
Zsolt Tulassay ◽  
Laszlo Pronai
Keyword(s):  
Helicobacter Pylori ◽  
Real Time ◽  
Real Time Pcr ◽  
Melting Curve ◽  
Curve Analysis ◽  
Melting Curve Analysis

scholarly journals Rapid plasmid replicon typing by real time PCR melting curve analysis

2013 ◽  
Vol 13 (1) ◽  
pp. 83 ◽  
Author(s):  
Maikel Boot ◽  
Susanne Raadsen ◽  
Paul HM Savelkoul ◽  
Christina Vandenbroucke-Grauls
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Melting Curve ◽  
Curve Analysis ◽  
Melting Curve Analysis ◽  
Plasmid Replicon
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