Platelet-activating factor (PAF) increase intracellular lipid accumulation by increasing both LDL and scavenger receptors in human mesangial cells

2006 ◽  
Vol 147 (6) ◽  
pp. 281-289 ◽  
Author(s):  
Elizabeth Fragopoulou ◽  
Christos Iatrou ◽  
Smaragdi Antonopoulou ◽  
Xiong-Zhong Ruan ◽  
Ray L. Fernando ◽  
...  
Keyword(s):  
Lipid Accumulation ◽  
Mesangial Cells ◽  
Platelet Activating Factor ◽  
Scavenger Receptors ◽  
Intracellular Lipid ◽  
Human Mesangial Cells ◽  
Intracellular Lipid Accumulation

scholarly journals Association of intracellular lipid accumulation in subcutaneous adipocyte precursors and plasma adipokines in bariatric surgery candidates

2019 ◽  
Vol 18 (1) ◽  
Author(s):  
Ioana Hristov ◽  
Veronica Mocanu ◽  
Florin Zugun-Eloae ◽  
Luminita Labusca ◽  
Iustina Cretu-Silivestru ◽  
...  
Keyword(s):  
Bariatric Surgery ◽  
Lipid Accumulation ◽  
Intracellular Lipid ◽  
Intracellular Lipid Accumulation

Dysregulation of low-density lipoprotein receptor contributes to podocyte injuries in diabetic nephropathy

2015 ◽  
Vol 308 (12) ◽  
pp. E1140-E1148 ◽  
Author(s):  
Yang Zhang ◽  
Kun Ling Ma ◽  
Jing Liu ◽  
Yu Wu ◽  
Ze Bo Hu ◽  
...  
Keyword(s):  
High Glucose ◽  
Lipid Accumulation ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Low Density ◽  
Lipoprotein Receptor ◽  
Intracellular Lipid ◽  
Phenotypic Alteration ◽  
Density Lipoprotein Receptor ◽  
Intracellular Lipid Accumulation

Dyslipidemia plays crucial roles in the progression of diabetic nephropathy (DN). This study investigated the effects of high glucose on lipid accumulation in podocytes and explored its underlying mechanisms. Male db/m and db/db mice were fed a normal chow diet for 8 wk. Immortalised mouse podocytes were treated with or without high glucose for 24 h. The changes to the morphology and ultramicrostructures of the kidneys in mice were examined using pathological staining and electron microscopy. Intracellular lipid accumulation was evaluated by Oil Red O staining and a free cholesterol quantitative assay. The expressions of the molecules involved in low-density lipoprotein receptor (LDLr) pathway and podocyte injury were examined using immunofluorescent staining, real-time PCR, and Western blot. There were increased levels of plasma lipid, serum creatinine, and proteinuria in db/db mice compared with db/m mice. Moreover, there was significant mesangial matrix expansion, basement membrane thickening, podocyte foot process effacement, and phenotypic alteration in the db/db group. Additionally, lipid accumulation in the kidneys of db/db mice was increased due to increased protein expressions of LDLr, sterol regulatory element-binding protein (SREBP) cleavage-activating protein, and SREBP-2. These effects were further confirmed by in vitro studies. Interestingly, the treatment with LDLr siRNA inhibited lipid accumulation in podocytes and decreased the protein expression of molecules associated with phenotypic alteration in podocytes. High glucose disrupted LDLr feedback regulation in podocytes, which may cause intracellular lipid accumulation and alteration of podocyte phenotype, thereby accelerating DN progression.

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