scholarly journals Use of hyperspectral imaging to assess endothelial dysfunction in peripheral arterial disease

2016 ◽  
Vol 64 (4) ◽  
pp. 1066-1073 ◽  
Author(s):  
Brandon J. Sumpio ◽  
Gianluca Citoni ◽  
Jason A. Chin ◽  
Bauer E. Sumpio
Keyword(s):  
Endothelial Dysfunction ◽  
Peripheral Arterial Disease ◽  
Hyperspectral Imaging ◽  
Arterial Disease ◽  
Peripheral Arterial

Abstract 17797: Noninvasive Optical Imaging of Response to On-Demand Antioxidant Therapy in a Model of Peripheral Arterial Disease

Circulation ◽  
2014 ◽  
Vol 130 (suppl_2) ◽  
Author(s):  
Kristin M Poole ◽  
Christopher E Nelson ◽  
John R Martin ◽  
Devin R McCormack ◽  
Rucha V Joshi ◽  
...  
Keyword(s):  
Peripheral Arterial Disease ◽  
Hyperspectral Imaging ◽  
Time Course ◽  
Arterial Disease ◽  
Diameter Distribution ◽  
Novel Therapy ◽  
On Demand ◽  
Vessel Morphology ◽  
Peripheral Arterial

Peripheral arterial disease is often modeled by surgical induction of hind limb ischemia (HLI) in mice to study collateral vessel development. However, there is a need for methodologies that provide intravital, multifunctional, quantitative data on ischemic recovery for robust evaluation of new therapeutics. Here, we apply hyperspectral imaging and optical coherence tomography (OCT) to longitudinally assess hemoglobin oxygen saturation (SaO 2 ) and vessel morphology in response to a novel therapy. Injectable microspheres loaded with curcumin were synthesized from reactive oxygen species (ROS)-responsive poly(propylene) sulfide (PPS) to provide “on demand”, local release of the antioxidant drug curcumin to reduce tissue-damaging oxidative stress in a mouse model of diabetic HLI. Curcumin-PPS microspheres significantly reduced intracellular ROS in LPS-activated RAW 264.7 macrophages and rescued viability of 3T3 fibroblasts treated with cytotoxic levels of H 2 O 2 in vitro . HLI was induced in FVB mice with streptozotocin-induced diabetes, and curcumin-PPS or blank-PPS microspheres were injected into the ischemic limb. Curcumin-PPS significantly improved recovery of SaO 2 in the ischemic footpad relative to blank-PPS and vehicle (saline) groups over a one week time course evaluated with hyperspectral imaging (n≥8/group, p<0.05). Vessel structure in the gastrocnemius was imaged noninvasively with OCT at day 7 (Figure), revealing trends toward increased vessel area density and vessel length fraction in the curcumin-PPS group (n=5/group). The vessel diameter distribution was also extracted from OCT data. Our collective data indicate that sustained, on demand curcumin delivery has significant therapeutic promise for improving ischemic tissue recovery. Also, the hyperspectral and OCT imaging methods showcased provide quantitative, noninvasive monitoring of vasculature and can accelerate screening of novel therapies.

In concomitant coronary and peripheral arterial disease, inflammation of the affected limbs predicts coronary artery endothelial dysfunction

2008 ◽  
Vol 201 (2) ◽  
pp. 440-446 ◽  
Author(s):  
Gregorio Brevetti ◽  
Federico Piscione ◽  
Plinio Cirillo ◽  
Gennaro Galasso ◽  
Vittorio Schiano ◽  
...  
Keyword(s):  
Coronary Artery ◽  
Endothelial Dysfunction ◽  
Peripheral Arterial Disease ◽  
Arterial Disease ◽  
Peripheral Arterial

scholarly journals Endothelial dysfunction and peripheral arterial disease

2007 ◽  
Vol 28 (15) ◽  
pp. 1910-1910
Author(s):  
M. Frick ◽  
A. Suessenbacher ◽  
H. F. Alber ◽  
O. Pachinger
Keyword(s):  
Endothelial Dysfunction ◽  
Peripheral Arterial Disease ◽  
Arterial Disease ◽  
Peripheral Arterial

scholarly journals Quantitative optical imaging of vascular response in vivo in a model of peripheral arterial disease

2013 ◽  
Vol 305 (8) ◽  
pp. H1168-H1180 ◽  
Author(s):  
Kristin M. Poole ◽  
Jason M. Tucker-Schwartz ◽  
Wesley W. Sit ◽  
Alex J. Walsh ◽  
Craig L. Duvall ◽  
...  
Keyword(s):  
Blood Flow ◽  
Peripheral Arterial Disease ◽  
Optical Imaging ◽  
Hyperspectral Imaging ◽  
Temporal Dynamics ◽  
Arterial Disease ◽  
Sensor Data ◽  
Morphological Data ◽  
Non Invasive ◽  
Peripheral Arterial

The mouse hind limb ischemia (HLI) model is well established for studying collateral vessel formation and testing therapies for peripheral arterial disease, but there is a lack of quantitative techniques for intravitally analyzing blood vessel structure and function. To address this need, non-invasive, quantitative optical imaging techniques were developed to assess the time-course of recovery in the mouse HLI model. Hyperspectral imaging and optical coherence tomography (OCT) were used to non-invasively image hemoglobin oxygen saturation and microvessel morphology plus blood flow, respectively, in the anesthetized mouse after induction of HLI. Hyperspectral imaging detected significant increases in hemoglobin saturation in the ischemic paw as early as 3 days after femoral artery ligation ( P < 0.01), and significant increases in distal blood flow were first detected with OCT 14 days postsurgery ( P < 0.01). Intravital OCT images of the adductor muscle vasculature revealed corkscrew collateral vessels characteristic of the arteriogenic response to HLI. The hyperspectral imaging and OCT data significantly correlated with each other and with laser Doppler perfusion imaging (LDPI) and tissue oxygenation sensor data ( P < 0.01). However, OCT measurements acquired depth-resolved information and revealed more sustained flow deficits following surgery that may be masked by more superficial measurements (LDPI, hyperspectral imaging). Therefore, intravital OCT may provide a robust biomarker for the late stages of ischemic limb recovery. This work validates non-invasive acquisition of both functional and morphological data with hyperspectral imaging and OCT. Together, these techniques provide cardiovascular researchers an unprecedented and comprehensive view of the temporal dynamics of HLI recovery in living mice.

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