scholarly journals PS204. Mechanical (or “Gravitational”) Unloading Reduces Inflammatory and Cell Adhesion Molecule Gene Expression in Human Endothelial Cells

2012 ◽  
Vol 55 (6) ◽  
pp. 78S-79S
Author(s):  
Marlene Grenon ◽  
Jesus Aguado-Zunega ◽  
Michael S. Conte ◽  
Millie Hughes-Fulford
Keyword(s):  
Gene Expression ◽  
Endothelial Cells ◽  
Cell Adhesion ◽  
Adhesion Molecule ◽  
Cell Adhesion Molecule ◽  
Human Endothelial Cells ◽  
Gravitational Unloading ◽  
Adhesion Molecule Gene

Abstract 353: Mechanical (or “Gravitational”) Unloading Reduces Inflammatory and Cell Adhesion Molecule Gene Expression in Human Endothelial Cells

2012 ◽  
Vol 32 (suppl_1) ◽  
Author(s):  
S Marlene Grenon ◽  
Jesus Aguado-Zuniga ◽  
Michael Conte ◽  
Millie Hughes-Fulford
Keyword(s):  
Gene Expression ◽  
Endothelial Cells ◽  
Adhesion Molecule ◽  
Cell Function ◽  
Quantitative Polymerase Chain Reaction ◽  
Cellular Interactions ◽  
Gravitational Unloading ◽  
Experimental Conditions ◽  
Adhesion Molecule Gene ◽  
The Impact

Objectives: Mechanical forces including gravity affect mechanotransduction and subsequent cell function. The goal of this study was to investigate the impact of mechanical unloading (MU) and loading (ML) of endothelial cells (ECs) with microgravity and hypergravity respectively, with the hypothesis that MU alters expression of inflammatory and adhesion molecule gene expression and these changes are reversed by ML. Methods: Human umbilical vascular endothelial cells (HUVECs) grown to confluency were studied. A desktop random positioning machine and a gravitational cell-loading apparatus provided MU and ML conditions, respectively. The experimental conditions included: 1) controls exposed to 1-gravity environment for 24 h (CL), 2) MU for 24 hours, 3) MU for 24 hours with three 30-minutes periods of ML of 12-gravity (MU/ML). Gene expression was studied with reverse transcription followed by real-time quantitative polymerase chain reaction (qRTPCR). Results: MU led to a significant decrease in gene expression of the adhesion molecules ICAM-1, VCAM-1, E-Selectin, as well as TNF-α, IL-6 and VEGF. In contrast, NOS-3, Caveolin-1 and -2 were significantly increased with MU. The changes observed in gene expression with MU were reversed by gravitational mechanical loading (MU/ML). Conclusions: Gravitational MU decreases inflammatory and adhesion molecule gene expression and these changes are reversed by short periods of ML. This points towards the importance of gravitational loading in ECs function and cellular interactions.

Simvastatin mitigates cytokine induced cell adhesion molecule upregulation in cultured human endothelial cells

2003 ◽  
Vol 114 (2) ◽  
pp. 284
Author(s):  
H. Jain ◽  
E.M. Troop ◽  
A. Rezvan ◽  
M.B. Silber ◽  
A.D. Brooks ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Cell Adhesion ◽  
Adhesion Molecule ◽  
Cell Adhesion Molecule ◽  
Human Endothelial Cells
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