scholarly journals Nanoparticle-mediated endothelial cell-selective delivery of pitavastatin induces functional collateral arteries (therapeutic arteriogenesis) in a rabbit model of chronic hind limb ischemia

2010 ◽  
Vol 52 (2) ◽  
pp. 412-420 ◽  
Author(s):  
Shinichiro Oda ◽  
Ryoji Nagahama ◽  
Kaku Nakano ◽  
Tetsuya Matoba ◽  
Mitsuki Kubo ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Hind Limb ◽  
Rabbit Model ◽  
Limb Ischemia ◽  
Hind Limb Ischemia ◽  
Collateral Arteries ◽  
Selective Delivery

Abstract 17597: Endogenous Del-1 is a Negative Regulator of Ischemia-induced Angiogenesis by Interacting With the Leukocytic LFA-1 Integrin

Circulation ◽  
2014 ◽  
Vol 130 (suppl_2) ◽  
Author(s):  
Sebastian Cremer ◽  
Anne Klotzsche-von Ameln ◽  
Alessia Orlandi ◽  
Irina Korovina ◽  
Bettina Gercken ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Hind Limb ◽  
Hematopoietic Cells ◽  
Limb Ischemia ◽  
Inflammatory Cells ◽  
Capillary Density ◽  
Negative Regulator ◽  
Hind Limb Ischemia ◽  
Autonomous Pathway

Developmental endothelial locus-1 (Del-1) is an endothelial cell-derived secreted protein circulating in blood and associated with the cell surface and the extracellular matrix. As we previously demonstrated, Del-1 restricts leukocyte recruitment by inhibiting the β2-integrin, LFA-1. Leukocytes and progenitor cells (PC) may contribute to angiogenesis. The role of endogenous Del-1 in angiogenesis is elusive. We found, that physiological angiogenesis of the developing retina was not affected in the Del-1-/- mice compared to the wildtype (WT) mice. Surprisingly, Del-1-/- mice displayed a significantly increased angiogenic response compared to WT mice after induction of hind limb ischemia (144 ± 6 % increase of capillary density) and retinal ischemia (retinopathy of prematurity model) suggesting that endogenous Del-1 is an inhibitor of ischemia-induced neovascularization. Silencing of Del-1 with siRNA did not affect the angiogenic sprouting of endothelial cell (EC) spheroids, indicating that Del-1 blocks angiogenesis in a non-endothelial cell autonomous pathway. Soluble Del-1 blocked the adhesion of inflammatory cells on EC monolayers. In line with these results, ischemic muscles and ischemic retinae from Del-1-/- mice displayed an enhanced infiltration with inflammatory cells compared to the WT mice. Since Del-1 blocks inflammatory cell homing by inhibiting the leukocytic LFA-1-integrin, we addressed the role of the Del-1/LFA-1-integrin interaction on the inhibitory function of endogenous Del-1 on angiogenesis. Indeed, Del-1/LFA-1-double deficiency reversed the pro-angiogenic phenotype of the Del-1-/- mice to the level of WT mice in the model of hind limb ischemia. Thus, the inhibitory role of Del-1 on neovascularization is mediated by the interaction of Del-1 with the LFA-1-integrin. Moreover, Del-1-deficiency led to an increased homing of intravenously injected murine fluorescence-labeled WT Lin- BM PC in ischemic muscles in comparison to WT mice after the induction of hind limb ischemia. Taken together, Del-1 acts as a negative regulator of ischemia-induced angiogenesis by interacting with the LFA-1-integrin expressed in hematopoietic cells, thereby inhibiting the homing of hematopoietic cells to ischemic tissues.

scholarly journals Enhanced angiogenesis by gelatin hydrogels incorporating basic fibroblast growth factor in rabbit model of hind limb ischemia

2007 ◽  
Vol 22 (2) ◽  
pp. 104-108 ◽  
Author(s):  
Kazuhiko Doi ◽  
Tadashi Ikeda ◽  
Akira Marui ◽  
Toshihiro Kushibiki ◽  
Yoshio Arai ◽  
...  
Keyword(s):  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Basic Fibroblast Growth Factor ◽  
Hind Limb ◽  
Rabbit Model ◽  
Limb Ischemia ◽  
Fibroblast Growth ◽  
Hind Limb Ischemia

Ex vivo gene delivery of ephrin-B2 induces development of functional collateral vessels in a rabbit model of hind limb ischemia

2009 ◽  
Vol 49 (1) ◽  
pp. 192-198 ◽  
Author(s):  
Masatake Katsu ◽  
Hiroyuki Koyama ◽  
Hiromitsu Maekawa ◽  
Hiroki Kurihara ◽  
Hiroaki Uchida ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Hind Limb ◽  
Ex Vivo ◽  
Rabbit Model ◽  
Limb Ischemia ◽  
Hind Limb Ischemia ◽  
Collateral Vessels

scholarly journals Adrenomedullin Gene Transfer Induces Therapeutic Angiogenesis in a Rabbit Model of Chronic Hind Limb Ischemia

Circulation ◽  
2004 ◽  
Vol 109 (4) ◽  
pp. 526-531 ◽  
Author(s):  
Noriyuki Tokunaga ◽  
Noritoshi Nagaya ◽  
Mikiyasu Shirai ◽  
Etsuro Tanaka ◽  
Hatsue Ishibashi-Ueda ◽  
...  
Keyword(s):  
Gene Transfer ◽  
Hind Limb ◽  
Rabbit Model ◽  
Limb Ischemia ◽  
Therapeutic Angiogenesis ◽  
Hind Limb Ischemia

The effects of dichloroacetate in a rabbit model of acute hind-limb ischemia and reperfusion

2003 ◽  
Vol 197 (4) ◽  
pp. 591-595 ◽  
Author(s):  
Jeffrey S Wilson ◽  
Greg Rushing ◽  
Brad L Johnson ◽  
Jeffrey A Kline ◽  
Jaime L Parker ◽  
...  
Keyword(s):  
Hind Limb ◽  
Rabbit Model ◽  
Limb Ischemia ◽  
Ischemia And Reperfusion ◽  
Hind Limb Ischemia

Hind Limb Ischemia in Rabbit Model: T2-prepared versus Time-of-Flight MR Angiography at 3 T

Radiology ◽  
2007 ◽  
Vol 245 (3) ◽  
pp. 761-769 ◽  
Author(s):  
Grigorios Korosoglou ◽  
Wesley D. Gilson ◽  
Michael Schär ◽  
Ali Ustun ◽  
Lawrence V. Hofmann ◽  
...  
Keyword(s):  
Mr Angiography ◽  
Hind Limb ◽  
Rabbit Model ◽  
Time Of Flight ◽  
Limb Ischemia ◽  
Hind Limb Ischemia

Vascular Regeneration by Repeated Infusions of Basic Fibroblast Growth Factor in a Rabbit Model of Hind-Limb Ischemia

2009 ◽  
Vol 192 (6) ◽  
pp. W306-W310 ◽  
Author(s):  
Ayumi Seko ◽  
Norihisa Nitta ◽  
Akinaga Sonoda ◽  
Shinichi Ohta ◽  
Masashi Takahashi ◽  
...  
Keyword(s):  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Basic Fibroblast Growth Factor ◽  
Hind Limb ◽  
Rabbit Model ◽  
Limb Ischemia ◽  
Fibroblast Growth ◽  
Hind Limb Ischemia ◽  
Vascular Regeneration

scholarly journals MR Angiography of Collateral Arteries in a Hind Limb Ischemia Model: Comparison between Blood Pool Agent Gadomer and Small Contrast Agent Gd-DTPA

PLoS ONE ◽  
2011 ◽  
Vol 6 (1) ◽  
pp. e16159 ◽  
Author(s):  
Karolien Jaspers ◽  
Bas Versluis ◽  
Tim Leiner ◽  
Petra Dijkstra ◽  
Marlies Oostendorp ◽  
...  
Keyword(s):  
Contrast Agent ◽  
Mr Angiography ◽  
Hind Limb ◽  
Model Comparison ◽  
Limb Ischemia ◽  
Blood Pool ◽  
Blood Pool Agent ◽  
Hind Limb Ischemia ◽  
Collateral Arteries
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