Surgery-Derived Reactive Oxygen Species Produced by Polymorphonuclear Leukocytes Promote Tumor Recurrence: Studies in an In Vitro Model

2007 ◽  
Vol 140 (1) ◽  
pp. 115-120 ◽  
Author(s):  
Wilhelmina M.U. van Grevenstein ◽  
Arend G.J. Aalbers ◽  
Sander ten Raa ◽  
Wim Sluiter ◽  
Leo J. Hofland ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Polymorphonuclear Leukocytes ◽  
Tumor Recurrence ◽  
In Vitro Model ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Vitro Model

scholarly journals Effects of uremic solutes on reactive oxygen species in vitro model systems as a possibility of support the renal function management

2015 ◽  
Vol 16 (1) ◽  
Author(s):  
Renata P Assis ◽  
Juliana FA Castro ◽  
Vânia O Gutierres ◽  
Carlos A Arcaro ◽  
Renata S Brotto ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Renal Function ◽  
In Vitro Model ◽  
Reactive Oxygen ◽  
Model Systems ◽  
Oxygen Species ◽  
Uremic Solutes ◽  
Function Management ◽  
Vitro Model

Sevoflurane Protects Rat Mixed Cerebrocortical Neuronal–Glial Cell Cultures against Transient Oxygen–Glucose Deprivation

2006 ◽  
Vol 105 (5) ◽  
pp. 990-998 ◽  
Author(s):  
Paula T. Canas ◽  
Lionel J. Velly ◽  
Christelle N. Labrande ◽  
Benjamin A. Guillet ◽  
Valérie Sautou-Miranda ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
In Vitro Model ◽  
Glutamate Uptake ◽  
Reactive Oxygen ◽  
Glucose Deprivation ◽  
Oxygen Species ◽  
Lactate Dehydrogenase Release ◽  
Vitro Model ◽  
Glial Cell Cultures

Background The purpose of this study was to clarify the role of glutamate and reactive oxygen species in sevoflurane-mediated neuroprotection on an in vitro model of ischemia-reoxygenation. Methods Mature mixed cerebrocortical neuronal-glial cell cultures, treated or not with increasing concentrations of sevoflurane, were exposed to 90 min combined oxygen-glucose deprivation (OGD) in an anaerobic chamber followed by reoxygenation. Cell death was quantified by lactate dehydrogenase release into the media and cell viability by reduction of 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium by mitochondrial succinate dehydrogenase. Extracellular concentrations of glutamate and glutamate uptake were assessed at the end of the ischemic injury by high-performance liquid chromatography and incorporation of L-[H]glutamate into cells, respectively. Free radical generation in cells was assessed 6 h after OGD during the reoxygenation period using 2',7'-dichlorofluorescin diacetate, which reacts with intracellular radicals to be converted to its fluorescent product, 2',7'-dichlorofluorescin, in cell cytosol. Results Twenty-four hours after OGD, sevoflurane, in a concentration-dependent manner, significantly reduced lactate dehydrogenase release and increased cell viability. At the end of OGD, sevoflurane was able to reduce the OGD-induced decrease in glutamate uptake. This effect was impaired in the presence of threo-3-methyl glutamate, a specific inhibitor of the glial transporter GLT1. Sevoflurane counteracted the increase in extracellular level of glutamate during OGD and the generation of reactive oxygen species during reoxygenation. Conclusion Sevoflurane had a neuroprotective effect in this in vitro model of ischemia-reoxygenation. This beneficial effect may be explained, at least in part, by sevoflurane-induced antiexcitotoxic properties during OGD, probably depending on GLT1, and by sevoflurane-induced decrease of reactive oxygen species generation during reoxygenation.

The Influence of Some Immunosuppressive Drugs on the Metabolic Activity of Human Phagocytes and Lymphocytes in vitro

1998 ◽  
Vol 11 (3) ◽  
pp. 155-162 ◽  
Author(s):  
M. Dušková ◽  
L. Dušek ◽  
M. Čìž ◽  
A. Lojek ◽  
H. Slavíková
Keyword(s):  
Reactive Oxygen Species ◽  
Cyclosporine A ◽  
Polymorphonuclear Leukocytes ◽  
Thymidine Incorporation ◽  
Reactive Oxygen ◽  
Blast Transformation ◽  
Oxygen Species ◽  
Fk 506 ◽  
Control Value

The effect of azathioprine, cyclosporine A and FK 506 on the production of reactive oxygen species by polymorphonuclear leukocytes (Iuminol-dependent chemiluminescence) and on the blast transformation of lymphocytes ([3H]thymidine incorporation) was studied in dose-response experiments under in vitro conditions. Although there were no significant effects of immunosuppressives on non-stimulated blast transformation, FK 506 and cyclosporine A significantly inhibited the blast transformation stimulated by concanavaline A and protein A and the effects made it possible to build 2nd-order polynomial dose-response models. Azathioprine was found to be a relatively weak inhibitor of [3H]thymidine incorporation in lymphocytes (76% of control value). Spontaneous production of reactive oxygen species by polymorphonuclear leukocytes was significantly inhibited, particularly by FK 506 (1–100 ng.ml−1) in comparison to the control value, while there was no effect of the immunosuppressives on this system activated either by starch grains or zymosan. Only the highest applied concentrations of azathioprine (100 ng.ml−1) and cyclosporine A (1000 ng.ml−1) led to a significant decline in spontaneous phagocytosis. The direct effect of immunosuppressives on activated production of reactive oxygen species by neutrophiles was not proved.

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