scholarly journals Development and validation of bioanalytical UHPLC-UV method for simultaneous analysis of unchanged fenofibrate and its metabolite fenofibric acid in rat plasma: Application to pharmacokinetics

2017 ◽  
Vol 25 (1) ◽  
pp. 128-135 ◽  
Author(s):  
Rayan G. Alamri ◽  
Kazi Mohsin ◽  
Ajaz Ahmad ◽  
Mohammad Raish ◽  
Fars K. Alanazi
Keyword(s):  
Rat Plasma ◽  
Simultaneous Analysis ◽  
Fenofibric Acid ◽  
Development And Validation

Development and Validation of an LC-MS/MS Method for Simultaneous Determination of Canagliflozin and Metformin HCl in Rat Plasma and its Application

2020 ◽  
Vol 16 (6) ◽  
pp. 752-762
Author(s):  
Vivek Nalawade ◽  
Vaibhav A. Dixit ◽  
Amisha Vora ◽  
Himashu Zade
Keyword(s):  
Internal Standard ◽  
Rat Plasma ◽  
Precipitation Method ◽  
Herbal Extracts ◽  
One Step ◽  
Precision And Accuracy ◽  
Development And Validation ◽  
The Impact ◽  
Metformin Hcl

Background: Food and herbal extracts rich in Quercetin (QRT) are often self-medicated by diabetics and can potentially alter the pharmacokinetics (PK) of Metformin HCl (MET) and Canagliflozin (CNG) leading to food or herb-drug interactions and reduced therapeutic efficacy. However, the impact of these flavonoids on the pharmacokinetic behaviour of MET and CNG is mostly unknown. Methods: A simple one-step protein precipitation method was developed for the determination of MET and CNG from rat plasma. The mobile phase chosen was MeOH 65% and 35% water containing 0.1% formic acid at a flow rate of 1mL/min. Results: The retention time of MET, internal standard (Valsartan) and CNG was 1.83, 6.2 and 8.2 min, respectively. The method was found to be linear in the range of 200 - 8000 ng/mL for CNG and 100 = 4000 ng/ml for MET. Precision and accuracy of the method were below 20% at LLOQ and below 15% for LQC, MQC, and HQC. Conclusion: The method was successfully applied for the determination of PK of MET and CNG by using 100 μL of rat plasma. QRT co-administration affects the PK parameters of MET and CNG. This alteration in PK parameters might be of significant use for clinicians and patients.

Development and validation of a HPLC method for determination of levonorgestrel and quinestrol in rat plasma

2009 ◽  
Vol 24 (7) ◽  
pp. 706-710 ◽  
Author(s):  
Tao Tang ◽  
Pingliang Li ◽  
Laixin Luo ◽  
Dazhao Shi ◽  
Jianqiang Li ◽  
...  
Keyword(s):  
Rat Plasma ◽  
Hplc Method ◽  
Development And Validation

scholarly journals Development and validation of HPLC methods for simultaneous analysis of 6 antiretrovirals in pharmaceutical formulations

2019 ◽  
Vol 8 (6) ◽  
pp. 200-207
Author(s):  
Mankulu Kakumba Jocelyn ◽  
Muhindo Mahamba Augustin ◽  
Mbenza Puati Adelard ◽  
Mbinze Kindenge Jeremie ◽  
Kimbeni Malongo Tresor
Keyword(s):  
Pharmaceutical Formulations ◽  
Simultaneous Analysis ◽  
Development And Validation
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