Diagnosis of Liposcelis entomophila (Insecta: Psocodea: Liposcelididae) based on morphological characteristics and DNA barcodes

2012 ◽  
Vol 48 ◽  
pp. 120-125 ◽  
Author(s):  
Qianqian Yang ◽  
Zuzana Kučerová ◽  
Zhihong Li ◽  
Irma Kalinović ◽  
Václav Stejskal ◽  
...  
Keyword(s):  
Morphological Characteristics ◽  
Dna Barcodes ◽  
Liposcelis Entomophila

scholarly journals A Systematic Study on DNA Barcoding of Medicinally Important Genus Epimedium L. (Berberidaceae)

Genes ◽  
2018 ◽  
Vol 9 (12) ◽  
pp. 637 ◽  
Author(s):  
Mengyue Guo ◽  
Yanqin Xu ◽  
Li Ren ◽  
Shunzhi He ◽  
and Xiaohui Pang
Keyword(s):  
Dna Barcode ◽  
Morphological Characteristics ◽  
Identification Accuracy ◽  
Dna Barcodes ◽  
Success Rates ◽  
Data Set ◽  
Herba Epimedii ◽  
Potential Risks ◽  
Medicinal Properties ◽  
Species Being

Genus Epimedium consists of approximately 50 species in China, and more than half of them possess medicinal properties. The high similarity of species’ morphological characteristics complicates the identification accuracy, leading to potential risks in herbal efficacy and medical safety. In this study, we tested the applicability of four single loci, namely, rbcL, psbA-trnH, internal transcribed spacer (ITS), and ITS2, and their combinations as DNA barcodes to identify 37 Epimedium species on the basis of the analyses, including the success rates of PCR amplifications and sequencing, specific genetic divergence, distance-based method, and character-based method. Among them, character-based method showed the best applicability for identifying Epimedium species. As for the DNA barcodes, psbA-trnH showed the best performance among the four single loci with nine species being correctly differentiated. Moreover, psbA-trnH + ITS and psbA-trnH + ITS + rbcL exhibited the highest identification ability among all the multilocus combinations, and 17 species, of which 12 are medicinally used, could be efficiently discriminated. The DNA barcode data set developed in our study contributes valuable information to Chinese resources of Epimedium. It provides a new means for discrimination of the species within this medicinally important genus, thus guaranteeing correct and safe usage of Herba Epimedii.

scholarly journals DNA barcodes and morphology of P. krempfi, P. mekongensis, and P. elongatus

2016 ◽  
Vol 14 (1) ◽  
pp. 29-37 ◽  
Author(s):  
Dương Thúy Yên ◽  
Nguyễn Kiệt ◽  
Bùi Sơn Nên ◽  
Nguyễn Văn Thường ◽  
Nguyễn Bạch Loan ◽  
...  
Keyword(s):  
Dna Barcode ◽  
Morphological Characteristics ◽  
Mekong Delta ◽  
Genetic Distances ◽  
Dna Barcodes ◽  
Gill Rakers ◽  
Coi Sequences ◽  
Relationship Of ◽  
Metric Traits ◽  
I Gene

Three Pangasius species including P. krempfi, P. elongatus and P. mekongensis, are economically important. They can be mis-identified due to similar external appreance at small sizes. This study aimed to distinguish these species based on their differences in DNA barcode, COI (cytochrome c oxidase subunit I) gene, and morphological characteristics. Fish with various sizes (>90 samples/species) were sampled at the lower Mekong delta region. Kimura-2 parameter genetic distances based on COI sequences of three species (15 samples, in which, 4 unique sequences were assigned Genbank accession numbers from KT289877 to KT289880) are relatively high, ranging 9.33 – 12.10 %. Morphological measurements show that coutanble traits including numbers of fin rays and the first gill rakers vary in similar ranges but ratios of metric traits are significantly different among three species (P<0.01). Principle component analysis using metric traits sets three species apart. P. elongatus is characterized by elongated body, long caudal preduncle, large eyes, and retangle palatine tooth plates. P. krempfi differs from P. mekongesis in characteristics on their head. The number of sections, shape and length of barbel are different among three species. Phylogenetic relationship of three species based on morphology and COI sequences indicate that P. krempfi is closer to P. mekongenis rather than P. elongatus, and that the distance between P. mekongenis and P. elongatus is the largest.

scholarly journals Revision of Claraeola (Diptera, Pipunculidae) in the Middle East based on morphology and DNA barcodes

ZooKeys ◽  
2019 ◽  
Vol 873 ◽  
pp. 85-111 ◽  
Author(s):  
Behnam Motamedinia ◽  
Jeffrey H. Skevington ◽  
Scott Kelso
Keyword(s):  
Middle East ◽  
Sequence Data ◽  
Morphological Characteristics ◽  
Identification Key ◽  
Dna Barcodes ◽  
Mitochondrial Coi ◽  
Coi Barcoding

The Middle East species of Claraeola Aczél (Diptera, Pipunculidae) are revised based on morphological characteristics and sequence data from the mitochondrial COI barcoding gene, using a novel COI mini-barcode protocol. Four new Claraeola species are described: C. bousynterga Motamedinia &amp; Skevington, sp. nov., C. heidiae Motamedinia &amp; Skevington, sp. nov., C. khuzestanensis Motamedinia &amp; Skevington, sp. nov., and C. mantisphalliga Motamedinia &amp; Skevington, sp. nov.Eudorylas thekkadiensis Kapoor, Grewal &amp; Sharma, 1987 is transferred to Claraeola, C. thekkadiensis (comb. nov.). Diagnoses, illustrations, an identification key, and a distributional map are given for the Middle East species.

Description of larva and female of Polypedilum (Probolum) bullum Zhang & Wang with DNA barcodes

Zootaxa ◽  
2020 ◽  
Vol 4890 (2) ◽  
pp. 257-265
Author(s):  
QI LEI ◽  
JINXIAN CHEN ◽  
CHAO SONG ◽  
XIN QI ◽  
RUILEI ZHANG
Keyword(s):  
Morphological Characteristics ◽  
Dna Barcodes ◽  
First Time

The larva and female of Polypedilum (Probolum) bullum Zhang & Wang associated with morphological characteristics and DNA barcodes are described and illustrated for the first time. The female is characteristic with developed dorsomesal lobe and ventrolateral lobe both densely covered with apical setae, ventrolateral lobe partially covered by dorsomesal lobe. The larva is distinguished by the shape of mentum, pecten epipharyngis, labral SI and labral SII. 

scholarly journals Identifying pelagic fish eggs in the southeast Yucatan Peninsula using DNA barcodes

Genome ◽  
2016 ◽  
Vol 59 (12) ◽  
pp. 1117-1129 ◽  
Author(s):  
E. Leyva-Cruz ◽  
L. Vásquez-Yeomans ◽  
L. Carrillo ◽  
M. Valdez-Moreno
Keyword(s):  
Morphological Characteristics ◽  
Dna Barcodes ◽  
Katsuwonus Pelamis ◽  
Istiophorus Platypterus ◽  
Coryphaena Hippurus ◽  
New Information ◽  
Mesoamerican Barrier Reef System ◽  
Mexican Caribbean ◽  
Crevalle Jack ◽  
Kajikia Albida

In the waters surrounding Banco Chinchorro in the Mexican Caribbean are spawning and nursery areas for many types of fish. In this natural environment, as opposed to under controlled laboratory conditions, it is almost impossible to link an individual egg to the adult that laid it. This makes identifying the species of the eggs difficult. However, DNA barcodes have made this easier. In the present study, 300 eggs were processed for molecular analysis, from which 139 sequences were obtained. We identified 42 taxa (33 species with their binomial names), 35 genera, and 24 families. The identified eggs included those from Ariomma melanum, which is the first recording of this species in the Mexican Caribbean. Eggs from economically important fish species were also identified, including frigate tuna (Auxis thazard), crevalle jack (Caranx hippos), common dolphinfish (Coryphaena hippurus), sailfish (Istiophorus platypterus), white marlin (Kajikia albida), skipjack tuna (Katsuwonus pelamis), blackfin tuna (Thunnus atlanticus), and swordfish (Xiphias gladius). We have also described new morphological characteristics and captured photographs for 21 species, as well as obtained new information about spawning locality and time for 16 species. This valuable information will provide the basis to develop more effective conservation measures for sustainable fisheries and protection of the Mesoamerican Barrier Reef System.

scholarly journals DNA barcoding reveals a species group of the genus Campiglossa (Diptera, Tephritidae, Tephritinae) with recognition of a new species from East Asia and previously unknown females of Campiglossa coei (Hardy)

ZooKeys ◽  
2019 ◽  
Vol 899 ◽  
pp. 1-36 ◽  
Author(s):  
Ho-Yeon Han ◽  
Kyung-Eui Ro
Keyword(s):  
Structural Modification ◽  
Dna Analysis ◽  
Morphological Characteristics ◽  
Species Group ◽  
Full Description ◽  
Dna Barcodes ◽  
Monophyletic Clade ◽  
Accurate Identification ◽  
Tephritid Species ◽  
A New Species

While analyzing DNA barcodes of all the Korean and some East Asian tephritid species in conjunction with the barcode sequences available from BOLD Systems (www.boldsystems.org), the large and taxonomically enigmatic genus Campiglossa was recovered as a monophyletic clade, together with the genera Dioxyna and Homoeotricha, which are here synonymized for that reason. Ten major lineages are also recognized within the Campiglossa clade: producta group, loewiana group, sororcula group, irrorata group, achyrophori group, difficilis group, luxorientis group, magniceps group, arisanica group, and misella group. Here, more detailed taxonomic accounts are provided for the misella group, including four DNA analysis-recovered members: C. coei, C. misella, C. paramelaenasp. nov., and C. melaena. A single morphological synapomorphy is proposed for this species group: the presence of a large mid-anterior dark wing marking in males with associated structural modification (more apically positioned crossvein R-M than in females). Based on the morphological characteristics, two presumptive members that are only known from male specimens are further recognized: C. pishanica and C. propria from China. A full description of C. paramelaenasp. nov., and a redescription of C. coei, for which only males were previously known, are provided. For all the included species, a taxonomic key, diagnoses, and photographs to aid their accurate identification are given. Finally, C. favillacea is synonymized with C. coei and C. roscida with C. misella, and C. coei and C. pishanica resurrected from the synonymy of C. misella.

scholarly journals Molecular identification of Gracilaria species (Gracilariales, Rhodophyta) obtained from the South Coast of Java Island, Indonesia

2021 ◽  
Vol 22 (7) ◽  
Author(s):  
Maria Dyah Nur Meinita ◽  
Nashiatul Akromah ◽  
Nuraina Andriyani ◽  
Setijanto SETIJANTO ◽  
Dicky Harwanto ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Molecular Identification ◽  
Molecular Diversity ◽  
Morphological Characteristics ◽  
South Coast ◽  
Intraspecific Diversity ◽  
Cox1 Gene ◽  
Dna Barcodes ◽  
The South ◽  
Interspecific Variations

Abstract. Meinita MDN, Akromah N, Andriyani N, Setijanto, Harwanto D, Liu T. 2021. Molecular identification of Gracilaria species (Gracilariales, Rhodophyta) obtained from the South Coast of Java Island, Indonesia. Biodiversitas 22: 3046-3056. The study of seaweeds diversity and species identification is an important component of marine resource management. However, seaweed identification based on morphological characteristics has several limitations. Besides, DNA barcodes or the partial sequences of cytochrome c oxidase I (COX1) have been proved to identify seaweeds at the species level. To date, Gracilaria species molecular diversity has not been studied in the South Coast of Java Island. Hence, this study aimed to identify the Gracilaria spp. obtained from 6 different beaches along the South Coast of Java, based on the COX1 gene. COX1 gene utilization for identifying and observing the genetic diversity, both intraspecific (genetic variation within species) and interspecific (variations between species) of Gracilaria species in this study has produced good results. A total of 13 seaweed samples collected from beaches in this study were identified as Gracilaria salicornia, G. edulis, G. firma, and G. textorii. The results of genetic diversity analysis conducted using the COX1 gene showed the intraspecific diversity of G. edulis obtained from the beaches of Kondang Merak, Kukup, Nusakambangan, and Karapyak was included in the moderate diversity category. Also, the intraspecific diversity of G. salicornia obtained from the beaches of Kondang Merak, Kukup, and Nusakambangan was included in the moderate diversity category, while the intraspecific diversity of G. textorii from Menganti and Karapyak Beach had no diversity.

scholarly journals Three cryptic Anaplecta (Blattodea, Blattoidea, Anaplectidae) species revealed by female genitalia, plus seven new species from China

ZooKeys ◽  
2022 ◽  
Vol 1080 ◽  
pp. 53-97
Author(s):  
Jing Zhu ◽  
Jiawei Zhang ◽  
Xinxing Luo ◽  
Zongqing Wang ◽  
Yanli Che
Keyword(s):  
New Species ◽  
Cryptic Species ◽  
Morphological Characteristics ◽  
The Other ◽  
Female Genitalia ◽  
Dna Barcodes ◽  
Male And Female

Morphological characteristics, including male and female genitalia, combined with DNA barcodes were used to identify 470 Anaplecta specimens sampled from China. Ten Anaplecta species are new to science, including three cryptic species: A. paraomei Zhu &amp; Che, sp. nov., A. condensa Zhu &amp; Che, sp. nov., and A. longihamata Zhu &amp; Che, sp. nov., which are distinguished mainly by their female genitalia. The other seven new species are as follows: A. bicruris Zhu &amp; Che, sp. nov., A. spinosa Zhu &amp; Che, sp. nov., A. ungulata Zhu &amp; Che, sp. nov., A. anomala Zhu &amp; Che, sp. nov., A. serrata Zhu &amp; Che, sp. nov., A. bombycina Zhu &amp; Che, sp. nov., and A. truncatula Zhu &amp; Che, sp. nov. This study illustrates that differences in female genitalia can be used to distinguish among species of Anaplecta. The female genitalia of 19 Chinese Anaplecta species are described and illustrated in this paper.

scholarly journals Efficiency of ITS1-5.8S-ITS2 region in identifying Cordyceps species

2020 ◽  
Vol 16 (4) ◽  
pp. 705-712
Author(s):  
Le Thi Thu Hien ◽  
Ha Hong Hanh
Keyword(s):  
Dna Sequences ◽  
Phylogenetic Trees ◽  
Nearest Neighbor ◽  
Dna Barcode ◽  
Morphological Characteristics ◽  
Pcr Amplification ◽  
Cordyceps Sinensis ◽  
Its2 Region ◽  
Dna Barcodes ◽  
Plastid Genomes

Cordyceps genus is a well-known traditional medicine worldwide. It contains abundant physiological active compounds that were demonstrated to perform benefit in reducing progression of cancer as well as protecting human health. Accurately classifying species in this genus is essential in order to prevent commercial counterfeit medicines. Nowadays, a taxonomic classification of species based on DNA sequences can overcome the existed limitation in identifying by using only morphological characteristics of this genus. DNA barcodes are standard short genomic regions that are universally present in target lineages and has sufficient sequence variation to discriminate species in the genus. A variety of loci has been suggested as DNA barcodes for plants, including genes and non-coding regions in the nuclear and plastid genomes such as psbA-trnH, matK, rbcL, and ITS. Thus, the objective of this study was to identify selected species of Cordyceps genus using DNA barcodes. Seven strains of Cordyceps were collected. Total DNA extraction and purification, PCR amplification and DNA sequencing were performed with standard chemicals and kits. The candidate ITS1-5.8S-ITS2 region was amplified and sequenced. Data were analyzed using Bioedit 7.2.6 and MEGA 7 softwares. Analysis of seven obtained DNA barcode sequences of collected samples revealed that the ITS1-5.8S-ITS2 region provided high species discriminating power for Cordyceps genus. Accordingly, phylogenetic trees based on this DNA barcode exhibited six samples had closed relationship to Cordyceps militaris, while another specimen was the nearest neighbor to Cordyceps sinensis with average similarities at 99.82% and 99.81%, respectively. Our results support the identification of valuable medicinal plant species within Cordyceps genus.

scholarly journals Morphological characteristics of Talinum paniculatum, and nucleotide sequences of ITS region, rpoC1 and rpoB genes

2018 ◽  
Vol 16 (3) ◽  
pp. 451-458
Author(s):  
Vu Thi Nhu Trang ◽  
Ho Manh Tuong ◽  
Le Van Son ◽  
Nguyen Thi Tam ◽  
Chu Hoang Mau
Keyword(s):  
Morphological Analysis ◽  
Morphological Characteristics ◽  
Its Region ◽  
Nucleotide Sequences ◽  
Family Characteristics ◽  
Skin Infections ◽  
Dna Barcodes ◽  
Ripe Fruit ◽  
Tuberous Roots ◽  
Small Roots

Jewels of Opar (T. paniculatum) belongs to Talinum genus, Portulacaceae family which contains secondary metabolites such as phytosterols, saponins, flavonoids, tannins, steroids. These organic compounds have anti-viral effects and are very effective on Herpes’ disease and skin infections. Besides, Jewels of Opar can also be used as a supporting medicine for Parkinson’s disease, heart disease and for lowering blood cholesterols. Currently, the identification of T. paniculatum has been mainly based on morphological analysis. However, this method often encounters obstacles when T. paniculatum has been completely or partially processed. In this work, we present the results of morphological characteristics, taxonomy and sequences characterisation of ITS region and rpoC1, rpoB genes of T. paniculatum in Northern provinces of Vietnam. Tuberous roots of T. paniculatum are cylindrical with many small roots. The stems are upright and divided into several branches. The stems are upright and divided into several branches. The leaves are staggered, generally oval, ovate-oblong, or egg back shaped; thick, glossy with wavy veins, without hairs. The flowers of the plants have five reddish purple wings, two sepals, more than ten stamens, and a spherical ovary. The fruits are small, and the ripe fruit is ash gray in color. The seeds are very small, slightly flat, and black. Internal transcribed spacer (ITS) region and two partial sequences of rpoC1 and rpoB genes isolated from T. paniculatum plants are 643 bp, 595 bp and 518 bp in length, respectively. Based on the combination of the characteristics of morphology and nucleotide sequences of ITS region, rpoC1 and rpoB genes, the Jewels of Opar samples collected in some northern provinces of Vietnam were determined to belong to T. paniculatum species, Talinum genus, Portulacaceae family. Characteristics of sequences of ITS region and rpoC1, rpoB genes are valuable for exploiting DNA barcodes to identify T. paniculatum in Vietnam.

Sign in / Sign up

Export Citation Format

Share Document