Detection of Tribolium castaneum (Herbst) volatile defensive secretions by solid phase microextraction–capillary gas chromatography (SPME-CGC)

2007 ◽  
Vol 43 (4) ◽  
pp. 540-545 ◽  
Author(s):  
M. Luciana Villaverde ◽  
M. Patricia Juárez ◽  
Sergio Mijailovsky
Keyword(s):  
Gas Chromatography ◽  
Tribolium Castaneum ◽  
Capillary Gas Chromatography ◽  
Solid Phase Microextraction ◽  
Solid Phase ◽  
Defensive Secretions

Use of solid phase microextraction coupled to capillary gas chromatography-mass spectrometry for screening Fusarium spp. based on their volatile sesquiterpenes

2010 ◽  
Vol 3 (2) ◽  
pp. 121-128 ◽  
Author(s):  
J. Girotti ◽  
I. Malbrán ◽  
G. Lori ◽  
M. Juárez
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Capillary Gas Chromatography ◽  
Solid Phase Microextraction ◽  
Solid Phase ◽  
Fusarium Species ◽  
Gas Chromatography Mass Spectrometry ◽  
Head Blight ◽  
Pampa Region ◽  
Blight Disease

Solid phase microextraction (SPME) coupled to capillary gas chromatography (CGC) and mass spectrometry (MS) was used to evaluate the use of fungal volatiles to discriminate Fusarium species from wheat cultivars in the Argentina pampa region. Monosporic fungal isolates were grown on rice in sealed containers for 1 week and volatile organic compounds (VOC) were sampled for 30 min from the head space by SPME and analysed by CGC and CGCMS. VOC profiles of Fusarium species F. graminearum, F. poae, F. equiseti, F. verticillioides and F. oxysporum were discriminated by comparison of their profiles in the elution zone corresponding to sesquiterpenes. Trichothecene-producer and non-trichothecene producer Fusarium species were separated by the presence of trichodiene in their VOC fingerprints. Within trichothecene-producers, F. graminearum, F. poae and F. equiseti differed on the structure of their volatile sesquiterpenes. This technique might be also helpful to detect F. graminearum, the major head blight disease-producing fungus in the region.

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