scholarly journals Multiple enhancer regions located at significant distances upstream of the transcriptional start site mediate RANKL gene expression in response to 1,25-dihydroxyvitamin D3

2007 ◽  
Vol 103 (3-5) ◽  
pp. 430-434 ◽  
Author(s):  
Sungtae Kim ◽  
Miwa Yamazaki ◽  
Lee A. Zella ◽  
Mark B. Meyer ◽  
Jackie A. Fretz ◽  
...  
Keyword(s):  
Gene Expression ◽  
Transcriptional Start Site ◽  
Start Site ◽  
Dihydroxyvitamin D3 ◽  
1,25 Dihydroxyvitamin D3

C-JUN N-TERMINAL KINASE MODULATES 1,25-DIHYDROXYVITAMIN D3-INDUCED CYTOCHROME P450 3A4 GENE EXPRESSION

2004 ◽  
Vol 32 (7) ◽  
pp. 685-688 ◽  
Author(s):  
Yoko Yasunami ◽  
Hirokazu Hara ◽  
Tatsunori Iwamura ◽  
Tadashi Kataoka ◽  
Tetsuo Adachi
Keyword(s):  
Gene Expression ◽  
Cytochrome P450 ◽  
Cytochrome P450 3A4 ◽  
Dihydroxyvitamin D3 ◽  
1,25 Dihydroxyvitamin D3

scholarly journals Transcriptional Regulation ofFucosyltransferase 1Gene Expression in Colon Cancer Cells

2013 ◽  
Vol 2013 ◽  
pp. 1-9 ◽  
Author(s):  
Fumiko Taniuchi ◽  
Koji Higai ◽  
Tomomi Tanaka ◽  
Yutaro Azuma ◽  
Kojiro Matsumoto
Keyword(s):  
Gene Expression ◽  
Colon Cancer ◽  
Transcriptional Regulation ◽  
Binding Site ◽  
Transcriptional Start Site ◽  
Dominant Negative ◽  
Site Directed Mutagenesis ◽  
Luciferase Assay ◽  
Start Site ◽  
Lewis Y

Theα1,2-fucosyltransferase I (FUT1) enzyme is important for the biosynthesis of H antigens, Lewis B, and Lewis Y. In this study, we clarified the transcriptional regulation of FUT1 in the DLD-1 colon cancer cell line, which has high expression of Lewis B and Lewis Y antigens, expresses theFUT1gene, and showsα1,2-fucosyltransferase (FUT) activity. 5′-rapid amplification of cDNA ends revealed a FUT1 transcriptional start site −10 nucleotides upstream of the site registered at NM_000148 in the DataBase of Human Transcription Start Sites (DBTSS). Using the dual luciferase assay,FUT1gene expression was shown to be regulated at the region −91 to −81 nt to the transcriptional start site, which contains the Elk-1 binding site. Site-directed mutagenesis of this region revealed the Elk-1 binding site to be essential for FUT1 transcription. Furthermore, transfection of the dominant negative Elk-1 gene, and the chromatin immunoprecipitation (CHIp) assay, supported Elk-1-dependent transcriptional regulation ofFUT1gene expression in DLD-1 cells. These results suggest that a defined region in the 5′-flanking region of FUT1 is critical for FUT1 transcription and that constitutive gene expression ofFUT1is regulated by Elk-1 in DLD-1 cells.

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