Phosphorylation and function of the hamster adrenal steroidogenic acute regulatory protein (StAR)

2004 ◽  
Vol 91 (4-5) ◽  
pp. 259-271 ◽  
Author(s):  
Alain Fleury ◽  
Axel P. Mathieu ◽  
Lyne Ducharme ◽  
Dale Buchanan Hales ◽  
Jean-Guy LeHoux
Keyword(s):  
Regulatory Protein ◽  
Steroidogenic Acute Regulatory Protein ◽  
Steroidogenic Acute Regulatory ◽  
And Function

scholarly journals Peripheral-Type Benzodiazepine Receptor-Mediated Action of Steroidogenic Acute Regulatory Protein on Cholesterol Entry into Leydig Cell Mitochondria

2005 ◽  
Vol 19 (2) ◽  
pp. 540-554 ◽  
Author(s):  
Thierry Hauet ◽  
Zhi-Xing Yao ◽  
Himangshu S. Bose ◽  
Christopher T. Wall ◽  
Zeqiu Han ◽  
...  
Keyword(s):  
Regulatory Protein ◽  
Benzodiazepine Receptor ◽  
Steroidogenic Acute Regulatory Protein ◽  
Rate Determining Step ◽  
Human Choriogonadotropin ◽  
Leydig Tumor Cells ◽  
Peripheral Type ◽  
Steroidogenic Acute Regulatory ◽  
And Function

Abstract Hormone-induced steroid biosynthesis begins with the transfer of cholesterol from intracellular stores into mitochondria. Steroidogenic acute regulatory protein (StAR) and peripheral-type benzodiazepine receptor (PBR) have been implicated in this rate-determining step of steroidogenesis. MA-10 mouse Leydig tumor cells were treated with and without oligodeoxynucleotides (ODNs) antisense to PBR and StAR followed by treatment with saturating concentrations of human choriogonadotropin. Treatment with ODNs antisense but not missense for both proteins inhibited the respective protein expression and the ability of the cells to synthesize steroids in response to human choriogonadotropin. Treatment of the cells with either ODNs antisense to PBR or a transducible peptide antagonist to PBR resulted in inhibition of the accumulation of the mature mitochondrial 30-kDa StAR protein, suggesting that the presence of PBR is required for StAR import into mitochondria. Addition of in vitro transcribed/translated 37-kDa StAR or a fusion protein of Tom20 (translocase of outer membrane) and StAR (Tom/StAR) to mitochondria isolated from control cells increased pregnenolone formation. Mitochondria isolated from cells treated with ODNs antisense, but not missense, to PBR failed to form pregnenolone and respond to either StAR or Tom/StAR proteins. Reincorporation of in vitro transcribed/translated PBR, but not PBR missing the cholesterol-binding domain, into MA-10 mitochondria rescued the ability of the mitochondria to form steroids and the ability of the mitochondria to respond to StAR and Tom/StAR proteins. These data suggest that both StAR and PBR proteins are indispensable elements of the steroidogenic machinery and function in a coordinated manner to transfer cholesterol into mitochondria.

Von Hermaphroditos der griechischen Mythologie bis zu DSD der Moderne

Praxis ◽  
2008 ◽  
Vol 98 (1) ◽  
pp. 31-34
Author(s):  
Oestmann ◽  
Mullis ◽  
Stanga
Keyword(s):  
Regulatory Protein ◽  
Steroidogenic Acute Regulatory Protein ◽  
Sich Eine ◽  
Fand Sich ◽  
Steroidogenic Acute Regulatory

Wir berichten über eine heute 34-jährige Frau, die im Alter von 6 Monaten wegen rezidivierendem Erbrechen hospitalisiert werden musste. Als Ursache fand sich eine Nebenniereninsuffizienz mit Verminderung sämtlicher Hormone der Steroidhormonbiosynthese. Die weiteren Abklärungen ergaben bei dem phänotypisch weiblichen Säugling eine lipoide kongenitale adrenale Hyperplasie mit 46,XY DSD. 24 Jahre später konnte in der DNS-Sequenzanalyse ein homozygoter, in der Schweiz vorkommender Basenaustausch des steroidogenic acute regulatory protein-Gens gefunden werden, welcher zu einem Aminosäurenaustausch Leucin 260 Prolin (L260P) führt.

2040-P: Chronic Activation of CREB by Islet Amyloid Increases Star (Steroidogenic Acute Regulatory Protein) Expression in Islets

Diabetes ◽  
2020 ◽  
Vol 69 (Supplement 1) ◽  
pp. 2040-P
Author(s):  
MEGHAN F. HOGAN ◽  
NATHALIE ESSER ◽  
ANDREW T. TEMPLIN ◽  
JOSEPH J. CASTILLO ◽  
SAKENEH ZRAIKA ◽  
...  
Keyword(s):  
Protein Expression ◽  
Regulatory Protein ◽  
Steroidogenic Acute Regulatory Protein ◽  
Islet Amyloid ◽  
Chronic Activation ◽  
Steroidogenic Acute Regulatory
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