Rapid near-atomic resolution single-particle 3D reconstruction with SIMPLE

2018 ◽  
Vol 204 (2) ◽  
pp. 172-181 ◽  
Author(s):  
Cyril F. Reboul ◽  
Simon Kiesewetter ◽  
Michael Eager ◽  
Matthew Belousoff ◽  
Tiangang Cui ◽  
...  
Keyword(s):  
3D Reconstruction ◽  
Single Particle ◽  
Atomic Resolution ◽  
Resolution Single

A3Near-atomic Resolution Single Particle Analysis with the Volta Phase Plate

Microscopy ◽  
2015 ◽  
Vol 64 (suppl 1) ◽  
pp. i9.2-i9
Author(s):  
Radostin Danev ◽  
Maryam Khoshouei ◽  
Wolfgang Baumeister
Keyword(s):  
Single Particle ◽  
Atomic Resolution ◽  
Particle Analysis ◽  
Phase Plate ◽  
Single Particle Analysis ◽  
Resolution Single

scholarly journals Electron counting and beam-induced motion correction enable near-atomic-resolution single-particle cryo-EM

2013 ◽  
Vol 10 (6) ◽  
pp. 584-590 ◽  
Author(s):  
Xueming Li ◽  
Paul Mooney ◽  
Shawn Zheng ◽  
Christopher R Booth ◽  
Michael B Braunfeld ◽  
...  
Keyword(s):  
Single Particle ◽  
Motion Correction ◽  
Atomic Resolution ◽  
Electron Counting ◽  
Induced Motion ◽  
Resolution Single

scholarly journals Implementing Direct Electron Detection Camera K2 and Dose Fractionation for Near Atomic Resolution Single Particle CryoEM

2013 ◽  
Vol 19 (S2) ◽  
pp. 1152-1153 ◽  
Author(s):  
X. Li ◽  
S. Zheng ◽  
D.A. Agard ◽  
Y. Cheng
Keyword(s):  
Single Particle ◽  
Atomic Resolution ◽  
Dose Fractionation ◽  
Electron Detection ◽  
Resolution Single

Extended abstract of a paper presented at Microscopy and Microanalysis 2013 in Indianapolis, Indiana, USA, August 4 – August 8, 2013.

scholarly journals Beam image-shift accelerated data acquisition for near-atomic resolution single-particle cryo-electron tomography

2021 ◽  
Vol 77 (a1) ◽  
pp. a209-a209
Author(s):  
Jonathan Bouvette ◽  
Mario Borgnia ◽  
Hsuan-Fu Liu ◽  
Alberto Bartesaghi ◽  
Roel Schaaper ◽  
...  
Keyword(s):  
Data Acquisition ◽  
Single Particle ◽  
Electron Tomography ◽  
Atomic Resolution ◽  
Beam Image ◽  
Cryo Electron Tomography ◽  
Resolution Single

Structural Studies on the Eukaryotic Ribosome

1990 ◽  
Vol 48 (1) ◽  
pp. 256-257
Author(s):  
Adriana Verschoor ◽  
Ronald Milligan ◽  
Suman Srivastava ◽  
Joachim Frank
Keyword(s):  
Chick Embryo ◽  
3D Reconstruction ◽  
Single Particle ◽  
Mass Density ◽  
Particle Surface ◽  
Uranyl Acetate ◽  
Electron Microscopic ◽  
Eukaryotic Ribosome ◽  
Negative Stain ◽  
Reconstruction Methods

We have studied the eukaryotic ribosome from two vertebrate species (rabbit reticulocyte and chick embryo ribosomes) in several different electron microscopic preparations (Fig. 1a-d), and we have applied image processing methods to two of the types of images. Reticulocyte ribosomes were examined in both negative stain (0.5% uranyl acetate, in a double-carbon preparation) and frozen hydrated preparation as single-particle specimens. In addition, chick embryo ribosomes in tetrameric and crystalline assemblies in frozen hydrated preparation have been examined. 2D averaging, multivariate statistical analysis, and classification methods have been applied to the negatively stained single-particle micrographs and the frozen hydrated tetramer micrographs to obtain statistically well defined projection images of the ribosome (Fig. 2a,c). 3D reconstruction methods, the random conical reconstruction scheme and weighted back projection, were applied to the negative-stain data, and several closely related reconstructions were obtained. The principal 3D reconstruction (Fig. 2b), which has a resolution of 3.7 nm according to the differential phase residual criterion, can be compared to the images of individual ribosomes in a 2D tetramer average (Fig. 2c) at a similar resolution, and a good agreement of the general morphology and of many of the characteristic features is seen.Both data sets show the ribosome in roughly the same ’view’ or orientation, with respect to the adsorptive surface in the electron microscopic preparation, as judged by the agreement in both the projected form and the distribution of characteristic density features. The negative-stain reconstruction reveals details of the ribosome morphology; the 2D frozen-hydrated average provides projection information on the native mass-density distribution within the structure. The 40S subunit appears to have an elongate core of higher density, while the 60S subunit shows a more complex pattern of dense features, comprising a rather globular core, locally extending close to the particle surface.

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